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UniProtKB - Q47146 (FADE_ECOLI)

Entry version 147 (02 Dec 2020)
Sequence version 2 (01 Nov 2002)
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Protein

Acyl-coenzyme A dehydrogenase

Gene

fadE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at transcript leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the dehydrogenation of acyl-coenzymes A (acyl-CoAs) to 2-enoyl-CoAs, the first step of the beta-oxidation cycle of fatty acid degradation. Is required for E.coli to utilize dodecanoate or oleate as the sole carbon and energy source for growth.1 Publication

Caution

Was originally named fadF.Curated

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

FADBy similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei497Proton acceptorBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid metabolism, Lipid metabolism
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:ACYLCOADEHYDROG-MONOMER
MetaCyc:ACYLCOADEHYDROG-MONOMER

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00659

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Acyl-coenzyme A dehydrogenase1 Publication (EC:1.3.8.71 Publication, EC:1.3.8.81 Publication)
Short name:
ACDH
Short name:
Acyl-CoA dehydrogenase1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:fadE1 Publication
Synonyms:yafH
Ordered Locus Names:b0221, JW5020
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Cells lacking this gene are unable to grow on octanoate, decanoate, dodecanoate, or oleate as the sole carbon and energy source, but grow well on acetate, while the wild-type strain can grow on acetate, dodecanoate, and oleate.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002012001 – 814Acyl-coenzyme A dehydrogenaseAdd BLAST814

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q47146

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q47146

PRoteomics IDEntifications database

More...PRIDEi		Q47146

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Transcription of fadE is negatively regulated by FadR. Induced in the presence of fatty acids, in a FadR-dependent manner.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4259770, 205 interactors

STRING: functional protein association networks

More...STRINGi		511145.b0221

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG1960, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_012192_0_0_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q47146

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q47146

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.540.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR006091, Acyl-CoA_Oxase/DH_cen-dom
IPR036250, AcylCo_DH-like_C
IPR009075, AcylCo_DH/oxidase_C
IPR013786, AcylCoA_DH/ox_N
IPR037069, AcylCoA_DH/ox_N_sf
IPR009100, AcylCoA_DH/oxidase_NM_dom
IPR015396, DUF1974

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00441, Acyl-CoA_dh_1, 1 hit
PF02770, Acyl-CoA_dh_M, 1 hit
PF02771, Acyl-CoA_dh_N, 1 hit
PF09317, DUF1974, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF47203, SSF47203, 1 hit
SSF56645, SSF56645, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q47146-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMILSILATV VLLGALFYHR VSLFISSLIL LAWTAALGVA GLWSAWVLVP 
        60         70         80         90        100
LAIILVPFNF APMRKSMISA PVFRGFRKVM PPMSRTEKEA IDAGTTWWEG 
       110        120        130        140        150
DLFQGKPDWK KLHNYPQPRL TAEEQAFLDG PVEEACRMAN DFQITHELAD 
       160        170        180        190        200
LPPELWAYLK EHRFFAMIIK KEYGGLEFSA YAQSRVLQKL SGVSGILAIT 
       210        220        230        240        250
VGVPNSLGPG ELLQHYGTDE QKDHYLPRLA RGQEIPCFAL TSPEAGSDAG 
       260        270        280        290        300
AIPDTGIVCM GEWQGQQVLG MRLTWNKRYI TLAPIATVLG LAFKLSDPEK 
       310        320        330        340        350
LLGGAEDLGI TCALIPTTTP GVEIGRRHFP LNVPFQNGPT RGKDVFVPID 
       360        370        380        390        400
YIIGGPKMAG QGWRMLVECL SVGRGITLPS NSTGGVKSVA LATGAYAHIR 
       410        420        430        440        450
RQFKISIGKM EGIEEPLARI AGNAYVMDAA ASLITYGIML GEKPAVLSAI 
       460        470        480        490        500
VKYHCTHRGQ QSIIDAMDIT GGKGIMLGQS NFLARAYQGA PIAITVEGAN 
       510        520        530        540        550
ILTRSMMIFG QGAIRCHPYV LEEMEAAKNN DVNAFDKLLF KHIGHVGSNK 
       560        570        580        590        600
VRSFWLGLTR GLTSSTPTGD ATKRYYQHLN RLSANLALLS DVSMAVLGGS 
       610        620        630        640        650
LKRRERISAR LGDILSQLYL ASAVLKRYDD EGRNEADLPL VHWGVQDALY 
       660        670        680        690        700
QAEQAMDDLL QNFPNRVVAG LLNVVIFPTG RHYLAPSDKL DHKVAKILQV 
       710        720        730        740        750
PNATRSRIGR GQYLTPSEHN PVGLLEEALV DVIAADPIHQ RICKELGKNL 
       760        770        780        790        800
PFTRLDELAH NALVKGLIDK DEAAILVKAE ESRLRSINVD DFDPEELATK 
       810 
PVKLPEKVRK VEAA
Length:814
Mass (Da):89,224
Last modified:November 1, 2002 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iAD9E40ACB44CF781
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAA07583 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U70214 Genomic DNA Translation: AAB08643.1
U00096 Genomic DNA Translation: AAC73325.2
AP009048 Genomic DNA Translation: BAA77891.2
D38582 Genomic DNA Translation: BAA07583.1 Different initiation.

