UniProtKB - Q47679 (YAFV_ECOLI)
Protein
Omega-amidase YafV
Gene
yafV
Organism
Escherichia coli (strain K12)
Status
Functioni
Hydrolyzes alpha-ketoglutaramate (a-KGM) to alpha-ketoglutarate (alpha-KG) and ammonia, has weak activity on L-glutamine, almost no activity on deaminated glutathione (dGSH) and none on glutathione (By similarity). May function as a metabolite repair enzyme (By similarity).By similarity
Catalytic activityi
- EC:3.5.1.3By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 42 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Active sitei | 107 | Proton donorPROSITE-ProRule annotation | 1 | |
Active sitei | 141 | NucleophilePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 2-oxoglutaramate amidase activity Source: EcoCyc
- omega-amidase activity Source: EcoCyc
GO - Biological processi
- nitrogen compound metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
BioCyci | EcoCyc:G6103-MONOMER MetaCyc:G6103-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:yafV Ordered Locus Names:b0219, JW5019 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213257 | 1 – 256 | Omega-amidase YafVAdd BLAST | 256 |
Proteomic databases
jPOSTi | Q47679 |
PaxDbi | Q47679 |
PRIDEi | Q47679 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261824, 18 interactors |
DIPi | DIP-11225N |
STRINGi | 511145.b0219 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 234 | CN hydrolasePROSITE-ProRule annotationAdd BLAST | 231 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0388, Bacteria |
HOGENOMi | CLU_030130_3_7_6 |
InParanoidi | Q47679 |
PhylomeDBi | Q47679 |
Family and domain databases
Gene3Di | 3.60.110.10, 1 hit |
InterProi | View protein in InterPro IPR003010, C-N_Hydrolase IPR036526, C-N_Hydrolase_sf IPR001110, UPF0012_CS |
Pfami | View protein in Pfam PF00795, CN_hydrolase, 1 hit |
SUPFAMi | SSF56317, SSF56317, 1 hit |
PROSITEi | View protein in PROSITE PS50263, CN_HYDROLASE, 1 hit PS01227, UPF0012, 1 hit |
i Sequence
Sequence statusi: Complete.
Q47679-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPGLKITLLQ QPLVWMDGPA NLRHFDRQLE GITGRDVIVL PEMFTSGFAM
60 70 80 90 100
EAAASSLAQD DVVNWMTAKA QQCNALIAGS VALQTESGSV NRFLLVEPGG
110 120 130 140 150
TVHFYDKRHL FRMADEHLHY KAGNARVIVE WRGWRILPLV CYDLRFPVWS
160 170 180 190 200
RNLNDYDLAL YVANWPAPRS LHWQALLTAR AIENQAYVAG CNRVGSDGNG
210 220 230 240 250
CHYRGDSRVI NPQGEIIATA DAHQATRIDA ELSMAALREY REKFPAWQDA
DEFRLW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U70214 Genomic DNA Translation: AAB08641.1 U00096 Genomic DNA Translation: AAC73323.1 AP009048 Genomic DNA Translation: BAA77889.2 |
PIRi | D64746 |
RefSeqi | NP_414754.1, NC_000913.3 WP_001118036.1, NZ_SSZK01000029.1 |
Genome annotation databases
EnsemblBacteriai | AAC73323; AAC73323; b0219 BAA77889; BAA77889; BAA77889 |
GeneIDi | 57728908 946585 |
KEGGi | ecj:JW5019 eco:b0219 |
PATRICi | fig|1411691.4.peg.2064 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U70214 Genomic DNA Translation: AAB08641.1 U00096 Genomic DNA Translation: AAC73323.1 AP009048 Genomic DNA Translation: BAA77889.2 |
PIRi | D64746 |
RefSeqi | NP_414754.1, NC_000913.3 WP_001118036.1, NZ_SSZK01000029.1 |
3D structure databases
SMRi | Q47679 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261824, 18 interactors |
DIPi | DIP-11225N |
STRINGi | 511145.b0219 |
Proteomic databases
jPOSTi | Q47679 |
PaxDbi | Q47679 |
PRIDEi | Q47679 |
Genome annotation databases
EnsemblBacteriai | AAC73323; AAC73323; b0219 BAA77889; BAA77889; BAA77889 |
GeneIDi | 57728908 946585 |
KEGGi | ecj:JW5019 eco:b0219 |
PATRICi | fig|1411691.4.peg.2064 |
Organism-specific databases
EchoBASEi | EB3118 |
Phylogenomic databases
eggNOGi | COG0388, Bacteria |
HOGENOMi | CLU_030130_3_7_6 |
InParanoidi | Q47679 |
PhylomeDBi | Q47679 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6103-MONOMER MetaCyc:G6103-MONOMER |
Miscellaneous databases
PROi | PR:Q47679 |
Family and domain databases
Gene3Di | 3.60.110.10, 1 hit |
InterProi | View protein in InterPro IPR003010, C-N_Hydrolase IPR036526, C-N_Hydrolase_sf IPR001110, UPF0012_CS |
Pfami | View protein in Pfam PF00795, CN_hydrolase, 1 hit |
SUPFAMi | SSF56317, SSF56317, 1 hit |
PROSITEi | View protein in PROSITE PS50263, CN_HYDROLASE, 1 hit PS01227, UPF0012, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YAFV_ECOLI | |
Accessioni | Q47679Primary (citable) accession number: Q47679 Secondary accession number(s): Q9R2E1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families