UniProtKB - Q495A1 (TIGIT_HUMAN)
T-cell immunoreceptor with Ig and ITIM domains
TIGIT
Functioni
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
1 PublicationGO - Molecular functioni
- identical protein binding Source: IntAct
- signaling receptor binding Source: MGI
GO - Biological processi
- negative regulation of interleukin-12 production Source: UniProtKB
- negative regulation of T cell activation Source: MGI
- positive regulation of interleukin-10 production Source: UniProtKB
Enzyme and pathway databases
PathwayCommonsi | Q495A1 |
SignaLinki | Q495A1 |
SIGNORi | Q495A1 |
Names & Taxonomyi
Protein namesi | Recommended name: T-cell immunoreceptor with Ig and ITIM domainsAlternative name(s): V-set and immunoglobulin domain-containing protein 9 V-set and transmembrane domain-containing protein 3 |
Gene namesi | Name:TIGIT Synonyms:VSIG9, VSTM3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26838, TIGIT |
MIMi | 612859, gene |
neXtProti | NX_Q495A1 |
VEuPathDBi | HostDB:ENSG00000181847 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cell surface Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 22 – 141 | ExtracellularSequence analysisAdd BLAST | 120 | |
Transmembranei | 142 – 162 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 163 – 244 | CytoplasmicSequence analysisAdd BLAST | 82 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 42 | I → A: Abrogates interaction with PVR, cell clustering and PVR signaling. 1 Publication | 1 | |
Mutagenesisi | 42 | I → D: Abrogates interaction with PVR, cell clustering and PVR signaling. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 201633 |
OpenTargetsi | ENSG00000181847 |
PharmGKBi | PA164726482 |
Miscellaneous databases
Pharosi | Q495A1, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4630878 |
Genetic variation databases
BioMutai | TIGIT |
DMDMi | 121943253 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000299405 | 22 – 244 | T-cell immunoreceptor with Ig and ITIM domainsAdd BLAST | 223 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 32 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 45 ↔ 108 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 101 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | Q495A1 |
MassIVEi | Q495A1 |
PaxDbi | Q495A1 |
PeptideAtlasi | Q495A1 |
PRIDEi | Q495A1 |
ProteomicsDBi | 61948 [Q495A1-1] |
PTM databases
GlyGeni | Q495A1, 2 sites |
iPTMneti | Q495A1 |
PhosphoSitePlusi | Q495A1 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000181847, Expressed in lymph node and 117 other tissues |
ExpressionAtlasi | Q495A1, baseline and differential |
Genevisiblei | Q495A1, HS |
Organism-specific databases
HPAi | ENSG00000181847, Tissue enriched (lymphoid) |
Interactioni
Subunit structurei
Homodimer in cis; binds with high affinity to PVR, forming a heterotetrameric assembly of two TIGIT and two PVR molecules. Binds with lower affinity to NECTIN2 and NECTIN3.
1 PublicationBinary interactionsi
Q495A1
GO - Molecular functioni
- identical protein binding Source: IntAct
- signaling receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 128399, 27 interactors |
IntActi | Q495A1, 7 interactors |
STRINGi | 9606.ENSP00000419085 |
Miscellaneous databases
RNActi | Q495A1, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q495A1 |
SMRi | Q495A1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q495A1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 124 | Ig-like V-typeAdd BLAST | 103 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 32 – 42 | HomodimerizationAdd BLAST | 11 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 229 – 234 | ITIM motif | 6 |
Domaini
Keywords - Domaini
Immunoglobulin domain, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502SQW2, Eukaryota |
GeneTreei | ENSGT00390000012671 |
HOGENOMi | CLU_073090_0_0_1 |
InParanoidi | Q495A1 |
OMAi | EVTQVNW |
OrthoDBi | 1484731at2759 |
PhylomeDBi | Q495A1 |
TreeFami | TF338423 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set IPR042948, TIGIT |
PANTHERi | PTHR47734, PTHR47734, 1 hit |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRWCLLLIWA QGLRQAPLAS GMMTGTIETT GNISAEKGGS IILQCHLSST
60 70 80 90 100
TAQVTQVNWE QQDQLLAICN ADLGWHISPS FKDRVAPGPG LGLTLQSLTV
110 120 130 140 150
NDTGEYFCIY HTYPDGTYTG RIFLEVLESS VAEHGARFQI PLLGAMAATL
160 170 180 190 200
VVICTAVIVV VALTRKKKAL RIHSVEGDLR RKSAGQEEWS PSAPSPPGSC
210 220 230 240
VQAEAAPAGL CGEQRGEDCA ELHDYFNVLS YRSLGNCSFF TETG
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J0B0 | C9J0B0_HUMAN | T-cell immunoreceptor with Ig and I... | TIGIT | 169 | Annotation score: | ||
