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UniProtKB - Q47319 (TAPT_ECOLI)
Protein
tRNA-uridine aminocarboxypropyltransferase
Gene
tapT
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine (acp3U) at position 47 of tRNAs (PubMed:31804502, PubMed:31863583, PubMed:4597321).
Acp3U47 confers thermal stability on tRNA (PubMed:31804502).
3 PublicationsCatalytic activityi
- a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-amino-3-carboxypropyl]uridine in tRNA + H+ + S-methyl-5'-thioadenosine3 PublicationsEC:2.5.1.253 PublicationsThis reaction proceeds in the forward3 Publications direction.
- S-adenosyl-L-methionine + uridine47 in tRNAPhe = 3-[(3S)-3-amino-3-carboxypropyl]uridine47 in tRNAPhe + H+ + S-methyl-5'-thioadenosine1 PublicationEC:2.5.1.251 PublicationThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
The degree of the acp3U modification at U47 is dependent on the presence of the m7G modification at the preceding nucleotide G46. It also depends on medium conditions.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 31 | Zinc1 Publication | 1 | |
Metal bindingi | 34 | Zinc1 Publication | 1 | |
Metal bindingi | 41 | Zinc1 Publication | 1 | |
Metal bindingi | 43 | Zinc1 Publication | 1 |
GO - Molecular functioni
- tRNA-uridine aminocarboxypropyltransferase activity Source: UniProtKB
- zinc ion binding Source: EcoCyc
GO - Biological processi
- tRNA modification Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | tRNA processing |
Ligand | Metal-binding, S-adenosyl-L-methionine, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G7349-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA-uridine aminocarboxypropyltransferaseCurated (EC:2.5.1.253 Publications)Alternative name(s): SAM-dependent 3-amino-3-carboxypropyl transferase1 Publication tRNA U47 acp transferase A1 Publication tRNA aminocarboxypropyltransferase1 Publication |
Gene namesi | Name:tapT1 Publication Synonyms:tuaA1 Publication, yfiP Ordered Locus Names:b2583, JW5409 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Deletion of the gene leads to the loss of the acp3U modification (PubMed:31804502, PubMed:31863583). Mutant has no obvious defect under normal growth conditions (PubMed:31863583). It shows motility defect and genome instability under continuous heat stress (PubMed:31804502).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 – 20 | Missing : Complete loss of activity. 1 PublicationAdd BLAST | 20 | |
Mutagenesisi | 31 | C → S: Complete loss of activity; when associated with S-34. 1 Publication | 1 | |
Mutagenesisi | 34 | C → S: Complete loss of activity; when associated with S-31. 1 Publication | 1 | |
Mutagenesisi | 41 | C → S: Shows weak residual activity; when associated with S-43. 1 Publication | 1 | |
Mutagenesisi | 43 | C → S: Shows weak residual activity; when associated with S-41. 1 Publication | 1 | |
Mutagenesisi | 137 | D → A: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 140 | W → A: Strong decrease in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169271 | 1 – 232 | tRNA-uridine aminocarboxypropyltransferaseAdd BLAST | 232 |
Proteomic databases
PaxDbi | Q47319 |
PRIDEi | Q47319 |
Interactioni
Subunit structurei
Monomer in solution.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4261901, 5 interactors 851395, 1 interactor |
DIPi | DIP-12067N |
IntActi | Q47319, 2 interactors |
STRINGi | 511145.b2583 |
Structurei
3D structure databases
AlphaFoldDBi | Q47319 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 137 – 140 | DXTWCurated | 4 |
Domaini
Contains 1 DXTW motif (PubMed:31863583). The N-terminal domain binds zinc and is important for activity (PubMed:31863583).1 Publication1 Publication
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG3148, Bacteria |
HOGENOMi | CLU_066458_1_0_6 |
InParanoidi | Q47319 |
OMAi | WQPYVVF |
PhylomeDBi | Q47319 |
Family and domain databases
InterProi | View protein in InterPro IPR005636, DTW IPR039262, DTWD2/YfiP |
PANTHERi | PTHR21392, PTHR21392, 1 hit |
Pfami | View protein in Pfam PF03942, DTW, 1 hit |
SMARTi | View protein in SMART SM01144, DTW, 1 hit |
i Sequence
Sequence statusi: Complete.
Q47319-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTENAVLQLR AERIARATRP FLARGNRVRR CQRCLLPEKL CLCSTITPAQ
60 70 80 90 100
AKSRFCLLMF DTEPMKPSNT GRLIADILPD TVAFQWSRTE PSQDLLELVQ
110 120 130 140 150
NPDYQPMVVF PASYADEQRE VIFTPPAGKP PLFIMLDGTW PEARKMFRKS
160 170 180 190 200
PYLDNLPVIS VDLSRLSAYR LREAQAEGQY CTAEVAIALL DMAGDTGAAA
210 220 230
GLGEHFTRFK TRYLAGKTQH LGSITAEQLE SV
Sequence cautioni
The sequence BAA10924 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D64044 Genomic DNA Translation: BAA10924.1 Different initiation. U00096 Genomic DNA Translation: AAC75636.2 AP009048 Genomic DNA Translation: BAE76753.1 |
PIRi | F65036 |
RefSeqi | NP_417078.4, NC_000913.3 WP_001300438.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75636; AAC75636; b2583 BAE76753; BAE76753; BAE76753 |
GeneIDi | 947057 |
KEGGi | ecj:JW5409 eco:b2583 |
PATRICi | fig|511145.12.peg.2686 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D64044 Genomic DNA Translation: BAA10924.1 Different initiation. U00096 Genomic DNA Translation: AAC75636.2 AP009048 Genomic DNA Translation: BAE76753.1 |
PIRi | F65036 |
RefSeqi | NP_417078.4, NC_000913.3 WP_001300438.1, NZ_LN832404.1 |
3D structure databases
AlphaFoldDBi | Q47319 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4261901, 5 interactors 851395, 1 interactor |
DIPi | DIP-12067N |
IntActi | Q47319, 2 interactors |
STRINGi | 511145.b2583 |
Proteomic databases
PaxDbi | Q47319 |
PRIDEi | Q47319 |
Genome annotation databases
EnsemblBacteriai | AAC75636; AAC75636; b2583 BAE76753; BAE76753; BAE76753 |
GeneIDi | 947057 |
KEGGi | ecj:JW5409 eco:b2583 |
PATRICi | fig|511145.12.peg.2686 |
Organism-specific databases
EchoBASEi | EB3975 |
Phylogenomic databases
eggNOGi | COG3148, Bacteria |
HOGENOMi | CLU_066458_1_0_6 |
InParanoidi | Q47319 |
OMAi | WQPYVVF |
PhylomeDBi | Q47319 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7349-MONOMER |
Miscellaneous databases
PROi | PR:Q47319 |
Family and domain databases
InterProi | View protein in InterPro IPR005636, DTW IPR039262, DTWD2/YfiP |
PANTHERi | PTHR21392, PTHR21392, 1 hit |
Pfami | View protein in Pfam PF03942, DTW, 1 hit |
SMARTi | View protein in SMART SM01144, DTW, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TAPT_ECOLI | |
Accessioni | Q47319Primary (citable) accession number: Q47319 Secondary accession number(s): Q2MAF3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 15, 1998 | |
Last modified: | May 25, 2022 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families