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Entry version 122 (10 Feb 2021)
Sequence version 1 (01 Nov 1996)
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Protein

Temperature-sensitive hemagglutinin tsh autotransporter

Gene

tsh

Organism
Escherichia coli
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Contributes to the development of lesions and deposition of fibrin in the avian air sacs. It can act both as an adhesin and as a serine protease. Agglutinates erythrocytes while in contact with the extracellular surface of the bacterial cells. Can adhere to purified hemoglobin and bind with great efficiency to extracellular matrix proteins. Cleaves casein and exhibits mucinolytic activity.4 Publications

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Temperature dependencei

Optimum temperature is 26 degrees Celsius for hemagglutination activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei125Charge relay systemPROSITE-ProRule annotation1
Active sitei153Charge relay systemPROSITE-ProRule annotation1
Active sitei259Charge relay system1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processVirulence

Protein family/group databases

MEROPS protease database

More...
MEROPSi
S06.003

Transport Classification Database

More...
TCDBi
1.B.12.4.2, the autotransporter-1 (at-1) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Temperature-sensitive hemagglutinin tsh autotransporter (EC:3.4.21.-)
Alternative name(s):
Autotransporter tsh
Cleaved into the following 2 chains:
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:tsh
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates if the gene coding for the protein originates from the hydrogenosome, the mitochondrion, the nucleomorph, different plastids or a plasmid. The absence of this section means that the gene is located in one of the main chromosomal element(s).<p><a href='/help/encoded_on' target='_top'>More...</a></p>Encoded oniPlasmid pAPEC-13 Publications
Plasmid pAPEC-O2-ColV1 Publication
Plasmid pYA34321 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri562 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi259S → A: Loss of proteolytic activity. No effect on hemagglutination activity. 2 Publications1
Mutagenesisi259S → T: No effect either on extracellular secretion or on hemagglutination activity. 2 Publications1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 521 PublicationAdd BLAST52
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000038761053 – 1377Temperature-sensitive hemagglutinin tsh autotransporterAdd BLAST1325
ChainiPRO_000002698253 – 1100Temperature-sensitive hemagglutinin tshAdd BLAST1048
ChainiPRO_00000269831101 – 1377Temperature-sensitive hemagglutinin tsh translocatorAdd BLAST277

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

The C-terminus is blocked.Curated
Cleaved to release the mature protein from the outer membrane.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei1100 – 1101Cleavage2

Keywords - PTMi

Zymogen

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q47692

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q47692

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini53 – 302Peptidase S6PROSITE-ProRule annotationAdd BLAST250
Domaini1111 – 1377AutotransporterPROSITE-ProRule annotationAdd BLAST267

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Adhesive and proteolytic functions of tsh lie in different domains of the protein.
The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.160.20.20, 1 hit
2.40.128.130, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR005546, Autotransporte_beta
IPR036709, Autotransporte_beta_dom_sf
IPR006315, OM_autotransptr_brl
IPR012332, P22_tailspike-like_C_sf
IPR011050, Pectin_lyase_fold/virulence
IPR009003, Peptidase_S1_PA
IPR000710, Peptidase_S6
IPR030396, Peptidase_S6_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03797, Autotransporter, 1 hit
PF02395, Peptidase_S6, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00921, IGASERPTASE

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00869, Autotransporter, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF103515, SSF103515, 1 hit
SSF50494, SSF50494, 1 hit
SSF51126, SSF51126, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01414, autotrans_barl, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51208, AUTOTRANSPORTER, 1 hit
PS51691, PEPTIDASE_S6, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

