UniProtKB - Q47155 (DPO4_ECOLI)
DNA polymerase IV
dinB
Functioni
Poorly processive, error-prone DNA polymerase involved in translesion repair and untargeted mutagenesis (PubMed:10488344, PubMed:10801133).
Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by Pol IV. Exhibits no 3'-5' exonuclease (proofreading) activity (PubMed:10488344).
Overexpression of Pol IV results in increased frameshift mutagenesis. It is required for stationary-phase adaptive mutation, which provides the bacterium with flexibility in dealing with environmental stress, enhancing long-term survival and evolutionary fitness. Not seen to be involved in translesion snythesis even when stimulated by the beta slding-clamp and clamp-loading complex, which do however increase non-targeted DNA polymerase efficiency 3,000-fold, may be due to targeting to stalled replication forks on nondamaged DNA (PubMed:10801133, PubMed:16168375).
Involved in translesional synthesis, in conjunction with the beta clamp from PolIII (PubMed:14592985, PubMed:14729336).
1 Publication9 PublicationsCatalytic activityi
- EC:2.7.7.7
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 8 | MagnesiumBy similarity | 1 | |
Sitei | 13 | Substrate discriminationBy similarity | 1 | |
Metal bindingi | 103 | MagnesiumBy similarity | 1 | |
Active sitei | 104 | By similarity | 1 |
GO - Molecular functioni
- damaged DNA binding Source: InterPro
- DNA-directed DNA polymerase activity Source: EcoCyc
- magnesium ion binding Source: EcoCyc
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- DNA-dependent DNA replication Source: UniProtKB-UniRule
- DNA synthesis involved in DNA repair Source: EcoliWiki
- error-free translesion synthesis Source: EcoCyc
- error-prone translesion synthesis Source: EcoCyc
- SOS response Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6115-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase IV (EC:2.7.7.7)Short name: Pol IV Alternative name(s): Translesion synthesis polymerase IV1 Publication Short name: TSL polymerase IV |
Gene namesi | Name:dinB Synonyms:dinP Ordered Locus Names:b0231, JW0221 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: EcoliWiki
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 8 | D → A or H: Loss of function. 1 Publication | 1 | |
Mutagenesisi | 49 | R → A or F: Loss of function. 1 Publication | 1 | |
Mutagenesisi | 103 | D → A or N: Loss of function. 1 Publication | 1 | |
Mutagenesisi | 104 | E → A: Loss of function. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173912 | 1 – 351 | DNA polymerase IVAdd BLAST | 351 |
Proteomic databases
PaxDbi | Q47155 |
PRIDEi | Q47155 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Binary interactionsi
Protein-protein interaction databases
BioGRIDi | 4261678, 55 interactors |
IntActi | Q47155, 3 interactors |
MINTi | Q47155 |
STRINGi | 511145.b0231 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q47155 |
SMRi | Q47155 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q47155 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 185 | UmuCAdd BLAST | 182 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0389, Bacteria |
HOGENOMi | CLU_012348_1_2_6 |
InParanoidi | Q47155 |
OMAi | CKPDGLL |
PhylomeDBi | Q47155 |
Family and domain databases
CDDi | cd03586, PolY_Pol_IV_kappa, 1 hit |
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit |
HAMAPi | MF_01113, DNApol_IV, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR017961, DNA_pol_Y-fam_little_finger IPR022880, DNApol_IV IPR024728, PolY_HhH_motif IPR043128, Rev_trsase/Diguanyl_cyclase IPR001126, UmuC |
Pfami | View protein in Pfam PF00817, IMS, 1 hit PF11799, IMS_C, 1 hit PF11798, IMS_HHH, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50173, UMUC, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MRKIIHVDMD CFFAAVEMRD NPALRDIPIA IGGSRERRGV ISTANYPARK
