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Entry version 107 (13 Nov 2019)
Sequence version 2 (30 Nov 2010)
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Protein

Glycine betaine/proline/ectoine/pipecolic acid transporter OusA

Gene

ousA

Organism
Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937))
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in uptake and accumulation of various osmoprotectants. Allows the uptake of glycine betaine, proline, ectoine, and pipecolic acid (PubMed:8550465, PubMed:16000740). May be a contributory factor in the infection progression within the host (PubMed:15720084).3 Publications

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=50 µM for glycine betaine1 Publication
  1. Vmax=270 nmol/min/mg enzyme1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processAmino-acid transport, Stress response, Symport, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
DDAD198628:DDA3937_RS07140-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Glycine betaine/proline/ectoine/pipecolic acid transporter OusACurated
Alternative name(s):
Osmoprotectant uptake system A1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ousA1 Publication
Ordered Locus Names:Dda3937_00791
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937))
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri198628 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaeDickeya
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006859 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 38Cytoplasmic1 PublicationAdd BLAST38
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei39 – 59HelicalSequence analysisAdd BLAST21
Topological domaini60 – 66PeriplasmicCurated7
Transmembranei67 – 87HelicalSequence analysisAdd BLAST21
Topological domaini88 – 98CytoplasmicCuratedAdd BLAST11
Transmembranei99 – 119HelicalSequence analysisAdd BLAST21
Topological domaini120 – 122PeriplasmicCurated3
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Topological domaini144 – 170CytoplasmicCuratedAdd BLAST27
Transmembranei171 – 191HelicalSequence analysisAdd BLAST21
Topological domaini192 – 195PeriplasmicCurated4
Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
Topological domaini217 – 261CytoplasmicCuratedAdd BLAST45
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Topological domaini283 – 298PeriplasmicCuratedAdd BLAST16
Transmembranei299 – 319HelicalSequence analysisAdd BLAST21
Topological domaini320 – 326CytoplasmicCurated7
Transmembranei327 – 347HelicalSequence analysisAdd BLAST21
Topological domaini348 – 350PeriplasmicCurated3
Transmembranei351 – 371HelicalSequence analysisAdd BLAST21
Topological domaini372 – 391CytoplasmicCuratedAdd BLAST20
Transmembranei392 – 412HelicalSequence analysisAdd BLAST21
Topological domaini413 – 417PeriplasmicCurated5
Transmembranei418 – 438HelicalSequence analysisAdd BLAST21
Topological domaini439 – 501CytoplasmicCuratedAdd BLAST63

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Disruption of the gene enhances aggressiveness of the bacterium. Under anaerobic conditions, disruption increases pectate lyase (Pel) production and increases the maceration efficiency on potato tubers (PubMed:15720084). Uptake of glycine betaine and choline is completely abolished in the ousA-ousB double mutant (PubMed:16000740).2 Publications

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000503231 – 501Glycine betaine/proline/ectoine/pipecolic acid transporter OusAAdd BLAST501

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Induced by osmotic strength and repressed by osmoprotectants. Expression is not affected by the growth phase.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
198628.Dda3937_00791

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili461 – 495Sequence analysisAdd BLAST35

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105CSH Bacteria
ENOG410XP7I LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000141611

KEGG Orthology (KO)

More...
KOi
K03762

Identification of Orthologs from Complete Genome Data

More...
OMAi
DKFGRQK

Database of Orthologous Groups

More...
OrthoDBi
961781at2

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR020846 MFS_dom
IPR005828 MFS_sugar_transport-like
IPR036259 MFS_trans_sf
IPR004736 MHS_symport
IPR015041 Osmo_CC
IPR036292 ProP_C
IPR005829 Sugar_transporter_CS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF08946 Osmo_CC, 1 hit
PF00083 Sugar_tr, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF103473 SSF103473, 1 hit
SSF103661 SSF103661, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00883 2A0106, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50850 MFS, 1 hit
PS00216 SUGAR_TRANSPORT_1, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q47421-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLKRKRVKP IALDDVTIID DGRLRKAITA AALGNAMEWF DFGVYGFVAY
60 70 80 90 100
ALGQVFFPGA DPGVQMIAAL ATFSVPFLIR PLGGVFFGAL GDKYGRQKIL
110 120 130 140 150
AITIIIMSIS TFCIGLIPSY ERIGIWAPIL LLLAKMAQGF SVGGEYTGAS
160 170 180 190 200
IFVAEYSPDR KRGFMGSWLD FGSIAGFVLG AGVVVLISTL IGEQAFLAWG
210 220 230 240 250
WRLPFFLALP LGLIGLYLRH ALEETPAFRQ HVEKLEQNDR DGLKAGPGVS
260 270 280 290 300
FREIATHHWK SLLVCIGLVI ATNVTYYMLL TYMPSYLSHS LHYSENHGVL
310 320 330 340 350
IIIAIMIGML FVQPVMGLLS DRFGRKPFVV IGSVAMFFLA VPSFMLINSD
360 370 380 390 400
IIGLIFLGLL MLAVILNAFT GVMASTLPAL FPTHIRYSAL ASAFNISVLI
410 420 430 440 450
AGLTPTVAAW LVESSQNLYM PAYYLMVIAV IGLLTGLFMK ETANKPLKGA
460 470 480 490 500
TPAASDLSEA KEILQEHHDN IEHKIEDITQ QIAELEAKRQ LLVAQHPRIN

D
Length:501
Mass (Da):54,858
Last modified:November 30, 2010 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7493159C53291F66
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti60Missing in CAA57718 (PubMed:8550465).Curated1
Sequence conflicti66M → S in CAA57718 (PubMed:8550465).Curated1
Sequence conflicti79 – 80IR → M in CAA57718 (PubMed:8550465).Curated2
Sequence conflicti219 – 221RHA → AT in CAA57718 (PubMed:8550465).Curated3

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X82267 Genomic DNA Translation: CAA57718.1
CP002038 Genomic DNA Translation: ADM97704.1

NCBI Reference Sequences

More...
RefSeqi
WP_013317165.1, NC_014500.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
ADM97704; ADM97704; Dda3937_00791

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ddd:Dda3937_00791

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|198628.6.peg.1498

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82267 Genomic DNA Translation: CAA57718.1
CP002038 Genomic DNA Translation: ADM97704.1
RefSeqiWP_013317165.1, NC_014500.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi198628.Dda3937_00791

Genome annotation databases

EnsemblBacteriaiADM97704; ADM97704; Dda3937_00791
KEGGiddd:Dda3937_00791
PATRICifig|198628.6.peg.1498

Phylogenomic databases

eggNOGiENOG4105CSH Bacteria
ENOG410XP7I LUCA
HOGENOMiHOG000141611
KOiK03762
OMAiDKFGRQK
OrthoDBi961781at2

Enzyme and pathway databases

BioCyciDDAD198628:DDA3937_RS07140-MONOMER

Family and domain databases

InterProiView protein in InterPro
IPR020846 MFS_dom
IPR005828 MFS_sugar_transport-like
IPR036259 MFS_trans_sf
IPR004736 MHS_symport
IPR015041 Osmo_CC
IPR036292 ProP_C
IPR005829 Sugar_transporter_CS
PfamiView protein in Pfam
PF08946 Osmo_CC, 1 hit
PF00083 Sugar_tr, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
SSF103661 SSF103661, 1 hit
TIGRFAMsiTIGR00883 2A0106, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit
PS00216 SUGAR_TRANSPORT_1, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiOUSA_DICD3
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q47421
Secondary accession number(s): E0SGQ5
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 30, 2010
Last modified: November 13, 2019
This is version 107 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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