hcaR

Functionⁱ

Transcriptional activator of the hca operon for 3-phenylpropionic acid catabolism.

Caution

The 21 C-terminal amino acids have been proposed to be expressed from an independent promoter within this gene and called IroK. Overexpression of IroK has been suggested to be responsible for 3-hydroxypropionic acid (3-HP) resistance. However the IroK peptide's existence has not been proven, nor has mutagenesis of HcaR itself rather than overexpression of IroK been ruled out as the cause of 3-HP resistance (PubMed:22161628).1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	18 – 38	H-T-H motifPROSITE-ProRule annotationAdd BLAST		21

GO - Molecular functionⁱ

DNA binding
Source: EcoliWiki
Inferred from direct assayⁱ
- 15808934
DNA binding transcription factor activity
Source: EcoCyc
Inferred from direct assayⁱ
- 11200225

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of transcription, DNA-templated
Source: EcoCyc
Inferred from direct assayⁱ
- 11200225
positive regulation of transcription, DNA-templated
Source: EcoCyc
Inferred from direct assayⁱ
- 11200225
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

BioCycⁱ	EcoCyc:G7331-MONOMER

BioCycⁱ

EcoCyc:G7331-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Hca operon transcriptional activator HcaR
Gene namesⁱ	Name:hcaR Synonyms:phdR, yfhT Ordered Locus Names:b2537, JW2521
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG13455 hcaR

EcoGeneⁱ

EG13455 hcaR

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000105631	1 – 296	Hca operon transcriptional activator HcaRAdd BLAST		296

Proteomic databases

PaxDbⁱ	Q47141
PRIDEⁱ	Q47141

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4261574, 17 interactors
IntActⁱ	Q47141, 2 interactors
STRINGⁱ	316385.ECDH10B_2704

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q47141
SMRⁱ	Q47141
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	1 – 58	HTH lysR-typePROSITE-ProRule annotationAdd BLAST		58

Phylogenomic databases

eggNOGⁱ	ENOG4108W5M Bacteria ENOG4111IYC LUCA
HOGENOMⁱ	HOG000233514
InParanoidⁱ	Q47141
KEGG Orthology (KO) More...KOⁱ	K05817
OMAⁱ	KGIQQNM
PhylomeDBⁱ	Q47141

Family and domain databases

Gene3Dⁱ	1.10.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR005119 LysR_subst-bd IPR000847 Tscrpt_reg_HTH_LysR IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00126 HTH_1, 1 hit PF03466 LysR_substrate, 1 hit
PRINTSⁱ	PR00039 HTHLYSR
SUPFAMⁱ	SSF46785 SSF46785, 1 hit
PROSITEⁱ	View protein in PROSITE PS50931 HTH_LYSR, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q47141-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MELRHLRYFV AVAQALNFTR AAEKLHTSQP SLSSQIRDLE NCVGVPLLVR 
        60         70         80         90        100
DKRKVALTAA GECFLQDALA ILEQAENAKL RARKIVQEDR QLTIGFVPSA 
       110        120        130        140        150
EVNLLPKVLP MFRLRQPDTL IELVSLITTQ QEEKIRRGEL DVGLMRHPVY 
       160        170        180        190        200
SPEIDYLELF DEPLVVVLPV DHPLAHEKEI TAAQLDGVNF VSTDPVYSGS 
       210        220        230        240        250
LAPIVKAWFA QENSQPNIVQ VATNILVTMN LVGMGLGVTL IPGYMNNFNT 
       260        270        280        290 
GQVVFRPIAG NVPSIALLMA WKKGEMKPAL RDFIAIVQER LASVTA

Length:296

Mass (Da):32,838

Last modified:November 1, 1997 - v2

Checksum:ⁱ72FF08A62D490AAA

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	127 – 141	ITTQQ…RGELD → SPRNRRKKFAVVNST in CAA86020 (Ref. 1) CuratedAdd BLAST		15
Sequence conflictⁱ	145 – 148	MRHP → NCAIH in CAA86020 (Ref. 1) Curated		4

