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UniProtKB - Q47258 (HLYB3_ECOLX)
Protein
Alpha-hemolysin translocation ATP-binding protein HlyB
Gene
hlyB
Organism
Escherichia coli
Status
Functioni
Part of the ABC transporter complex HlyBD involved in hemolysin export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity).
By similarityMiscellaneous
The complex HlyBD-TolC (OMF) forms a single transport channel across the two membranes, allowing direct export of alpha-hemolysin. These channel is involved in type 1 secretion system (By similarity).By similarity
Caution
Tyr-9 is present instead of the conserved Cys which is expected to be the active site residue of peptidase C39. Thus they are presumed to be without peptidase activity.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 83 | PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 502 – 509 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ABC-type transporter activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- peptidase activity Source: InterPro
GO - Biological processi
- protein secretion by the type I secretion system Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Biological process | Transport |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-hemolysin translocation ATP-binding protein HlyB |
Gene namesi | Name:hlyB |
Organismi | Escherichia coli |
Taxonomic identifieri | 562 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia |
Subcellular locationi
Plasma membrane
- Cell inner membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- type I protein secretion system complex Source: InterPro
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 158 – 178 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 191 – 211 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 269 – 289 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 295 – 315 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 388 – 408 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000092373 | 1 – 707 | Alpha-hemolysin translocation ATP-binding protein HlyBAdd BLAST | 707 |
Interactioni
Subunit structurei
Homodimer.
By similarityStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q47258 |
BMRBi | Q47258 |
SMRi | Q47258 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 125 | Peptidase C39PROSITE-ProRule annotationAdd BLAST | 123 | |
Domaini | 154 – 436 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 283 | |
Domaini | 468 – 703 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 236 |
Domaini
In HlyB the peptidase C39 domain, the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.
Sequence similaritiesi
Belongs to the ABC transporter superfamily. Protein-1 exporter (TC 3.A.1.109) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
OMAi | ERQRMTI |
Family and domain databases
CDDi | cd02417, Peptidase_C39_likeA, 1 hit |
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR010132, ATPase_T1SS_HlyB IPR027417, P-loop_NTPase IPR005074, Peptidase_C39 IPR039395, Peptidase_C39-like_A IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit PF03412, Peptidase_C39, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR01846, type_I_sec_HlyB, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit PS50990, PEPTIDASE_C39, 1 hit |
i Sequence
Sequence statusi: Complete.
Q47258-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDSCHKIDYG LYALEILAQY HNVSVNPEEI KHRFDTDGTG LGLTSWLLAA
60 70 80 90 100
KSLELKVKQV KKTIDRLNFI SLPALVWRED GRHFILTKVS KEANRYLIFD
110 120 130 140 150
LEQRNPRVLE QSEFEALYQG HIILIASRSS VAGKLAKFDF TWFIPAIIKY
160 170 180 190 200
RRIFIETLVV SVFLQLFALI TPLFFQVVMD KVLVHRGFST LNVITVALSV
210 220 230 240 250
VVVFEIILSG LRTYIFAHST SRIDVELGAK LFRHLLALPI SYFESRRVGD
260 270 280 290 300
TVARVRELDQ IRNFLTGQAL TSVLDLLFSF IFFAVMWYYS PKLTLVILFS
310 320 330 340 350
LPCYAAWSVF ISPILRRRLD DKFSRNADNQ SFLVESVTAI NTIKAMAVSP
360 370 380 390 400
QMTNIWDKQL AGYVAAGFKV TVLATIGQQG IQLIQKTVMI INLWLGAHLV
410 420 430 440 450
ISGDLSIGQL IAFNMLAGQI VAPVIRLAQI WQDFQQVGIS VTRLGDVLNS
460 470 480 490 500
PTESYHGKLA LPEINGDITF RNIRFRYKPD SPVILDNINL SIKQGEVIGI
510 520 530 540 550
VGRSGSGKST LTKLIQRFYI PENGQVLIDG HDLALADPNW LRRQVGVVLQ
560 570 580 590 600
DNVLLNRSII DNISLANPGM SVEKVIYAAK LAGAHDFISE LREGYNTIVG
610 620 630 640 650
EQGAGLSGGQ RQRIAIARAL VNNPKILIFD EATSALDYES EHIIMRNMHK
660 670 680 690 700
ICKGRTVIII AHRLSTVKNA DRIIVMEKGK IVEQGKHKEL LSEPESLYSY
LYQLQSD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81823 Genomic DNA Translation: AAA23978.1 |
PIRi | A42255, LEECB |
RefSeqi | WP_000376543.1, NZ_WIKQ01000030.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81823 Genomic DNA Translation: AAA23978.1 |
PIRi | A42255, LEECB |
RefSeqi | WP_000376543.1, NZ_WIKQ01000030.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3ZUA | NMR | - | A | 2-137 | [»] | |
AlphaFoldDBi | Q47258 | |||||
BMRBi | Q47258 | |||||
SMRi | Q47258 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Phylogenomic databases
OMAi | ERQRMTI |
Family and domain databases
CDDi | cd02417, Peptidase_C39_likeA, 1 hit |
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR010132, ATPase_T1SS_HlyB IPR027417, P-loop_NTPase IPR005074, Peptidase_C39 IPR039395, Peptidase_C39-like_A IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit PF03412, Peptidase_C39, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR01846, type_I_sec_HlyB, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit PS50990, PEPTIDASE_C39, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HLYB3_ECOLX | |
Accessioni | Q47258Primary (citable) accession number: Q47258 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families