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Protein

Hca operon transcriptional activator HcaR

Gene

hcaR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator of the hca operon for 3-phenylpropionic acid catabolism.

Caution

The 21 C-terminal amino acids have been proposed to be expressed from an independent promoter within this gene and called IroK. Overexpression of IroK has been suggested to be responsible for 3-hydroxypropionic acid (3-HP) resistance. However the IroK peptide's existence has not been proven, nor has mutagenesis of HcaR itself rather than overexpression of IroK been ruled out as the cause of 3-HP resistance (PubMed:22161628).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi18 – 38H-T-H motifPROSITE-ProRule annotationAdd BLAST21

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • DNA binding transcription factor activity Source: EcoCyc

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G7331-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Hca operon transcriptional activator HcaR
Gene namesi
Name:hcaR
Synonyms:phdR, yfhT
Ordered Locus Names:b2537, JW2521
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13455 hcaR

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001056311 – 296Hca operon transcriptional activator HcaRAdd BLAST296

Proteomic databases

PaxDbiQ47141
PRIDEiQ47141

Interactioni

Protein-protein interaction databases

BioGridi4261574, 17 interactors
IntActiQ47141, 2 interactors
STRINGi316385.ECDH10B_2704

Structurei

3D structure databases

ProteinModelPortaliQ47141
SMRiQ47141
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 58HTH lysR-typePROSITE-ProRule annotationAdd BLAST58

Phylogenomic databases

eggNOGiENOG4108W5M Bacteria
ENOG4111IYC LUCA
HOGENOMiHOG000233514
InParanoidiQ47141
KOiK05817
OMAiKGIQQNM
PhylomeDBiQ47141

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR005119 LysR_subst-bd
IPR000847 Tscrpt_reg_HTH_LysR
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00126 HTH_1, 1 hit
PF03466 LysR_substrate, 1 hit
PRINTSiPR00039 HTHLYSR
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50931 HTH_LYSR, 1 hit

Sequencei

Sequence statusi: Complete.

Q47141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELRHLRYFV AVAQALNFTR AAEKLHTSQP SLSSQIRDLE NCVGVPLLVR
60 70 80 90 100
DKRKVALTAA GECFLQDALA ILEQAENAKL RARKIVQEDR QLTIGFVPSA
110 120 130 140 150
EVNLLPKVLP MFRLRQPDTL IELVSLITTQ QEEKIRRGEL DVGLMRHPVY
160 170 180 190 200
SPEIDYLELF DEPLVVVLPV DHPLAHEKEI TAAQLDGVNF VSTDPVYSGS
210 220 230 240 250
LAPIVKAWFA QENSQPNIVQ VATNILVTMN LVGMGLGVTL IPGYMNNFNT
260 270 280 290
GQVVFRPIAG NVPSIALLMA WKKGEMKPAL RDFIAIVQER LASVTA
Length:296
Mass (Da):32,838
Last modified:November 1, 1997 - v2
Checksum:i72FF08A62D490AAA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127 – 141ITTQQ…RGELD → SPRNRRKKFAVVNST in CAA86020 (Ref. 1) CuratedAdd BLAST15
Sequence conflicti145 – 148MRHP → NCAIH in CAA86020 (Ref. 1) Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37966 Genomic DNA Translation: CAA86020.1
U00096 Genomic DNA Translation: AAC75590.1
AP009048 Genomic DNA Translation: BAA16432.2
PIRiH65030
RefSeqiNP_417032.1, NC_000913.3
WP_000423261.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75590; AAC75590; b2537
BAA16432; BAA16432; BAA16432
GeneIDi947000
KEGGiecj:JW2521
eco:b2537
PATRICifig|1411691.4.peg.4197

Entry informationi

Entry nameiHCAR_ECOLI
AccessioniPrimary (citable) accession number: Q47141
Secondary accession number(s): P76583, P77167
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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