Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lipoyl synthase

Gene

lipA

Organism
Prochlorococcus marinus (strain NATL2A)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA), Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi61Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi76Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi80Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi83Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

BioCyciPMAR59920:G1G4T-1296-MONOMER
UniPathwayi
UPA00538;UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:PMN2A_0652Imported
OrganismiProchlorococcus marinus (strain NATL2A)Imported
Taxonomic identifieri59920 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000002535 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliQ46K35
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 280Elp3InterPro annotationAdd BLAST215

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000235997
KOiK03644
OMAiGRCPNRG
OrthoDBiPOG091H069D
PhylomeDBiQ46K35

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PANTHERiPTHR10949 PTHR10949, 1 hit
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Q46K35-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTTTTRKTTK YSSFLPEERL PNWIKPSIGN ATQLEKVQKL VKENRLHTIC
60 70 80 90 100
EEGRCPNRGE CYAAGTATFL LGGSICTRSC AFCQVEKGKS PEKVNIFEAE
110 120 130 140 150
KVANAVQVLN LKYVVLTAVA RDDLPDHGAS LFTTTMDAIR SLNPGVAIEV
160 170 180 190 200
LTPDFWGGNN KDNVEKQRER LKLVLSANPI CFNHNLETVK RLQREVRRGA
210 220 230 240 250
TYQHSLNLLK FAREIDPKIP TKSGIMLGLG EKFHEIIQTL KDLRKVDCQY
260 270 280 290 300
LTIGQYLRPS LAHIPVSHYW SPTKFNDFAE IANGLGFKKV NSGPLVRSSY

HAKETLD
Length:307
Mass (Da):34,475
Last modified:September 13, 2005 - v1
Checksum:i12C45C397732611F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000095 Genomic DNA Translation: AAZ58143.1
RefSeqiWP_011294741.1, NC_007335.2

Genome annotation databases

EnsemblBacteriaiAAZ58143; AAZ58143; PMN2A_0652
KEGGipmn:PMN2A_0652

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000095 Genomic DNA Translation: AAZ58143.1
RefSeqiWP_011294741.1, NC_007335.2

3D structure databases

ProteinModelPortaliQ46K35
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ58143; AAZ58143; PMN2A_0652
KEGGipmn:PMN2A_0652

Phylogenomic databases

HOGENOMiHOG000235997
KOiK03644
OMAiGRCPNRG
OrthoDBiPOG091H069D
PhylomeDBiQ46K35

Enzyme and pathway databases

UniPathwayi
UPA00538;UER00593

BioCyciPMAR59920:G1G4T-1296-MONOMER

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PANTHERiPTHR10949 PTHR10949, 1 hit
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiQ46K35_PROMT
AccessioniPrimary (citable) accession number: Q46K35
Entry historyiIntegrated into UniProtKB/TrEMBL: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 7, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again