UniProtKB - Q46863 (YGIS_ECOLI)
Protein
Probable deoxycholate-binding periplasmic protein YgiS
Gene
ygiS
Organism
Escherichia coli (strain K12)
Status
Functioni
Probably part of a deoxycholate transport system. Its expression in the presence of deoxycholate in a ygiS deletion mutant increases intracellular deoxycholate levels and decreases cell growth; higher expression in the presence of deoxycholate inhibits cell growth completely. Bile acid detergents such as deoxycholate are important for host defense against bacterial growth in the gall bladder and duodenum.1 Publication
GO - Molecular functioni
- peptide transmembrane transporter activity Source: GO_Central
GO - Biological processi
- bile acid and bile salt transport Source: EcoCyc
- peptide transport Source: GO_Central
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:YGIS-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.5.41, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Probable deoxycholate-binding periplasmic protein YgiS |
Gene namesi | Name:ygiS Ordered Locus Names:b3020, JW2988 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Periplasm 1 Publication
GO - Cellular componenti
- ATP-binding cassette (ABC) transporter complex Source: InterPro
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Disruption phenotypei
Cells grow better in the presence of the bile acid deoxycholate. Increased membrane stability when incubated for 2 hours in the presence of 4.5% deoxycholate. Increased cell survival in the presence of 20% deoxycholate.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000031806 | 21 – 535 | Probable deoxycholate-binding periplasmic protein YgiSAdd BLAST | 515 |
Proteomic databases
jPOSTi | Q46863 |
PaxDbi | Q46863 |
PRIDEi | Q46863 |
Expressioni
Inductioni
Repressed by the toxin-antitoxin system MqsR-MqsA; this mRNA is degraded by mRNA interferase MqsR.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259246, 6 interactors |
STRINGi | 511145.b3020 |
Family & Domainsi
Sequence similaritiesi
Belongs to the bacterial solute-binding protein 5 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG4166, Bacteria |
HOGENOMi | CLU_017028_0_3_6 |
InParanoidi | Q46863 |
PhylomeDBi | Q46863 |
Family and domain databases
InterProi | View protein in InterPro IPR030678, Peptide/Ni-bd IPR039424, SBP_5 IPR000914, SBP_5_dom |
PANTHERi | PTHR30290, PTHR30290, 1 hit |
Pfami | View protein in Pfam PF00496, SBP_bac_5, 1 hit |
PIRSFi | PIRSF002741, MppA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q46863-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYTRNLLWLV SLVSAAPLYA ADVPANTPLA PQQVFRYNNH SDPGTLDPQK
60 70 80 90 100
VEENTAAQIV LDLFEGLVWM DGEGQVQPAQ AERWEILDGG KRYIFHLRSG
110 120 130 140 150
LQWSDGQPLT AEDFVLGWQR AVDPKTASPF AGYLAQAHIN NAAAIVAGKA
160 170 180 190 200
DVTSLGVKAT DDRTLEVTLE QPVPWFTTML AWPTLFPVPH HVIAKHGDSW
210 220 230 240 250
SKPENMVYNG AFVLDQWVVN EKITARKNPK YRDAQHTVLQ QVEYLALDNS
260 270 280 290 300
VTGYNRYRAG EVDLTWVPAQ QIPAIEKSLP GELRIIPRLN SEYYNFNLEK
310 320 330 340 350
PPFNDVRVRR ALYLTVDRQL IAQKVLGLRT PATTLTPPEV KGFSATTFDE
360 370 380 390 400
LQKPMSERVA MAKALLKQAG YDASHPLRFE LFYNKYDLHE KTAIALSSEW
410 420 430 440 450
KKWLGAQVTL RTMEWKTYLD ARRAGDFMLS RQSWDATYND ASSFLNTLKS
460 470 480 490 500
DSEENVGHWK NAQYDALLNQ ATQITDATKR NALYQQAEVI INQQAPLIPI
510 520 530
YYQPLIKLLK PYVGGFPLHN PQDYVYSKEL YIKAH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69188.1 U00096 Genomic DNA Translation: AAC76056.1 AP009048 Genomic DNA Translation: BAE77076.1 |
PIRi | B65089 |
RefSeqi | NP_417492.1, NC_000913.3 WP_001295629.1, NZ_SSZK01000023.1 |
Genome annotation databases
EnsemblBacteriai | AAC76056; AAC76056; b3020 BAE77076; BAE77076; BAE77076 |
GeneIDi | 947140 |
KEGGi | ecj:JW2988 eco:b3020 |
PATRICi | fig|1411691.4.peg.3710 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69188.1 U00096 Genomic DNA Translation: AAC76056.1 AP009048 Genomic DNA Translation: BAE77076.1 |
PIRi | B65089 |
RefSeqi | NP_417492.1, NC_000913.3 WP_001295629.1, NZ_SSZK01000023.1 |
3D structure databases
SMRi | Q46863 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259246, 6 interactors |
STRINGi | 511145.b3020 |
Protein family/group databases
TCDBi | 3.A.1.5.41, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | Q46863 |
PaxDbi | Q46863 |
PRIDEi | Q46863 |
Genome annotation databases
EnsemblBacteriai | AAC76056; AAC76056; b3020 BAE77076; BAE77076; BAE77076 |
GeneIDi | 947140 |
KEGGi | ecj:JW2988 eco:b3020 |
PATRICi | fig|1411691.4.peg.3710 |
Organism-specific databases
EchoBASEi | EB2839 |
Phylogenomic databases
eggNOGi | COG4166, Bacteria |
HOGENOMi | CLU_017028_0_3_6 |
InParanoidi | Q46863 |
PhylomeDBi | Q46863 |
Enzyme and pathway databases
BioCyci | EcoCyc:YGIS-MONOMER |
Miscellaneous databases
PROi | PR:Q46863 |
Family and domain databases
InterProi | View protein in InterPro IPR030678, Peptide/Ni-bd IPR039424, SBP_5 IPR000914, SBP_5_dom |
PANTHERi | PTHR30290, PTHR30290, 1 hit |
Pfami | View protein in Pfam PF00496, SBP_bac_5, 1 hit |
PIRSFi | PIRSF002741, MppA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YGIS_ECOLI | |
Accessioni | Q46863Primary (citable) accession number: Q46863 Secondary accession number(s): Q2M9I0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families