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Entry version 111 (11 Dec 2019)
Sequence version 1 (01 Nov 1996)
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Protein

Ethylmalonyl-CoA mutase

Gene

ecm

Organism
Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (Methylobacterium extorquens)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Radical enzyme that catalyzes the transformation of (2R)-ethylmalonyl-CoA to (2S)-methylsuccinyl-CoA (PubMed:25448820). Is involved in the ethylmalonyl-CoA pathway for acetyl-CoA assimilation required for M.extorquens growth on one- and two-carbon compounds such as ethylamine, methanol or ethanol as sole carbon source (PubMed:8868443, PubMed:8704985, PubMed:25448820). This enzyme acts as a regulatory metabolic control point in this pathway, that allows M.extorquens to efficiently restore metabolic balance when challenged with a sudden change in the growth substrate (PubMed:25448820).3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

adenosylcob(III)alamin1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi543Cobalt (cobalamin axial ligand)By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionIsomerase
LigandCobalamin, Cobalt, Metal-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ethylmalonyl-CoA mutase1 Publication (EC:5.4.99.631 Publication)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ecm1 Publication
Synonyms:meaA1 Publication
Ordered Locus Names:MexAM1_META1p0180
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMethylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (Methylobacterium extorquens)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri272630 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylorubrum
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000009081 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Cells lacking this gene lose the ability to grow on C1 (methanol and methylamine) and C2 (ethanol and ethylamine) compounds, and also on beta-hydroxybutyrate, but they grow normally on pyruvate. Growth on C1 and C2 compounds is restored by addition of glyoxylate or glycolate, indicating that these mutants are unable to convert acetyl-CoA into glyoxylate.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001942761 – 688Ethylmalonyl-CoA mutaseAdd BLAST688

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q49115

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Up-regulated during growth on ethylamine.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
272630.MexAM1_META1p0180

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q49115

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini530 – 659B12-bindingPROSITE-ProRule annotationAdd BLAST130

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the methylmalonyl-CoA mutase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105RFZ Bacteria
COG1884 LUCA
COG2185 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000003917

KEGG Orthology (KO)

More...
KOi
K14447

Identification of Orthologs from Complete Genome Data

More...
OMAi
QWSLRMQ

Database of Orthologous Groups

More...
OrthoDBi
154460at2

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006159 Acid_CoA_mut_C
IPR016176 Cbl-dep_enz_cat
IPR006158 Cobalamin-bd
IPR036724 Cobalamin-bd_sf
IPR006099 MeMalonylCoA_mutase_a/b_cat
IPR006098 MMCoA_mutase_a_cat

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02310 B12-binding, 1 hit
PF01642 MM_CoA_mutase, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51703 SSF51703, 1 hit
SSF52242 SSF52242, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00640 acid_CoA_mut_C, 1 hit
TIGR00641 acid_CoA_mut_N, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51332 B12_BINDING, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q49115-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSAQASVAEV KRDKPWIIRT YAGHSTAAES NKLYRGNLAK GQTGLSVAFD
60 70 80 90 100
LPTQTGYDPD HELARGEVGK VGVSIAHLGD MRALFDQIPL AQMNTSMTIN
110 120 130 140 150
ATAPWLLSLY LAVAEEQGAP LAALQGTTQN DIIKEYLSRG TYVFPPAPSL
160 170 180 190 200
RLTKDVILFT TKNVPKWNPM NVCSYHLQEA GATPVQELSY ALAIAIAVLD
210 220 230 240 250
TVRDDPDFDE ASFSDVFSRI SFFVNAGMRF VTEICKMRAF AELWDEIAQE
260 270 280 290 300
RYGITDAKKR IFRYGVQVNS LGLTEQQPEN NVHRILIEML AVTLSKRARA
310 320 330 340 350
RAVQLPAWNE ALGLPRPWDQ QWSMRMQQIL AFETDLLEYD DIFDGSTVIE
360 370 380 390 400
ARVEALKEQT RAELTRIAEI GGAVTAVEAG ELKRALVESN ARRISAIEKG
410 420 430 440 450
EQIVVGVNKW QQGEPSPLTA GDGAIFTVSE TVEMEAETRI REWRSKRDER
460 470 480 490 500
AVGQALADLE QAARSGANIM PPSIAAAKAG VTTGEWGQRL REVFGEYRAP
510 520 530 540 550
TGVTLQTVTS GAAEDARLLI ADLGERLGET PRLVVGKPGL DGHSNGAEQI
560 570 580 590 600
ALRARDVGFD VTYDGIRQTP TEIVAKAKER GAHVIGLSVL SGSHVPLVRE
610 620 630 640 650
VKAKLREAGL DHVPVVVGGI ISTEDELVLK NMGVTAVYTP KDYELDKIMV
660 670 680
GLAKVVERAL DKRAADRADT EAGVPGAPKR NESGAQVF
Length:688
Mass (Da):75,066
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD84BD366933C0403
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U28335 Genomic DNA Translation: AAC44087.1
CP001510 Genomic DNA Translation: ACS38142.1

NCBI Reference Sequences

More...
RefSeqi
WP_003597279.1, NC_012808.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
ACS38142; ACS38142; MexAM1_META1p0180

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mea:Mex_1p0180

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28335 Genomic DNA Translation: AAC44087.1
CP001510 Genomic DNA Translation: ACS38142.1
RefSeqiWP_003597279.1, NC_012808.1

3D structure databases

SMRiQ49115
ModBaseiSearch...

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p0180

Proteomic databases

PRIDEiQ49115

Genome annotation databases

EnsemblBacteriaiACS38142; ACS38142; MexAM1_META1p0180
KEGGimea:Mex_1p0180

Phylogenomic databases

eggNOGiENOG4105RFZ Bacteria
COG1884 LUCA
COG2185 LUCA
HOGENOMiHOG000003917
KOiK14447
OMAiQWSLRMQ
OrthoDBi154460at2

Family and domain databases

InterProiView protein in InterPro
IPR006159 Acid_CoA_mut_C
IPR016176 Cbl-dep_enz_cat
IPR006158 Cobalamin-bd
IPR036724 Cobalamin-bd_sf
IPR006099 MeMalonylCoA_mutase_a/b_cat
IPR006098 MMCoA_mutase_a_cat
PfamiView protein in Pfam
PF02310 B12-binding, 1 hit
PF01642 MM_CoA_mutase, 1 hit
SUPFAMiSSF51703 SSF51703, 1 hit
SSF52242 SSF52242, 1 hit
TIGRFAMsiTIGR00640 acid_CoA_mut_C, 1 hit
TIGR00641 acid_CoA_mut_N, 1 hit
PROSITEiView protein in PROSITE
PS51332 B12_BINDING, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiECM_METEA
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q49115
Secondary accession number(s): C5AP83
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: December 11, 2019
This is version 111 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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