Q47692-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNRIYSLRYS AVARGFIAVS EFARKCVHKS VRRLCFPVLL LIPVLFSAGS
60 70 80 90 100
LAGTVNNELG YQLFRDFAEN KGMFRPGATN IAIYNKQGEF VGTLDKAAMP
110 120 130 140 150
DFSAVDSEIG VATLINPQYI ASVKHNGGYT NVSFGDGENR YNIVDRNNAP
160 170 180 190 200
SLDFHAPRLD KLVTEVAPTA VTAQGAVAGA YLDKERYPVF YRLGSGTQYI
210 220 230 240 250
KDSNGQLTQM GGAYSWLTGG TVGSLSSYQN GEMISTSSGL VFDYKLNGAM
260 270 280 290 300
PIYGEAGDSG SPLFAFDTVQ NKWVLVGVLT AGNGAGGRGN NWAVIPLDFI
310 320 330 340 350
GQKFNEDNDA PVTFRTSEGG ALEWSFNSST GAGALTQGTT TYAMHGQQGN
360 370 380 390 400
DLNAGKNLIF QGQNGQINLK DSVSQGAGSL TFRDNYTVTT SNGSTWTGAG
410 420 430 440 450
IVVDNGVSVN WQVNGVKGDN LHKIGEGTLT VQGTGINEGG LKVGDGKVVL
460 470 480 490 500
NQQADNKGQV QAFSSVNIAS GRPTVVLTDE RQVNPDTVSW GYRGGTLDVN
510 520 530 540 550
GNSLTFHQLK AADYGAVLAN NVDKRATITL DYALRADKVA LNGWSESGKG
560 570 580 590 600
TAGNLYKYNN PYTNTTDYFI LKQSTYGYFP TDQSSNATWE FVGHSQGDAQ
610 620 630 640 650
KLVADRFNTA GYLFHGQLKG NLNVDNRLPE GVTGALVMDG AADISGTFTQ
660 670 680 690 700
ENGRLTLQGH PVIHAYNTQS VADKLAASGD HSVLTQPTSF SQEDWENRSF
710 720 730 740 750
TFDRLSLKNT DFGLGRNATL NTTIQADNSS VTLGDSRVFI DKNDGQGTAF
760 770 780 790 800
TLEEGTSVAT KDADKSVFNG TVNLDNQSVL NINDIFNGGI QANNSTVNIS
810 820 830 840 850
SDSAVLGNST LTSTALNLNK GANALASQSF VSDGPVNISD AALSLNSRPD
860 870 880 890 900
EVSHTLLPVY DYAGSWNLKG DDARLNVGPY SMLSGNINVQ DKGTVTLGGE
910 920 930 940 950
GELSPDLTLQ NQMLYSLFNG YRNIWSGSLN APDATVSMTD TQWSMNGNST
960 970 980 990 1000
AGNMKLNRTI VGFNGGTSPF TTLTTDNLDA VQSAFVMRTD LNKADKLVIN
1010 1020 1030 1040 1050
KSATGHDNSI WVNFLKKPSN KDTLDIPLVS APEATADNLF RASTRVVGFS
1060 1070 1080 1090 1100
DVTPILSVRK EDGKKEWVLD GYQVARNDGQ GKAAATFMHI SYNNFITEVN
1110 1120 1130 1140 1150
NLNKRMGDLR DINGEAGTWV RLLNGSGSAD GGFTDHYTLL QMGADRKHEL
1160 1170 1180 1190 1200
GSMDLFTGVM ATYTDTDASA DLYSGKTKSW GGGFYASGLF RSGAYFDVIA
1210 1220 1230 1240 1250
KYIHNENKYD LNFAGAGKQN FRSHSLYAGA EVGYRYHLTD TTFVEPQAEL
1260 1270 1280 1290 1300
VWGRLQGQTF NWNDSGMDVS MRRNSVNPLV GRTGVVSGKT FSGKDWSLTA
1310 1320 1330 1340 1350
RAGLHYEFDL TDSADVHLKD AAGEHQINGR KDSRMLYGVG LNARFGDNTR
1360 1370
LGLEVERSAF GKYNTDDAIN ANIRYSF
Length:1,377
Mass (Da):148,227
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i237423644D9AE012
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural varianti175G → W in strain: APEC13. 1
Natural varianti183D → G in strain: APEC13. 1
Natural varianti209Q → K in strain: A2363 and APEC13. 1
Natural varianti326F → S in strain: A2363. 1
Natural varianti422H → Y in strain: A2363. 1
Natural varianti634G → S in strain: A2363. 1
Natural varianti842A → T in strain: A2363. 1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF218073 Genomic DNA Translation: AAA24698.1
AY545598 Genomic DNA Translation: AAT35241.2
AY280856 Genomic DNA Translation: AAP33781.1

Protein sequence database of the Protein Information Resource

More...
PIRi
I54632

NCBI Reference Sequences

More...
RefSeqi
WP_001080137.1, NZ_WVUZ01000043.1
WP_011402691.1, NC_007675.1
YP_444132.1, NC_007675.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218073 Genomic DNA Translation: AAA24698.1
AY545598 Genomic DNA Translation: AAT35241.2
AY280856 Genomic DNA Translation: AAP33781.1
PIRiI54632
RefSeqiWP_001080137.1, NZ_WVUZ01000043.1
WP_011402691.1, NC_007675.1
YP_444132.1, NC_007675.1

3D structure databases

SMRiQ47692
ModBaseiSearch...

Protein family/group databases

MEROPSiS06.003
TCDBi1.B.12.4.2, the autotransporter-1 (at-1) family

Proteomic databases

PRIDEiQ47692

Family and domain databases

Gene3Di2.160.20.20, 1 hit
2.40.128.130, 1 hit
InterProiView protein in InterPro
IPR005546, Autotransporte_beta
IPR036709, Autotransporte_beta_dom_sf
IPR006315, OM_autotransptr_brl
IPR012332, P22_tailspike-like_C_sf
IPR011050, Pectin_lyase_fold/virulence
IPR009003, Peptidase_S1_PA
IPR000710, Peptidase_S6
IPR030396, Peptidase_S6_dom
PfamiView protein in Pfam
PF03797, Autotransporter, 1 hit
PF02395, Peptidase_S6, 1 hit
PRINTSiPR00921, IGASERPTASE
SMARTiView protein in SMART
SM00869, Autotransporter, 1 hit
SUPFAMiSSF103515, SSF103515, 1 hit
SSF50494, SSF50494, 1 hit
SSF51126, SSF51126, 1 hit
TIGRFAMsiTIGR01414, autotrans_barl, 1 hit
PROSITEiView protein in PROSITE
PS51208, AUTOTRANSPORTER, 1 hit
PS51691, PEPTIDASE_S6, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTSH_ECOLX
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q47692
Secondary accession number(s): Q6QE02, Q83WR9
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: February 10, 2021
This is version 122 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid
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