Protein sequence database of the Protein Information Resource

More...PIRi		F64746

NCBI Reference Sequences

More...RefSeqi		NP_414756.2, NC_000913.3
WP_000973083.1, NZ_SSZK01000029.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC73325; AAC73325; b0221
BAA77891; BAA77891; BAA77891

Database of genes from NCBI RefSeq genomes

More...GeneIDi		949007

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW5020
eco:b0221

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.2062

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA Translation: AAB08643.1
U00096 Genomic DNA Translation: AAC73325.2
AP009048 Genomic DNA Translation: BAA77891.2
D38582 Genomic DNA Translation: BAA07583.1 Different initiation.
PIRiF64746
RefSeqiNP_414756.2, NC_000913.3
WP_000973083.1, NZ_SSZK01000029.1

3D structure databases

Database of comparative protein structure models

More...ModBasei		Search...

SWISS-MODEL Interactive Workspace

More...SWISS-MODEL-Workspacei		Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi4259770, 205 interactors
STRINGi511145.b0221

Proteomic databases

jPOSTiQ47146
PaxDbiQ47146
PRIDEiQ47146

Genome annotation databases

EnsemblBacteriaiAAC73325; AAC73325; b0221
BAA77891; BAA77891; BAA77891
GeneIDi949007
KEGGiecj:JW5020
eco:b0221
PATRICifig|1411691.4.peg.2062

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB2939

Phylogenomic databases

eggNOGiCOG1960, Bacteria
HOGENOMiCLU_012192_0_0_6
InParanoidiQ47146
PhylomeDBiQ47146

Enzyme and pathway databases

UniPathwayiUPA00659
BioCyciEcoCyc:ACYLCOADEHYDROG-MONOMER
MetaCyc:ACYLCOADEHYDROG-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:Q47146

Family and domain databases

Gene3Di1.10.540.10, 1 hit
InterProiView protein in InterPro
IPR006091, Acyl-CoA_Oxase/DH_cen-dom
IPR036250, AcylCo_DH-like_C
IPR009075, AcylCo_DH/oxidase_C
IPR013786, AcylCoA_DH/ox_N
IPR037069, AcylCoA_DH/ox_N_sf
IPR009100, AcylCoA_DH/oxidase_NM_dom
IPR015396, DUF1974
PfamiView protein in Pfam
PF00441, Acyl-CoA_dh_1, 1 hit
PF02770, Acyl-CoA_dh_M, 1 hit
PF02771, Acyl-CoA_dh_N, 1 hit
PF09317, DUF1974, 1 hit
SUPFAMiSSF47203, SSF47203, 1 hit
SSF56645, SSF56645, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiFADE_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q47146
Secondary accession number(s): P71283, P75673, Q47680
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 2002
Last modified: December 2, 2020
This is version 147 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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