60 70 80 90 100
FGVRSAMPTG MALKLCPHLT LLPGRFDAYK EASNHIREIF SRYTSRIEPL
110 120 130 140 150
SLDEAYLDVT DSVHCHGSAT LIAQEIRQTI FNELQLTASA GVAPVKFLAK
160 170 180 190 200
IASDMNKPNG QFVITPAEVP AFLQTLPLAK IPGVGKVSAA KLEAMGLRTC
210 220 230 240 250
GDVQKCDLVM LLKRFGKFGR ILWERSQGID ERDVNSERLR KSVGVERTMA
260 270 280 290 300
EDIHHWSECE AIIERLYPEL ERRLAKVKPD LLIARQGVKL KFDDFQQTTQ
310 320 330 340 350
EHVWPRLNKA DLIATARKTW DERRGGRGVR LVGLHVTLLD PQMERQLVLG
L
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 36 – 38 | ERR → ARG in strain: ECOR 45B1. | 3 | |
Natural varianti | 124 | Q → K in strain: ECOR 35D. | 1 | |
Natural varianti | 132 | N → S in strain: ECOR 34B1 and ECOR 37UG. | 1 | |
Natural varianti | 135 | Q → H in strain: ECOR 70B1. | 1 | |
Natural varianti | 170 | P → S in strain: ECOR 37UG. | 1 | |
Natural varianti | 171 | A → T in strain: ECOR 45B1, ECOR 46D, ECOR 49D and ECOR 50D. | 1 | |
Natural varianti | 176 | L → F in strain: ECOR 37UG. | 1 | |
Natural varianti | 201 | G → S in strain: ECOR 59B2. | 1 | |
Natural varianti | 210 | M → I in strain: ECOR 37UG, ECOR 45B1, ECOR 51B2, ECOR 52B2, ECOR 58B1 and ECOR 70B1. | 1 | |
Natural varianti | 210 | M → T in strain: ECOR 35D, ECOR 46D, ECOR 49D, ECOR 50D, ECOR 57B2, ECOR 59B2, ECOR 60B2 and ECOR 62B2. | 1 | |
Natural varianti | 225 | R → C in strain: ECOR 59B2 and ECOR 60B2. | 1 | |
Natural varianti | 310 | A → S in strain: ECOR 57B2, ECOR 59B2, ECOR 60B2 and ECOR 62B2. | 1 | |
Natural varianti | 321 | D → N in strain: ECOR 35D. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D38582 Genomic DNA Translation: BAA07593.1 U70214 Genomic DNA Translation: AAB08651.1 U00096 Genomic DNA Translation: AAC73335.1 AP009048 Genomic DNA Translation: BAA77901.1 AF483080 Genomic DNA Translation: AAL91943.1 AF483081 Genomic DNA Translation: AAL91944.1 AF483082 Genomic DNA Translation: AAL91945.1 AF483083 Genomic DNA Translation: AAL91946.1 AF483084 Genomic DNA Translation: AAL91947.1 AF483085 Genomic DNA Translation: AAL91948.1 AF483086 Genomic DNA Translation: AAL91949.1 AF483087 Genomic DNA Translation: AAL91950.1 AF483088 Genomic DNA Translation: AAL91951.1 AF483089 Genomic DNA Translation: AAL91952.1 AF483090 Genomic DNA Translation: AAL91953.1 AF483091 Genomic DNA Translation: AAL91954.1 AF483092 Genomic DNA Translation: AAL91955.1 AF483093 Genomic DNA Translation: AAL91956.1 AF483094 Genomic DNA Translation: AAL91957.1 AF483095 Genomic DNA Translation: AAL91958.1 AF483096 Genomic DNA Translation: AAL91959.1 AF483097 Genomic DNA Translation: AAL91960.1 AF483098 Genomic DNA Translation: AAL91961.1 AF483099 Genomic DNA Translation: AAL91962.1 AF483100 Genomic DNA Translation: AAL91963.1 AF483101 Genomic DNA Translation: AAL91964.1 AF483102 Genomic DNA Translation: AAL91965.1 AF483103 Genomic DNA Translation: AAL91966.1 AF483104 Genomic DNA Translation: AAL91967.1 AF483105 Genomic DNA Translation: AAL91968.1 |
PIRi | H64747 |
RefSeqi | NP_414766.1, NC_000913.3 WP_001226164.1, NZ_SSZK01000050.1 |
Genome annotation databases
EnsemblBacteriai | AAC73335; AAC73335; b0231 BAA77901; BAA77901; BAA77901 |
GeneIDi | 944922 |
KEGGi | ecj:JW0221 eco:b0231 |
PATRICi | fig|1411691.4.peg.2052 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D38582 Genomic DNA Translation: BAA07593.1 U70214 Genomic DNA Translation: AAB08651.1 U00096 Genomic DNA Translation: AAC73335.1 AP009048 Genomic DNA Translation: BAA77901.1 AF483080 Genomic DNA Translation: AAL91943.1 AF483081 Genomic DNA Translation: AAL91944.1 AF483082 Genomic DNA Translation: AAL91945.1 AF483083 Genomic DNA Translation: AAL91946.1 AF483084 Genomic DNA Translation: AAL91947.1 AF483085 Genomic DNA Translation: AAL91948.1 AF483086 Genomic DNA Translation: AAL91949.1 AF483087 Genomic DNA Translation: AAL91950.1 AF483088 Genomic DNA Translation: