UniProtKB - Q49575 (A85B_MYCIA)
Diacylglycerol acyltransferase/mycolyltransferase Ag85B
fbpB
Functioni
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).
By similarityCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 166 | NucleophileBy similarity | 1 | |
Binding sitei | 166 | SubstrateBy similarity | 1 | |
Binding sitei | 194 | SubstrateBy similarity | 1 | |
Active sitei | 270 | By similarity | 1 | |
Binding sitei | 279 | SubstrateBy similarity | 1 | |
Active sitei | 302 | By similarity | 1 |
GO - Molecular functioni
- diacylglycerol O-acyltransferase activity Source: UniProtKB-EC
- trehalose O-mycolyltransferase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Acyltransferase, Transferase |
Protein family/group databases
ESTHERi | mycit-a85b, A85-Mycolyl-transferase |
Names & Taxonomyi
Protein namesi | Recommended name: Diacylglycerol acyltransferase/mycolyltransferase Ag85B (EC:2.3.1.122, EC:2.3.1.20)Short name: DGAT Alternative name(s): 30 kDa extracellular protein Acyl-CoA:diacylglycerol acyltransferase Antigen 85 complex B Short name: 85B Short name: Ag85B Extracellular alpha-antigen Fibronectin-binding protein B Short name: Fbps B |
Gene namesi | Name:fbpB Ordered Locus Names:OCU_26350 |
Organismi | Mycobacterium intracellulare (strain ATCC 13950 / DSM 43223 / JCM 6384 / NCTC 13025 / 3600) |
Taxonomic identifieri | 487521 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC) › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 40 | By similarityAdd BLAST | 40 | |
ChainiPRO_0000000218 | 41 – 330 | Diacylglycerol acyltransferase/mycolyltransferase Ag85BAdd BLAST | 290 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 127 ↔ 132 | By similarity |
Keywords - PTMi
Disulfide bondFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 82 – 83 | Substrate bindingBy similarity | 2 | |
Regioni | 98 – 108 | Fibronectin-bindingAdd BLAST | 11 | |
Regioni | 272 – 275 | Substrate bindingBy similarity | 4 | |
Regioni | 302 – 304 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0627, Bacteria |
HOGENOMi | CLU_026624_3_1_11 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase-like |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MTDLSEKVRA WGRRLVVGAA AAATLPGLIG IAGGAATANA FSRPGLPVEY
60 70 80 90 100
LQVPSAGMGR DIKVQFQSGG NGSPAVYLLD GLRAQDDYNG WDINTPAFEW
110 120 130 140 150
YYQSGLSVIM PVGGQSSFYA DWYQPACGKA GCSTYKWETF LTSELPQYLA
160 170 180 190 200
SNKGVKSTGS AAVGISMSGS SAMILAVNHP NQFVYAGSLS ALLDPSQGMG
210 220 230 240 250
PSLIGLAMGD AGGYKADAMW GPSSDPAWQR NDPSLQIPAL VGNNTRLWVY
260 270 280 290 300
CGNGTPSELG GANMPAEFLE NFVRSSNLKF QDAYNAAGGH NAVFNFNANG
310 320 330
THSWEYWGAQ LNAMKPDLQS ALGASSGGGG
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 47 – 48 | PV → EF in BAA03243 (PubMed:8250904).Curated | 2 | |
Sequence conflicti | 138 | E → D in BAA03243 (PubMed:8250904).Curated | 1 | |
Sequence conflicti | 138 | E → D in BAA03981 (PubMed:8250904).Curated | 1 | |
Sequence conflicti | 310 | Q → S in BAA03243 (PubMed:8250904).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D16546 Genomic DNA Translation: BAA03981.1 D14253 Genomic DNA Translation: BAA03243.1 CP003322 Genomic DNA Translation: AFC43854.1 Different initiation. |
PIRi | JN0897 |
RefSeqi | WP_009952996.1, NZ_ABIN01000047.1 |
Genome annotation databases
EnsemblBacteriai | AFC43854; AFC43854; OCU_26350 |
GeneIDi | 66745300 |
KEGGi | mia:OCU_26350 |
PATRICi | fig|487521.10.peg.2648 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D16546 Genomic DNA Translation: BAA03981.1 D14253 Genomic DNA Translation: BAA03243.1 CP003322 Genomic DNA Translation: AFC43854.1 Different initiation. |
PIRi | JN0897 |
RefSeqi | WP_009952996.1, NZ_ABIN01000047.1 |
3D structure databases
SMRi | Q49575 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 487521.OCU_26350 |
Protein family/group databases
ESTHERi | mycit-a85b, A85-Mycolyl-transferase |
Genome annotation databases
EnsemblBacteriai | AFC43854; AFC43854; OCU_26350 |
GeneIDi | 66745300 |
KEGGi | mia:OCU_26350 |
PATRICi | fig|487521.10.peg.2648 |
Phylogenomic databases
eggNOGi | COG0627, Bacteria |
HOGENOMi | CLU_026624_3_1_11 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase-like |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | A85B_MYCIA | |
Accessioni | Q49575Primary (citable) accession number: Q49575 Secondary accession number(s): H8IM05, P94938 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | June 13, 2012 | |
Last modified: | February 23, 2022 | |
This is version 91 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |