UniProtKB - Q47141 (HCAR_ECOLI)
Protein
Hca operon transcriptional activator HcaR
Gene
hcaR
Organism
Escherichia coli (strain K12)
Status
Functioni
Transcriptional activator of the hca operon for 3-phenylpropionic acid catabolism.
Caution
The 21 C-terminal amino acids have been proposed to be expressed from an independent promoter within this gene and called IroK. Overexpression of IroK has been suggested to be responsible for 3-hydroxypropionic acid (3-HP) resistance. However the IroK peptide's existence has not been proven, nor has mutagenesis of HcaR itself rather than overexpression of IroK been ruled out as the cause of 3-HP resistance (PubMed:22161628).1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 18 – 38 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 21 |
GO - Molecular functioni
- bacterial-type cis-regulatory region sequence-specific DNA binding Source: GO_Central
- DNA binding Source: EcoliWiki
- DNA-binding transcription factor activity Source: EcoCyc
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: EcoCyc
- positive regulation of transcription, DNA-templated Source: EcoCyc
- regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:G7331-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Hca operon transcriptional activator HcaR |
Gene namesi | Name:hcaR Synonyms:phdR, yfhT Ordered Locus Names:b2537, JW2521 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105631 | 1 – 296 | Hca operon transcriptional activator HcaRAdd BLAST | 296 |
Proteomic databases
PaxDbi | Q47141 |
PRIDEi | Q47141 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261574, 17 interactors |
IntActi | Q47141, 2 interactors |
STRINGi | 511145.b2537 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 58 | HTH lysR-typePROSITE-ProRule annotationAdd BLAST | 58 |
Sequence similaritiesi
Belongs to the LysR transcriptional regulatory family.Curated
Phylogenomic databases
eggNOGi | COG0583, Bacteria |
HOGENOMi | CLU_039613_6_4_6 |
InParanoidi | Q47141 |
PhylomeDBi | Q47141 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005119, LysR_subst-bd IPR000847, Tscrpt_reg_HTH_LysR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00126, HTH_1, 1 hit PF03466, LysR_substrate, 1 hit |
PRINTSi | PR00039, HTHLYSR |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50931, HTH_LYSR, 1 hit |
i Sequence
Sequence statusi: Complete.
Q47141-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELRHLRYFV AVAQALNFTR AAEKLHTSQP SLSSQIRDLE NCVGVPLLVR
60 70 80 90 100
DKRKVALTAA GECFLQDALA ILEQAENAKL RARKIVQEDR QLTIGFVPSA
110 120 130 140 150
EVNLLPKVLP MFRLRQPDTL IELVSLITTQ QEEKIRRGEL DVGLMRHPVY
160 170 180 190 200
SPEIDYLELF DEPLVVVLPV DHPLAHEKEI TAAQLDGVNF VSTDPVYSGS
210 220 230 240 250
LAPIVKAWFA QENSQPNIVQ VATNILVTMN LVGMGLGVTL IPGYMNNFNT
260 270 280 290
GQVVFRPIAG NVPSIALLMA WKKGEMKPAL RDFIAIVQER LASVTA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 127 – 141 | ITTQQ…RGELD → SPRNRRKKFAVVNST in CAA86020 (Ref. 1) CuratedAdd BLAST | 15 | |
Sequence conflicti | 145 – 148 | MRHP → NCAIH in CAA86020 (Ref. 1) Curated | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z37966 Genomic DNA Translation: CAA86020.1 U00096 Genomic DNA Translation: AAC75590.1 AP009048 Genomic DNA Translation: BAA16432.2 |
PIRi | H65030 |
RefSeqi | NP_417032.1, NC_000913.3 WP_000423261.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75590; AAC75590; b2537 BAA16432; BAA16432; BAA16432 |
GeneIDi | 947000 |
KEGGi | ecj:JW2521 eco:b2537 |
PATRICi | fig|1411691.4.peg.4197 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z37966 Genomic DNA Translation: CAA86020.1 U00096 Genomic DNA Translation: AAC75590.1 AP009048 Genomic DNA Translation: BAA16432.2 |
PIRi | H65030 |
RefSeqi | NP_417032.1, NC_000913.3 WP_000423261.1, NZ_STEB01000011.1 |
3D structure databases
SMRi | Q47141 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261574, 17 interactors |
IntActi | Q47141, 2 interactors |
STRINGi | 511145.b2537 |
Proteomic databases
PaxDbi | Q47141 |
PRIDEi | Q47141 |
Genome annotation databases
EnsemblBacteriai | AAC75590; AAC75590; b2537 BAA16432; BAA16432; BAA16432 |
GeneIDi | 947000 |
KEGGi | ecj:JW2521 eco:b2537 |
PATRICi | fig|1411691.4.peg.4197 |
Organism-specific databases
EchoBASEi | EB3228 |
Phylogenomic databases
eggNOGi | COG0583, Bacteria |
HOGENOMi | CLU_039613_6_4_6 |
InParanoidi | Q47141 |
PhylomeDBi | Q47141 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7331-MONOMER |
Miscellaneous databases
PROi | PR:Q47141 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005119, LysR_subst-bd IPR000847, Tscrpt_reg_HTH_LysR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00126, HTH_1, 1 hit PF03466, LysR_substrate, 1 hit |
PRINTSi | PR00039, HTHLYSR |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50931, HTH_LYSR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HCAR_ECOLI | |
Accessioni | Q47141Primary (citable) accession number: Q47141 Secondary accession number(s): P76583, P77167 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families