UniProtKB - Q46868 (UBIK_ECOLI)
Protein
Ubiquinone biosynthesis accessory factor UbiK
Gene
ubiK
Organism
Escherichia coli (strain K12)
Status
Functioni
Required for efficient ubiquinone (coenzyme Q) biosynthesis under aerobic conditions (PubMed:28559279, PubMed:30686758). UbiK is probably an accessory factor of Ubi enzymes and facilitates ubiquinone biosynthesis by acting as an assembly factor, a targeting factor, or both (PubMed:28559279). Dispensable for ubiquinone biosynthesis under anaerobiosis (PubMed:28559279).2 Publications
: ubiquinone biosynthesis Pathwayi
This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.
GO - Molecular functioni
- identical protein binding Source: EcoCyc
GO - Biological processi
- ubiquinone biosynthetic process Source: EcoCyc
Keywordsi
Biological process | Ubiquinone biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:G7582-MONOMER MetaCyc:G7582-MONOMER |
UniPathwayi | UPA00232 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquinone biosynthesis accessory factor UbiKUniRule annotationCurated |
Gene namesi | Name:ubiK1 PublicationUniRule annotation Synonyms:yqiC Ordered Locus Names:b3042, JW5505 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Cytosol
- cytosol Source: EcoCyc
Other locations
- membrane Source: EcoCyc
- ubiquinone biosynthesis complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Deletion of the gene impacts ubiquinone biosynthesis in aerobic conditions but does not decrease menaquinone biosynthesis. Mutant accumulates octaprenylphenol, an early intermediate of the ubiquinone biosynthetic pathway.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169417 | 1 – 96 | Ubiquinone biosynthesis accessory factor UbiKAdd BLAST | 96 |
Proteomic databases
jPOSTi | Q46868 |
PaxDbi | Q46868 |
PRIDEi | Q46868 |
Interactioni
Subunit structurei
Homotrimer (PubMed:28559279).
Component of the Ubi complex metabolon, which regroups five ubiquinone biosynthesis proteins (UbiE, UbiF, UbiG, UbiH and UbiI) and two accessory factors (UbiK and the lipid-binding protein UbiJ) (PubMed:30686758).
Interacts with the C-terminal part of UbiJ and forms a complex composed of 2 UbiK subunits and 1 UbiJ subunit. The UbiK-UbiJ complex interacts with palmitoleic acid (PubMed:28559279).
2 PublicationsGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4259251, 14 interactors 851841, 3 interactors |
IntActi | Q46868, 3 interactors |
STRINGi | 511145.b3042 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 59 – 86 | UniRule annotationAdd BLAST | 28 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | COG2960, Bacteria |
HOGENOMi | CLU_154412_0_2_6 |
InParanoidi | Q46868 |
PhylomeDBi | Q46868 |
Family and domain databases
HAMAPi | MF_02216, UbiK, 1 hit |
InterProi | View protein in InterPro IPR007475, UbiK |
PANTHERi | PTHR38040, PTHR38040, 1 hit |
Pfami | View protein in Pfam PF04380, BMFP, 1 hit |
i Sequence
Sequence statusi: Complete.
Q46868-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIDPKKIEQI ARQVHESMPK GIREFGEDVE KKIRQTLQAQ LTRLDLVSRE
60 70 80 90
EFDVQTQVLL RTREKLALLE QRISELENRS TEIKKQPDPE TLPPTL
Sequence cautioni
The sequence AAA69210 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69210.1 Different initiation. U00096 Genomic DNA Translation: AAC76078.2 AP009048 Genomic DNA Translation: BAE77098.1 |
PIRi | H65091 |
RefSeqi | NP_417514.4, NC_000913.3 WP_001295626.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC76078; AAC76078; b3042 BAE77098; BAE77098; BAE77098 |
GeneIDi | 57729137 947524 |
KEGGi | ecj:JW5505 eco:b3042 |
PATRICi | fig|511145.12.peg.3135 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69210.1 Different initiation. U00096 Genomic DNA Translation: AAC76078.2 AP009048 Genomic DNA Translation: BAE77098.1 |
PIRi | H65091 |
RefSeqi | NP_417514.4, NC_000913.3 WP_001295626.1, NZ_STEB01000001.1 |
3D structure databases
SMRi | Q46868 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259251, 14 interactors 851841, 3 interactors |
IntActi | Q46868, 3 interactors |
STRINGi | 511145.b3042 |
Proteomic databases
jPOSTi | Q46868 |
PaxDbi | Q46868 |
PRIDEi | Q46868 |
Genome annotation databases
EnsemblBacteriai | AAC76078; AAC76078; b3042 BAE77098; BAE77098; BAE77098 |
GeneIDi | 57729137 947524 |
KEGGi | ecj:JW5505 eco:b3042 |
PATRICi | fig|511145.12.peg.3135 |
Organism-specific databases
EchoBASEi | EB2847 |
Phylogenomic databases
eggNOGi | COG2960, Bacteria |
HOGENOMi | CLU_154412_0_2_6 |
InParanoidi | Q46868 |
PhylomeDBi | Q46868 |
Enzyme and pathway databases
UniPathwayi | UPA00232 |
BioCyci | EcoCyc:G7582-MONOMER MetaCyc:G7582-MONOMER |
Miscellaneous databases
PROi | PR:Q46868 |
Family and domain databases
HAMAPi | MF_02216, UbiK, 1 hit |
InterProi | View protein in InterPro IPR007475, UbiK |
PANTHERi | PTHR38040, PTHR38040, 1 hit |
Pfami | View protein in Pfam PF04380, BMFP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBIK_ECOLI | |
Accessioni | Q46868Primary (citable) accession number: Q46868 Secondary accession number(s): Q2M9F8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | December 4, 2007 | |
Last modified: | February 10, 2021 | |
This is version 121 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families