AAL91951.1 AF483089 Genomic DNA Translation: AAL91952.1 AF483090 Genomic DNA Translation: AAL91953.1 AF483091 Genomic DNA Translation: AAL91954.1 AF483092 Genomic DNA Translation: AAL91955.1 AF483093 Genomic DNA Translation: AAL91956.1 AF483094 Genomic DNA Translation: AAL91957.1 AF483095 Genomic DNA Translation: AAL91958.1 AF483096 Genomic DNA Translation: AAL91959.1 AF483097 Genomic DNA Translation: AAL91960.1 AF483098 Genomic DNA Translation: AAL91961.1 AF483099 Genomic DNA Translation: AAL91962.1 AF483100 Genomic DNA Translation: AAL91963.1 AF483101 Genomic DNA Translation: AAL91964.1 AF483102 Genomic DNA Translation: AAL91965.1 AF483103 Genomic DNA Translation: AAL91966.1 AF483104 Genomic DNA Translation: AAL91967.1 AF483105 Genomic DNA Translation: AAL91968.1 |
PIRi | H64747 |
RefSeqi | NP_414766.1, NC_000913.3 WP_001226164.1, NZ_SSZK01000050.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OK7 | X-ray | 1.65 | C | 336-351 | [»] | |
1UNN | X-ray | 1.90 | C/D | 243-351 | [»] | |
4IR1 | X-ray | 2.38 | A/F | 2-351 | [»] | |
4IR9 | X-ray | 2.33 | A/F | 2-351 | [»] | |
4IRC | X-ray | 2.67 | A/F | 2-341 | [»] | |
4IRD | X-ray | 2.48 | A/F | 2-341 | [»] | |
4IRK | X-ray | 2.32 | A/B | 2-341 | [»] | |
4Q43 | X-ray | 2.45 | A/F | 2-351 | [»] | |
4Q44 | X-ray | 2.71 | A/F | 2-341 | [»] | |
4Q45 | X-ray | 2.18 | A/F | 2-341 | [»] | |
4R8U | X-ray | 2.30 | A | 2-340 | [»] | |
B | 2-338 | [»] | ||||
5C5J | X-ray | 2.10 | A/F | 2-351 | [»] | |
5YUR | X-ray | 2.04 | A/F | 2-351 | [»] | |
5YUS | X-ray | 1.94 | A/F | 2-351 | [»] | |
5YUT | X-ray | 2.15 | A/F | 2-351 | [»] | |
5YUU | X-ray | 1.89 | A/F | 2-351 | [»] | |
5YUV | X-ray | 2.06 | A/F | 2-351 | [»] | |
5YUW | X-ray | 2.12 | A/F | 2-351 | [»] | |
5YUX | X-ray | 2.04 | A/F | 2-351 | [»] | |
5YUY | X-ray | 1.74 | A/F | 2-351 | [»] | |
5YUZ | X-ray | 1.83 | A/F | 2-351 | [»] | |
5YV0 | X-ray | 2.09 | A/F | 2-351 | [»] | |
5YV1 | X-ray | 2.09 | A/F | 2-351 | [»] | |
5YV2 | X-ray | 1.90 | A/F | 2-351 | [»] | |
5YV3 | X-ray | 2.03 | A/F | 2-351 | [»] | |
5YYD | X-ray | 2.05 | A/F | 2-351 | [»] | |
5YYE | X-ray | 2.33 | A/F | 2-351 | [»] | |
5ZLV | X-ray | 2.35 | A/F | 2-351 | [»] | |
6IG1 | X-ray | 1.97 | A/F | 2-351 | [»] | |
6JUP | X-ray | 2.44 | A/F | 2-341 | [»] | |
6JUQ | X-ray | 2.74 | A/F | 2-341 | [»] | |
AlphaFoldDBi | Q47155 | |||||
SMRi | Q47155 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261678, 55 interactors |
IntActi | Q47155, 3 interactors |
MINTi | Q47155 |
STRINGi | 511145.b0231 |
Proteomic databases
PaxDbi | Q47155 |
PRIDEi | Q47155 |
Genome annotation databases
EnsemblBacteriai | AAC73335; AAC73335; b0231 BAA77901; BAA77901; BAA77901 |
GeneIDi | 944922 |
KEGGi | ecj:JW0221 eco:b0231 |
PATRICi | fig|1411691.4.peg.2052 |
Organism-specific databases
EchoBASEi | EB2935 |
Phylogenomic databases
eggNOGi | COG0389, Bacteria |
HOGENOMi | CLU_012348_1_2_6 |
InParanoidi | Q47155 |
OMAi | CKPDGLL |
PhylomeDBi | Q47155 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6115-MONOMER |
Miscellaneous databases
EvolutionaryTracei | Q47155 |
PROi | PR:Q47155 |
Family and domain databases
CDDi | cd03586, PolY_Pol_IV_kappa, 1 hit |
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit |
HAMAPi | MF_01113, DNApol_IV, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR017961, DNA_pol_Y-fam_little_finger IPR022880, DNApol_IV IPR024728, PolY_HhH_motif IPR043128, Rev_trsase/Diguanyl_cyclase IPR001126, UmuC |
Pfami | View protein in Pfam PF00817, IMS, 1 hit PF11799, IMS_C, 1 hit PF11798, IMS_HHH, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50173, UMUC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPO4_ECOLI | |
Accessioni | Q47155Primary (citable) accession number: Q47155 Secondary accession number(s): Q47683 Q8RNJ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families