UniProtKB - Q46871 (YQJH_ECOLI)
Protein
NADPH-dependent ferric-chelate reductase
Gene
yqjH
Organism
Escherichia coli (strain K12)
Status
Functioni
Plays a role in iron homeostasis under excess nickel conditions.1 Publication
Catalytic activityi
- EC:1.16.1.91 Publication
Kineticsi
- KM=33 µM for ferric ions (anaerobically at pH 7.5)1 Publication
- KM=43 µM for NADPH (anaerobically at pH 7.5)1 Publication
GO - Molecular functioni
- FAD binding Source: EcoCyc
- ferric-chelate reductase (NADPH) activity Source: UniProtKB
- flavin adenine dinucleotide binding Source: EcoCyc
GO - Biological processi
- cellular response to iron ion starvation Source: GO_Central
- cellular response to nickel ion Source: EcoCyc
- iron import into cell Source: GO_Central
- siderophore-dependent iron import into cell Source: EcoCyc
- siderophore transport Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:G7593-MONOMER MetaCyc:G7593-MONOMER |
SABIO-RKi | Q46871 |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH-dependent ferric-chelate reductase (EC:1.16.1.9)Alternative name(s): Ferric siderophore reductase |
Gene namesi | Name:yqjH Ordered Locus Names:b3070, JW3041 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Inactivation leads to ferrous iron chelator resistance as the wild-type.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169437 | 1 – 254 | NADPH-dependent ferric-chelate reductaseAdd BLAST | 254 |
Proteomic databases
PaxDbi | Q46871 |
PRIDEi | Q46871 |
Expressioni
Inductioni
Repressed by YqjI and Fur. YqjI is required for nickel-dependent regulation of yqjH, while Fur is required for iron- and cobalt-dependent regulation of yqjH.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259593, 17 interactors 851898, 1 interactor |
IntActi | Q46871, 3 interactors |
STRINGi | 511145.b3070 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 15 – 136 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 122 |
Sequence similaritiesi
Belongs to the SIP oxidoreductase family.Curated
Phylogenomic databases
eggNOGi | COG2375, Bacteria |
HOGENOMi | CLU_040923_4_0_6 |
InParanoidi | Q46871 |
PhylomeDBi | Q46871 |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR013113, FAD-bd_9_SIP IPR017927, FAD-bd_FR_type IPR039261, FNR_nucleotide-bd IPR017938, Riboflavin_synthase-like_b-brl IPR007037, SIP_C IPR039374, SIP_fam |
PANTHERi | PTHR30157, PTHR30157, 1 hit |
Pfami | View protein in Pfam PF08021, FAD_binding_9, 1 hit PF04954, SIP, 1 hit |
SUPFAMi | SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
i Sequence
Sequence statusi: Complete.
Q46871-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNNTPRYPQR VRNDLRFREL TVLRVERISA GFQRIVLGGE ALDGFTSRGF
60 70 80 90 100
DDHSKLFFPQ PDAHFVPPTV TEEGIVWPEG PRPPSRDYTP LYDELRHELA
110 120 130 140 150
IDFFIHDGGV ASGWAMQAQP GDKLTVAGPR GSLVVPEDYA YQLYVCDESG
160 170 180 190 200
MPALRRRLET LSKLAVKPQV SALVSVRDNA CQDYLAHLDG FNIEWLAHDE
210 220 230 240 250
QAVDARLAQM QIPADDYFIW ITGEGKVVKN LSRRFEAEQY DPQRVRAAAY
WHAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28379 Genomic DNA Translation: AAA89149.1 U00096 Genomic DNA Translation: AAC76105.1 AP009048 Genomic DNA Translation: BAE77120.1 |
PIRi | C65095 |
RefSeqi | NP_417541.1, NC_000913.3 WP_001066494.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76105; AAC76105; b3070 BAE77120; BAE77120; BAE77120 |
GeneIDi | 57728197 947582 |
KEGGi | ecj:JW3041 eco:b3070 |
PATRICi | fig|511145.12.peg.3164 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28379 Genomic DNA Translation: AAA89149.1 U00096 Genomic DNA Translation: AAC76105.1 AP009048 Genomic DNA Translation: BAE77120.1 |
PIRi | C65095 |
RefSeqi | NP_417541.1, NC_000913.3 WP_001066494.1, NZ_LN832404.1 |
3D structure databases
SMRi | Q46871 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259593, 17 interactors 851898, 1 interactor |
IntActi | Q46871, 3 interactors |
STRINGi | 511145.b3070 |
Proteomic databases
PaxDbi | Q46871 |
PRIDEi | Q46871 |
Genome annotation databases
EnsemblBacteriai | AAC76105; AAC76105; b3070 BAE77120; BAE77120; BAE77120 |
GeneIDi | 57728197 947582 |
KEGGi | ecj:JW3041 eco:b3070 |
PATRICi | fig|511145.12.peg.3164 |
Organism-specific databases
EchoBASEi | EB2787 |
Phylogenomic databases
eggNOGi | COG2375, Bacteria |
HOGENOMi | CLU_040923_4_0_6 |
InParanoidi | Q46871 |
PhylomeDBi | Q46871 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7593-MONOMER MetaCyc:G7593-MONOMER |
SABIO-RKi | Q46871 |
Miscellaneous databases
PROi | PR:Q46871 |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR013113, FAD-bd_9_SIP IPR017927, FAD-bd_FR_type IPR039261, FNR_nucleotide-bd IPR017938, Riboflavin_synthase-like_b-brl IPR007037, SIP_C IPR039374, SIP_fam |
PANTHERi | PTHR30157, PTHR30157, 1 hit |
Pfami | View protein in Pfam PF08021, FAD_binding_9, 1 hit PF04954, SIP, 1 hit |
SUPFAMi | SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YQJH_ECOLI | |
Accessioni | Q46871Primary (citable) accession number: Q46871 Secondary accession number(s): Q2M9D6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families