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UniProtKB - Q46893 (ISPD_ECOLI)
Protein
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Gene
ispD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).
Miscellaneous
There are no coordination bonds that occur between IspD and magnesium in any of the complexes examined to date.
Catalytic activityi
- EC:2.7.7.60
Cofactori
pH dependencei
Optimum pH is 8.3.
: isopentenyl diphosphate biosynthesis via DXP pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate. This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 20 | Transition state stabilizer1 Publication | 1 | |
Sitei | 27 | Transition state stabilizer1 Publication | 1 | |
Sitei | 157 | Positions MEP for the nucleophilic attack1 Publication | 1 | |
Sitei | 213 | Positions MEP for the nucleophilic attack1 Publication | 1 |
GO - Molecular functioni
- 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
- magnesium ion binding Source: EcoCyc
GO - Biological processi
- isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
- terpenoid biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Isoprene biosynthesis |
Ligand | Cobalt, Magnesium, Manganese |
Enzyme and pathway databases
BioCyci | EcoCyc:G7423-MONOMER |
BRENDAi | 2.7.7.60, 2026 |
SABIO-RKi | Q46893 |
UniPathwayi | UPA00056;UER00093 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC:2.7.7.60)Alternative name(s): 4-diphosphocytidyl-2C-methyl-D-erythritol synthase CDP-ME synthase MEP cytidylyltransferase Short name: MCT |
Gene namesi | Name:ispD Synonyms:ygbP Ordered Locus Names:b2747, JW2717 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 27 | K → A or S: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 140 | T → I: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 191 | E → K: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 213 | K → S: Decrease in activity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB03687, 4-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol DB03854, Cadaverine DB02431, Cytidine-5'-Triphosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000075572 | 2 – 236 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseAdd BLAST | 235 |
Proteomic databases
jPOSTi | Q46893 |
PaxDbi | Q46893 |
PRIDEi | Q46893 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4262280, 173 interactors |
IntActi | Q46893, 2 interactors |
STRINGi | 511145.b2747 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q46893 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q46893 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1211, Bacteria |
HOGENOMi | CLU_061281_3_1_6 |
InParanoidi | Q46893 |
PhylomeDBi | Q46893 |
Family and domain databases
CDDi | cd02516, CDP-ME_synthetase, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
HAMAPi | MF_00108, IspD, 1 hit |
InterProi | View protein in InterPro IPR001228, IspD IPR034683, IspD/TarI IPR018294, ISPD_synthase_CS IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF01128, IspD, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR00453, ispD, 1 hit |
PROSITEi | View protein in PROSITE PS01295, ISPD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q46893-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATTHLDVCA VVPAAGFGRR MQTECPKQYL SIGNQTILEH SVHALLAHPR
60 70 80 90 100
VKRVVIAISP GDSRFAQLPL ANHPQITVVD GGDERADSVL AGLKAAGDAQ
110 120 130 140 150
WVLVHDAARP CLHQDDLARL LALSETSRTG GILAAPVRDT MKRAEPGKNA
160 170 180 190 200
IAHTVDRNGL WHALTPQFFP RELLHDCLTR ALNEGATITD EASALEYCGF
210 220 230
HPQLVEGRAD NIKVTRPEDL ALAEFYLTRT IHQENT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF230736 Genomic DNA Translation: AAF43207.1 AB037143 Genomic DNA Translation: BAA90761.1 U29579 Genomic DNA Translation: AAA69257.1 U00096 Genomic DNA Translation: AAC75789.1 AP009048 Genomic DNA Translation: BAE76824.1 |
PIRi | G65055 |
RefSeqi | NP_417227.1, NC_000913.3 WP_000246138.1, NZ_STEB01000027.1 |
Genome annotation databases
EnsemblBacteriai | AAC75789; AAC75789; b2747 BAE76824; BAE76824; BAE76824 |
GeneIDi | 948269 |
KEGGi | ecj:JW2717 eco:b2747 |
PATRICi | fig|1411691.4.peg.3993 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF230736 Genomic DNA Translation: AAF43207.1 AB037143 Genomic DNA Translation: BAA90761.1 U29579 Genomic DNA Translation: AAA69257.1 U00096 Genomic DNA Translation: AAC75789.1 AP009048 Genomic DNA Translation: BAE76824.1 |
PIRi | G65055 |
RefSeqi | NP_417227.1, NC_000913.3 WP_000246138.1, NZ_STEB01000027.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1H3M | X-ray | 2.40 | A/B | 2-236 | [»] | |
1I52 | X-ray | 1.50 | A | 1-236 | [»] | |
1INI | X-ray | 1.82 | A | 1-236 | [»] | |
1INJ | X-ray | 1.55 | A | 1-236 | [»] | |
1VGT | X-ray | 1.80 | A/B | 2-236 | [»] | |
1VGU | X-ray | 2.80 | A/B | 2-236 | [»] | |
3N9W | X-ray | 1.90 | A/B | 2-236 | [»] | |
SMRi | Q46893 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262280, 173 interactors |
IntActi | Q46893, 2 interactors |
STRINGi | 511145.b2747 |
Chemistry databases
DrugBanki | DB03687, 4-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol DB03854, Cadaverine DB02431, Cytidine-5'-Triphosphate |
Proteomic databases
jPOSTi | Q46893 |
PaxDbi | Q46893 |
PRIDEi | Q46893 |
Genome annotation databases
EnsemblBacteriai | AAC75789; AAC75789; b2747 BAE76824; BAE76824; BAE76824 |
GeneIDi | 948269 |
KEGGi | ecj:JW2717 eco:b2747 |
PATRICi | fig|1411691.4.peg.3993 |
Organism-specific databases
EchoBASEi | EB2913 |
Phylogenomic databases
eggNOGi | COG1211, Bacteria |
HOGENOMi | CLU_061281_3_1_6 |
InParanoidi | Q46893 |
PhylomeDBi | Q46893 |
Enzyme and pathway databases
UniPathwayi | UPA00056;UER00093 |
BioCyci | EcoCyc:G7423-MONOMER |
BRENDAi | 2.7.7.60, 2026 |
SABIO-RKi | Q46893 |
Miscellaneous databases
EvolutionaryTracei | Q46893 |
PROi | PR:Q46893 |
Family and domain databases
CDDi | cd02516, CDP-ME_synthetase, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
HAMAPi | MF_00108, IspD, 1 hit |
InterProi | View protein in InterPro IPR001228, IspD IPR034683, IspD/TarI IPR018294, ISPD_synthase_CS IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF01128, IspD, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR00453, ispD, 1 hit |
PROSITEi | View protein in PROSITE PS01295, ISPD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ISPD_ECOLI | |
Accessioni | Q46893Primary (citable) accession number: Q46893 Secondary accession number(s): Q2MA82 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 176 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families