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z37966 Genomic DNA Translation: CAA86020.1 U00096 Genomic DNA Translation: AAC75590.1 AP009048 Genomic DNA Translation: BAA16432.2
PIRⁱ	H65030
NCBI Reference Sequences More...RefSeqⁱ	NP_417032.1, NC_000913.3 WP_000423261.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC75590; AAC75590; b2537 BAA16432; BAA16432; BAA16432
GeneIDⁱ	947000
KEGGⁱ	ecj:JW2521 eco:b2537
PATRICⁱ	fig\|1411691.4.peg.4197

Protein	Similar proteins		Species	Score	Length
Q47141	Hca operon transcriptional activator		ECOLX		296
Hca operon transcriptional activator HcaR		SHIDY		296
Transcriptional regulator		Escherichia coli O111:NM str. K6722		296
LysR family transcriptional regulator		Escherichia coli ATCC BAA-2209		296
Hca operon transcriptional activator protein		Escherichia coli UMNK88		296
+25

Protein	Similar proteins		Species	Score	Length
Q47141	Hca operon transcriptional activator		ECOLX		296
Hca operon transcriptional activator HcaR		SHIDY		296
Transcriptional regulator		Escherichia coli O111:NM str. K6722		296
LysR family transcriptional regulator		Escherichia coli ATCC BAA-2209		296
Hca operon transcriptional activator protein		Escherichia coli UMNK88		296
+343

Protein	Similar proteins		Species	Score	Length
Q47141	Hca operon transcriptional activator		ECOLX		296
Hca operon transcriptional activator HcaR		SHIDY		296
Transcriptional regulator		Escherichia coli O111:NM str. K6722		296
LysR family transcriptional regulator		Escherichia coli ATCC BAA-2209		296
Hca operon transcriptional activator protein		Escherichia coli UMNK88		296
+435

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z37966 Genomic DNA Translation: CAA86020.1 U00096 Genomic DNA Translation: AAC75590.1 AP009048 Genomic DNA Translation: BAA16432.2
PIRⁱ	H65030
NCBI Reference Sequences More...RefSeqⁱ	NP_417032.1, NC_000913.3 WP_000423261.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	Q47141
SMRⁱ	Q47141
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261574, 17 interactors
IntActⁱ	Q47141, 2 interactors
STRINGⁱ	316385.ECDH10B_2704

Proteomic databases

PaxDbⁱ	Q47141
PRIDEⁱ	Q47141

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC75590; AAC75590; b2537 BAA16432; BAA16432; BAA16432
GeneIDⁱ	947000
KEGGⁱ	ecj:JW2521 eco:b2537
PATRICⁱ	fig\|1411691.4.peg.4197

Organism-specific databases

EchoBASEⁱ	EB3228
EcoGeneⁱ	EG13455 hcaR

Phylogenomic databases

eggNOGⁱ	ENOG4108W5M Bacteria ENOG4111IYC LUCA
HOGENOMⁱ	HOG000233514
InParanoidⁱ	Q47141
KEGG Orthology (KO) More...KOⁱ	K05817
OMAⁱ	KGIQQNM
PhylomeDBⁱ	Q47141

Enzyme and pathway databases

BioCycⁱ	EcoCyc:G7331-MONOMER

BioCycⁱ

EcoCyc:G7331-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q47141

PROⁱ

PR:Q47141

Family and domain databases

Gene3Dⁱ	1.10.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR005119 LysR_subst-bd IPR000847 Tscrpt_reg_HTH_LysR IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00126 HTH_1, 1 hit PF03466 LysR_substrate, 1 hit
PRINTSⁱ	PR00039 HTHLYSR
SUPFAMⁱ	SSF46785 SSF46785, 1 hit
PROSITEⁱ	View protein in PROSITE PS50931 HTH_LYSR, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HCAR_ECOLI
Accessionⁱ	Q47141Primary (citable) accession number: Q47141 Secondary accession number(s): P76583, P77167
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	November 1, 1997
	Last modified:	March 28, 2018
	This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

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Annotation systems

Supporting data

UniProtKB - Q47141 (HCAR_ECOLI)

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Hca operon transcriptional activator HcaR

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caution

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