UniProtKB - Q46899 (CASC_ECOLI)
CRISPR system Cascade subunit CasC
casC
Functioni
CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).
A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasCDE alone is also able to form R-loops.
GO - Molecular functioni
- DNA/RNA hybrid binding Source: EcoCyc
- RNA binding Source: EcoCyc
GO - Biological processi
- CRISPR-cas system Source: ComplexPortal
- defense response to virus Source: EcoCyc
Keywordsi
Molecular function | RNA-binding |
Biological process | Antiviral defense |
Enzyme and pathway databases
BioCyci | EcoCyc:G7428-MONOMER |
BRENDAi | 3.1.12.1, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: CRISPR system Cascade subunit CasC |
Gene namesi | Name:casC Synonyms:cas4, cse4, ygcJ Ordered Locus Names:b2758, JW2728 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCurated | |||
ChainiPRO_0000169322 | 2 – 363 | CRISPR system Cascade subunit CasCAdd BLAST | 362 |
Proteomic databases
PaxDbi | Q46899 |
PRIDEi | Q46899 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Part of the Cascade ribonucleoprotein complex, with stoichiometry CasA1,CasB2,CasC6,CasD1,CasE1-crRNA1. The 6 CasC subunits make a helical stack forming a groove in which the crRNA lies.
Interacts directly with all of the Cas subunits. Stable subcomplexes of CasBCDE-crRNA and CasCDE-crRNA also form, both of which are able to bind target dsDNA, and at least CasCDE is able to form R-loops. CasCDE and CasCE complexes have endonuclease activity.
Interacts with YgbT (Cas1). Binding of target ssRNA or dsDNA causes a conformational change in the Cascade complex.
7 PublicationsProtein-protein interaction databases
BioGRIDi | 4262275, 608 interactors |
ComplexPortali | CPX-1005, Cascade complex |
DIPi | DIP-12126N |
IntActi | Q46899, 4 interactors |
STRINGi | 511145.b2758 |
Structurei
Secondary structure
3D structure databases
SMRi | Q46899 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Phylogenomic databases
eggNOGi | COG1857, Bacteria |
HOGENOMi | CLU_044824_1_0_6 |
InParanoidi | Q46899 |
PhylomeDBi | Q46899 |
Family and domain databases
CDDi | cd09646, Cas7_I-E, 1 hit |
InterProi | View protein in InterPro IPR010148, CRISPR-assoc_prot_CT1975 |
Pfami | View protein in Pfam PF09344, Cas_CT1975, 1 hit |
TIGRFAMsi | TIGR01869, casC_Cse4, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSNFINIHVL ISHSPSCLNR DDMNMQKDAI FGGKRRVRIS SQSLKRAMRK
60 70 80 90 100
SGYYAQNIGE SSLRTIHLAQ LRDVLRQKLG ERFDQKIIDK TLALLSGKSV
110 120 130 140 150
DEAEKISADA VTPWVVGEIA WFCEQVAKAE ADNLDDKKLL KVLKEDIAAI
160 170 180 190 200
RVNLQQGVDI ALSGRMATSG MMTELGKVDG AMSIAHAITT HQVDSDIDWF
210 220 230 240 250
TAVDDLQEQG SAHLGTQEFS SGVFYRYANI NLAQLQENLG GASREQALEI
260 270 280 290 300
ATHVVHMLAT EVPGAKQRTY AAFNPADMVM VNFSDMPLSM ANAFEKAVKA
310 320 330 340 350
KDGFLQPSIQ AFNQYWDRVA NGYGLNGAAA QFSLSDVDPI TAQVKQMPTL
360
EQLKSWVRNN GEA
Mass spectrometryi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U29579 Genomic DNA Translation: AAA69268.1 U00096 Genomic DNA Translation: AAC75800.1 AP009048 Genomic DNA Translation: BAE76835.1 |
PIRi | B65057 |
RefSeqi | NP_417238.1, NC_000913.3 WP_000064450.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75800; AAC75800; b2758 BAE76835; BAE76835; BAE76835 |
GeneIDi | 947224 |
KEGGi | ecj:JW2728 eco:b2758 |
PATRICi | fig|1411691.4.peg.3980 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U29579 Genomic DNA Translation: AAA69268.1 U00096 Genomic DNA Translation: AAC75800.1 AP009048 Genomic DNA Translation: BAE76835.1 |
PIRi | B65057 |
RefSeqi | NP_417238.1, NC_000913.3 WP_000064450.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4QYZ | X-ray | 3.03 | D/E/F/G/H/I | 1-363 | [»] | |
4TVX | X-ray | 3.24 | B/C/D/E/F/G/N/O/P/Q/R/S | 1-363 | [»] | |
4U7U | X-ray | 3.00 | E/F/G/H/I/J/Q/R/S/T/U/V | 1-363 | [»] | |
5CD4 | X-ray | 3.20 | B/C/D/E/F/G/N/O/P/Q/R/S | 1-363 | [»] | |
5H9E | X-ray | 3.21 | D/E/F/G/H/I | 1-363 | [»] | |
5H9F | X-ray | 2.45 | D/E/F/G/H/I | 1-363 | [»] | |
SMRi | Q46899 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262275, 608 interactors |
ComplexPortali | CPX-1005, Cascade complex |
DIPi | DIP-12126N |
IntActi | Q46899, 4 interactors |
STRINGi | 511145.b2758 |
Proteomic databases
PaxDbi | Q46899 |
PRIDEi | Q46899 |
Genome annotation databases
EnsemblBacteriai | AAC75800; AAC75800; b2758 BAE76835; BAE76835; BAE76835 |
GeneIDi | 947224 |
KEGGi | ecj:JW2728 eco:b2758 |
PATRICi | fig|1411691.4.peg.3980 |
Organism-specific databases
EchoBASEi | EB2918 |
Phylogenomic databases
eggNOGi | COG1857, Bacteria |
HOGENOMi | CLU_044824_1_0_6 |
InParanoidi | Q46899 |
PhylomeDBi | Q46899 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7428-MONOMER |
BRENDAi | 3.1.12.1, 2026 |
Miscellaneous databases
PROi | PR:Q46899 |
Family and domain databases
CDDi | cd09646, Cas7_I-E, 1 hit |
InterProi | View protein in InterPro IPR010148, CRISPR-assoc_prot_CT1975 |
Pfami | View protein in Pfam PF09344, Cas_CT1975, 1 hit |
TIGRFAMsi | TIGR01869, casC_Cse4, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CASC_ECOLI | |
Accessioni | Q46899Primary (citable) accession number: Q46899 Secondary accession number(s): Q2MA71 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references