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UniProtKB - Q48436 (BUDC_KLEPN)
Protein
Diacetyl reductase [(S)-acetoin forming]
Gene
budC
Organism
Klebsiella pneumoniae
Status
Functioni
Catalyzes the reversible reduction of (S)-acetoin to 2,3-butanediol in the presence of NADH.
Catalytic activityi
- EC:1.1.1.304
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 59 | NAD1 Publication | 1 | |
Binding sitei | 139 | Substrate | 1 | |
Active sitei | 152 | Proton acceptor | 1 | |
Binding sitei | 156 | NAD1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 6 – 33 | NAD1 PublicationAdd BLAST | 28 |
GO - Molecular functioni
- diacetyl reductase ((S)-acetoin forming) activity Source: UniProtKB-EC
GO - Biological processi
- acetoin catabolic process Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Enzyme and pathway databases
BRENDAi | 1.1.1.304, 2814 |
Names & Taxonomyi
Protein namesi | Recommended name: Diacetyl reductase [(S)-acetoin forming] (EC:1.1.1.304)Alternative name(s): Acetoin(diacetyl) reductase Short name: AR Meso-2,3-butanediol dehydrogenase |
Gene namesi | Name:budC |
Organismi | Klebsiella pneumoniae |
Taxonomic identifieri | 573 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Klebsiella |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054537 | 1 – 256 | Diacetyl reductase [(S)-acetoin forming]Add BLAST | 256 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q48436 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q48436 |
Family & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Phylogenomic databases
OrthoDBi | 1601931at2 |
Family and domain databases
InterProi | View protein in InterPro IPR014007, 23BDH IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02415, 23BDH, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q48436-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKVALVTGA GQGIGKAIAL RLVKDGFAVA IADYNDATAK AVASEINQAG
60 70 80 90 100
GRAMAVKVDV SDRDQVFAAV EQARKTLGGF DVIVNNAGVA PSTPIESITP
110 120 130 140 150
EIVDKVYNIN VKGVIWGIQA AVEAFKKEGH GGKIINACSQ AGHVGNPELA
160 170 180 190 200
VYSSSKFAVR GLTQTAARDL APLGITVNGY CPGIVKTPMW AEIDRQVSEA
210 220 230 240 250
AGKPLGYGTA EFAKRITLGR LSEPEDVAAC VSYLASPDSD YMTGQSLLID
GGMVFN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 52 – 54 | RAM → HAV in BAA13085 (Ref. 1) Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86412 Genomic DNA Translation: BAA13085.1 AF098800 Genomic DNA Translation: AAC78679.1 |
RefSeqi | WP_004151179.1, NZ_WYAM01000009.1 |
Genome annotation databases
GeneIDi | 64294969 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86412 Genomic DNA Translation: BAA13085.1 AF098800 Genomic DNA Translation: AAC78679.1 |
RefSeqi | WP_004151179.1, NZ_WYAM01000009.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GEG | X-ray | 1.70 | A/B/C/D/E/F/G/H | 1-256 | [»] | |
3WYE | X-ray | 1.58 | A/B | 1-83 | [»] | |
A/B | 119-134 | [»] | ||||
A/B | 162-181 | [»] | ||||
A/B | 237-256 | [»] | ||||
SMRi | Q48436 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
DrugBanki | DB02379, Beta-D-Glucose |
Genome annotation databases
GeneIDi | 64294969 |
Phylogenomic databases
OrthoDBi | 1601931at2 |
Enzyme and pathway databases
BRENDAi | 1.1.1.304, 2814 |
Miscellaneous databases
EvolutionaryTracei | Q48436 |
Family and domain databases
InterProi | View protein in InterPro IPR014007, 23BDH IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02415, 23BDH, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BUDC_KLEPN | |
Accessioni | Q48436Primary (citable) accession number: Q48436 Secondary accession number(s): Q9R878 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | April 11, 2003 | |
Last modified: | February 23, 2022 | |
This is version 124 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families