C9JZW6 | C9JZW6_HUMAN | T-cell immunoreceptor with Ig and I... | TIGIT | 163 | Annotation score: | ||
A0A0C4DGA4 | A0A0C4DGA4_HUMAN | T-cell immunoreceptor with Ig and I... | TIGIT | 311 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 103 | T → A in AAI01289 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 117 | T → A in BAC04973 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_056079 | 33 | I → V. Corresponds to variant dbSNP:rs13098836Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_027634 | 167 – 170 | KKAL → FVCF in isoform 2. 1 Publication | 4 | |
Alternative sequenceiVSP_027635 | 171 – 244 | Missing in isoform 2. 1 PublicationAdd BLAST | 74 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK097192 mRNA Translation: BAC04973.1 AL833175 mRNA Translation: CAI46183.1 Different initiation. BX640915 mRNA Translation: CAE45956.1 BC101288 mRNA Translation: AAI01289.1 BC101289 mRNA Translation: AAI01290.1 BC101290 mRNA Translation: AAI01291.1 BC101291 mRNA Translation: AAI01292.1 |
CCDSi | CCDS2980.1 [Q495A1-1] |
RefSeqi | NP_776160.2, NM_173799.3 [Q495A1-1] |
Genome annotation databases
Ensembli | ENST00000383671.8; ENSP00000373167.3; ENSG00000181847.12 ENST00000486257.5; ENSP00000419085.1; ENSG00000181847.12 |
GeneIDi | 201633 |
KEGGi | hsa:201633 |
MANE-Selecti | ENST00000383671.8; ENSP00000373167.3; NM_173799.4; NP_776160.2 |
UCSCi | uc003ebg.3, human [Q495A1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK097192 mRNA Translation: BAC04973.1 AL833175 mRNA Translation: CAI46183.1 Different initiation. BX640915 mRNA Translation: CAE45956.1 BC101288 mRNA Translation: AAI01289.1 BC101289 mRNA Translation: AAI01290.1 BC101290 mRNA Translation: AAI01291.1 BC101291 mRNA Translation: AAI01292.1 |
CCDSi | CCDS2980.1 [Q495A1-1] |
RefSeqi | NP_776160.2, NM_173799.3 [Q495A1-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3Q0H | X-ray | 1.70 | A/B | 22-137 | [»] | |
3RQ3 | X-ray | 2.70 | A/B | 22-137 | [»] | |
3UCR | X-ray | 2.63 | A/B/C/D | 23-128 | [»] | |
3UDW | X-ray | 2.90 | A/B | 20-128 | [»] | |
5V52 | X-ray | 3.10 | A/B | 22-128 | [»] | |
AlphaFoldDBi | Q495A1 | |||||
SMRi | Q495A1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 128399, 27 interactors |
IntActi | Q495A1, 7 interactors |
STRINGi | 9606.ENSP00000419085 |
Chemistry databases
ChEMBLi | CHEMBL4630878 |
PTM databases
GlyGeni | Q495A1, 2 sites |
iPTMneti | Q495A1 |
PhosphoSitePlusi | Q495A1 |
Genetic variation databases
BioMutai | TIGIT |
DMDMi | 121943253 |
Proteomic databases
jPOSTi | Q495A1 |
MassIVEi | Q495A1 |
PaxDbi | Q495A1 |
PeptideAtlasi | Q495A1 |
PRIDEi | Q495A1 |
ProteomicsDBi | 61948 [Q495A1-1] |
Protocols and materials databases
ABCDi | Q495A1, 190 sequenced antibodies |
Antibodypediai | 53759, 429 antibodies from 28 providers |
DNASUi | 201633 |
Genome annotation databases
Ensembli | ENST00000383671.8; ENSP00000373167.3; ENSG00000181847.12 ENST00000486257.5; ENSP00000419085.1; ENSG00000181847.12 |
GeneIDi | 201633 |
KEGGi | hsa:201633 |
MANE-Selecti | ENST00000383671.8; ENSP00000373167.3; NM_173799.4; NP_776160.2 |
UCSCi | uc003ebg.3, human [Q495A1-1] |
Organism-specific databases
CTDi | 201633 |
DisGeNETi | 201633 |
GeneCardsi | TIGIT |
HGNCi | HGNC:26838, TIGIT |
HPAi | ENSG00000181847, Tissue enriched (lymphoid) |
MIMi | 612859, gene |
neXtProti | NX_Q495A1 |
OpenTargetsi | ENSG00000181847 |
PharmGKBi | PA164726482 |
VEuPathDBi | HostDB:ENSG00000181847 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502SQW2, Eukaryota |
GeneTreei | ENSGT00390000012671 |
HOGENOMi | CLU_073090_0_0_1 |
InParanoidi | Q495A1 |
OMAi | EVTQVNW |
OrthoDBi | 1484731at2759 |
PhylomeDBi | Q495A1 |
TreeFami | TF338423 |
Enzyme and pathway databases
PathwayCommonsi | Q495A1 |
SignaLinki | Q495A1 |
SIGNORi | Q495A1 |
Miscellaneous databases
BioGRID-ORCSi | 201633, 7 hits in 1066 CRISPR screens |
ChiTaRSi | TIGIT, human |
EvolutionaryTracei | Q495A1 |
GenomeRNAii | 201633 |
Pharosi | Q495A1, Tbio |
PROi | PR:Q495A1 |
RNActi | Q495A1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000181847, Expressed in lymph node and 117 other tissues |
ExpressionAtlasi | Q495A1, baseline and differential |
Genevisiblei | Q495A1, HS |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set IPR042948, TIGIT |
PANTHERi | PTHR47734, PTHR47734, 1 hit |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TIGIT_HUMAN | |
Accessioni | Q495A1Primary (citable) accession number: Q495A1 Secondary accession number(s): Q495A3 Q8N877 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | September 13, 2005 | |
Last modified: | May 25, 2022 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references