UniProtKB - Q46927 (TCDA_ECOLI)
Protein
tRNA threonylcarbamoyladenosine dehydratase
Gene
tcdA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the ATP-dependent dehydration of threonylcarbamoyladenosine at position 37 (t6A37) to form cyclic t6A37 (ct6A37) in tRNAs that read codons beginning with adenine. TcdA is also part of a sulfur transfer pathway; is able to accept sulfur from CsdA directly in vitro, but CsdE might act as the sulfur donor in vivo.2 Publications
Miscellaneous
ct6A is involved in promoting decoding efficiency. It is an unstable modification that can be easily hydrolyzed and converted to t6A during nucleoside preparation by conventional methods (PubMed:23242255). This explains why it was described as t6A37 in earlier studies.1 Publication
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- potassium ion binding Source: EcoCyc
- sodium ion binding Source: EcoCyc
- tRNA threonylcarbamoyladenosine dehydratase Source: EcoCyc
- ubiquitin-like modifier activating enzyme activity Source: InterPro
GO - Biological processi
- cyclic threonylcarbamoyladenosine biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G7456-MONOMER MetaCyc:G7456-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA threonylcarbamoyladenosine dehydratase (EC:6.1.-.-)Alternative name(s): t(6)A37 dehydratase |
Gene namesi | Name:tcdA Synonyms:csdL, ygdL Ordered Locus Names:b2812, JW2783 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Membrane Curated; Single-pass membrane protein Curated
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 237 – 257 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
Cells lacking this gene have a normal growth phenotype, but are unable to survive in a competitive growth situation with the wild-type strain. They display only the t6A but not the ct6A modification in tRNAs, and have lower decoding efficiency than wild-type. They show no defects in motility or antibiotic sensitivity. In growth competition experiments, a tcdA mutant shows reduced fitness compared to wild-type, but outcompetes a csdA mutant.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120579 | 1 – 268 | tRNA threonylcarbamoyladenosine dehydrataseAdd BLAST | 268 |
Proteomic databases
PaxDbi | Q46927 |
PRIDEi | Q46927 |
Interactioni
Subunit structurei
Interacts with CsdE.
1 PublicationBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4261127, 834 interactors |
IntActi | Q46927, 9 interactors |
STRINGi | 511145.b2812 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q46927 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the HesA/MoeB/ThiF family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1179, Bacteria |
HOGENOMi | CLU_013325_4_0_6 |
InParanoidi | Q46927 |
PhylomeDBi | Q46927 |
Family and domain databases
InterProi | View protein in InterPro IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz |
Pfami | View protein in Pfam PF00899, ThiF, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
i Sequence
Sequence statusi: Complete.
Q46927-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVVISDAWR QRFGGTARLY GEKALQLFAD AHICVVGIGG VGSWAAEALA
60 70 80 90 100
RTGIGAITLI DMDDVCVTNT NRQIHALRDN VGLAKAEVMA ERIRQINPEC
110 120 130 140 150
RVTVVDDFVT PDNVAQYMSV GYSYVIDAID SVRPKAALIA YCRRNKIPLV
160 170 180 190 200
TTGGAGGQID PTQIQVTDLA KTIQDPLAAK LRERLKSDFG VVKNSKGKLG
210 220 230 240 250
VDCVFSTEAL VYPQSDGTVC AMKATAEGPK RMDCASGFGA ATMVTATFGF
260
VAVSHALKKM MAKAARQG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U29581 Genomic DNA Translation: AAB40462.1 U00096 Genomic DNA Translation: AAC75854.1 AP009048 Genomic DNA Translation: BAE76884.1 |
PIRi | H65063 |
RefSeqi | NP_417292.1, NC_000913.3 WP_000117728.1, NZ_SSZK01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC75854; AAC75854; b2812 BAE76884; BAE76884; BAE76884 |
GeneIDi | 57730384 947291 |
KEGGi | ecj:JW2783 eco:b2812 |
PATRICi | fig|511145.12.peg.2912 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U29581 Genomic DNA Translation: AAB40462.1 U00096 Genomic DNA Translation: AAC75854.1 AP009048 Genomic DNA Translation: BAE76884.1 |
PIRi | H65063 |
RefSeqi | NP_417292.1, NC_000913.3 WP_000117728.1, NZ_SSZK01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4D79 | X-ray | 1.77 | A/B/C/D | 1-268 | [»] | |
4D7A | X-ray | 1.80 | A/B/C/D | 1-268 | [»] | |
4RDH | X-ray | 2.10 | A/B/C/D | 1-268 | [»] | |
4RDI | X-ray | 1.95 | A/B/C/D | 1-268 | [»] | |
4YED | X-ray | 1.90 | A/B/C/D | 1-268 | [»] | |
SMRi | Q46927 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261127, 834 interactors |
IntActi | Q46927, 9 interactors |
STRINGi | 511145.b2812 |
Proteomic databases
PaxDbi | Q46927 |
PRIDEi | Q46927 |
Genome annotation databases
EnsemblBacteriai | AAC75854; AAC75854; b2812 BAE76884; BAE76884; BAE76884 |
GeneIDi | 57730384 947291 |
KEGGi | ecj:JW2783 eco:b2812 |
PATRICi | fig|511145.12.peg.2912 |
Organism-specific databases
EchoBASEi | EB2893 |
Phylogenomic databases
eggNOGi | COG1179, Bacteria |
HOGENOMi | CLU_013325_4_0_6 |
InParanoidi | Q46927 |
PhylomeDBi | Q46927 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7456-MONOMER MetaCyc:G7456-MONOMER |
Miscellaneous databases
PROi | PR:Q46927 |
Family and domain databases
InterProi | View protein in InterPro IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz |
Pfami | View protein in Pfam PF00899, ThiF, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TCDA_ECOLI | |
Accessioni | Q46927Primary (citable) accession number: Q46927 Secondary accession number(s): Q2MA22 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families