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UniProtKB - Q47146 (FADE_ECOLI)
Protein
Acyl-coenzyme A dehydrogenase
Gene
fadE
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the dehydrogenation of acyl-coenzymes A (acyl-CoAs) to 2-enoyl-CoAs, the first step of the beta-oxidation cycle of fatty acid degradation. Is required for E.coli to utilize dodecanoate or oleate as the sole carbon and energy source for growth.
1 PublicationCaution
Was originally named fadF.Curated
Catalytic activityi
- a medium-chain 2,3-saturated fatty acyl-CoA + H+ + oxidized [electron-transfer flavoprotein] = a medium-chain trans-(2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]1 PublicationEC:1.3.8.71 Publication
- a long-chain 2,3-saturated fatty acyl-CoA + H+ + oxidized [electron-transfer flavoprotein] = a long-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]1 PublicationEC:1.3.8.81 Publication
Cofactori
FADBy similarity
: fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 497 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- acyl-CoA dehydrogenase activity Source: EcoCyc
- flavin adenine dinucleotide binding Source: InterPro
- long-chain-acyl-CoA dehydrogenase activity Source: UniProtKB-EC
GO - Biological processi
- fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | EcoCyc:ACYLCOADEHYDROG-MONOMER |
UniPathwayi | UPA00659 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:fadE1 Publication Synonyms:yafH Ordered Locus Names:b0221, JW5020 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene are unable to grow on octanoate, decanoate, dodecanoate, or oleate as the sole carbon and energy source, but grow well on acetate, while the wild-type strain can grow on acetate, dodecanoate, and oleate.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201200 | 1 – 814 | Acyl-coenzyme A dehydrogenaseAdd BLAST | 814 |
Proteomic databases
jPOSTi | Q47146 |
PaxDbi | Q47146 |
PRIDEi | Q47146 |
Expressioni
Inductioni
Transcription of fadE is negatively regulated by FadR. Induced in the presence of fatty acids, in a FadR-dependent manner.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259770, 205 interactors |
STRINGi | 511145.b0221 |
Family & Domainsi
Sequence similaritiesi
Belongs to the acyl-CoA dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | COG1960, Bacteria |
HOGENOMi | CLU_012192_0_0_6 |
InParanoidi | Q47146 |
PhylomeDBi | Q47146 |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
InterProi | View protein in InterPro IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR013786, AcylCoA_DH/ox_N IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom IPR015396, DUF1974 |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF02771, Acyl-CoA_dh_N, 1 hit PF09317, DUF1974, 1 hit |
SUPFAMi | SSF47203, SSF47203, 1 hit SSF56645, SSF56645, 1 hit |
i Sequence
Sequence statusi: Complete.
Q47146-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMILSILATV VLLGALFYHR VSLFISSLIL LAWTAALGVA GLWSAWVLVP
60 70 80 90 100
LAIILVPFNF APMRKSMISA PVFRGFRKVM PPMSRTEKEA IDAGTTWWEG
110 120 130 140 150
DLFQGKPDWK KLHNYPQPRL TAEEQAFLDG PVEEACRMAN DFQITHELAD
160 170 180 190 200
LPPELWAYLK EHRFFAMIIK KEYGGLEFSA YAQSRVLQKL SGVSGILAIT
210 220 230 240 250
VGVPNSLGPG ELLQHYGTDE QKDHYLPRLA RGQEIPCFAL TSPEAGSDAG
260 270 280 290 300
AIPDTGIVCM GEWQGQQVLG MRLTWNKRYI TLAPIATVLG LAFKLSDPEK
310 320 330 340 350
LLGGAEDLGI TCALIPTTTP GVEIGRRHFP LNVPFQNGPT RGKDVFVPID
360 370 380 390 400
YIIGGPKMAG QGWRMLVECL SVGRGITLPS NSTGGVKSVA LATGAYAHIR
410 420 430 440 450
RQFKISIGKM EGIEEPLARI AGNAYVMDAA ASLITYGIML GEKPAVLSAI
460 470 480 490 500
VKYHCTHRGQ QSIIDAMDIT GGKGIMLGQS NFLARAYQGA PIAITVEGAN
510 520 530 540 550
ILTRSMMIFG QGAIRCHPYV LEEMEAAKNN DVNAFDKLLF KHIGHVGSNK
560 570 580 590 600
VRSFWLGLTR GLTSSTPTGD ATKRYYQHLN RLSANLALLS DVSMAVLGGS
610 620 630 640 650
LKRRERISAR LGDILSQLYL ASAVLKRYDD EGRNEADLPL VHWGVQDALY
660 670 680 690 700
QAEQAMDDLL QNFPNRVVAG LLNVVIFPTG RHYLAPSDKL DHKVAKILQV
710 720 730 740 750
PNATRSRIGR GQYLTPSEHN PVGLLEEALV DVIAADPIHQ RICKELGKNL
760 770 780 790 800
PFTRLDELAH NALVKGLIDK DEAAILVKAE ESRLRSINVD DFDPEELATK
810
PVKLPEKVRK VEAA
Sequence cautioni
The sequence BAA07583 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U70214 Genomic DNA Translation: AAB08643.1 U00096 Genomic DNA Translation: AAC73325.2 AP009048 Genomic DNA Translation: BAA77891.2 D38582 Genomic DNA Translation: BAA07583.1 Different initiation. |
PIRi | F64746 |
RefSeqi | NP_414756.2, NC_000913.3 WP_000973083.1, NZ_SSZK01000029.1 |
Genome annotation databases
EnsemblBacteriai | AAC73325; AAC73325; b0221 BAA77891; BAA77891; BAA77891 |
GeneIDi | 949007 |
KEGGi | ecj:JW5020 eco:b0221 |
PATRICi | fig|1411691.4.peg.2062 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U70214 Genomic DNA Translation: AAB08643.1 U00096 Genomic DNA Translation: AAC73325.2 AP009048 Genomic DNA Translation: BAA77891.2 D38582 Genomic DNA Translation: BAA07583.1 Different initiation. |
PIRi | F64746 |
RefSeqi | NP_414756.2, NC_000913.3 WP_000973083.1, NZ_SSZK01000029.1 |
3D structure databases
SMRi | Q47146 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259770, 205 interactors |
STRINGi | 511145.b0221 |
Proteomic databases
jPOSTi | Q47146 |
PaxDbi | Q47146 |
PRIDEi | Q47146 |
Genome annotation databases
EnsemblBacteriai | AAC73325; AAC73325; b0221 BAA77891; BAA77891; BAA77891 |
GeneIDi | 949007 |
KEGGi | ecj:JW5020 eco:b0221 |
PATRICi | fig|1411691.4.peg.2062 |
Organism-specific databases
EchoBASEi | EB2939 |
Phylogenomic databases
eggNOGi | COG1960, Bacteria |
HOGENOMi | CLU_012192_0_0_6 |
InParanoidi | Q47146 |
PhylomeDBi | Q47146 |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
BioCyci | EcoCyc:ACYLCOADEHYDROG-MONOMER |
Miscellaneous databases
PROi | PR:Q47146 |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
InterProi | View protein in InterPro IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR013786, AcylCoA_DH/ox_N IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom IPR015396, DUF1974 |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF02771, Acyl-CoA_dh_N, 1 hit PF09317, DUF1974, 1 hit |
SUPFAMi | SSF47203, SSF47203, 1 hit SSF56645, SSF56645, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FADE_ECOLI | |
Accessioni | Q47146Primary (citable) accession number: Q47146 Secondary accession number(s): P71283, P75673, Q47680 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families