UniProtKB - Q46851 (GPR_ECOLI)
Protein
L-glyceraldehyde 3-phosphate reductase
Gene
gpr
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the stereospecific, NADPH-dependent reduction of L-glyceraldehyde 3-phosphate (L-GAP). The physiological role of gpr is the detoxification of L-GAP, which may be formed by non-enzymatic racemization of GAP. Also involved in the stress response as a methylglyoxal reductase which converts the toxic metabolite methylglyoxal to acetol in vitro and in vivo.3 Publications
Kineticsi
- KM=1.06 mM for 4-nitrobenzaldehyde (at pH 7 and at 25 degrees Celsius)1 Publication
- KM=3.4 mM for methylglyoxal (at pH 7 and at 25 degrees Celsius)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 61 | Important for catalysis | 1 | |
Sitei | 66 | Important for catalysis | 1 | |
Sitei | 97 | Important for catalysis | 1 | |
Sitei | 138 | Important for catalysis | 1 |
GO - Molecular functioni
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: EcoCyc
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- methylglyoxal catabolic process Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Enzyme and pathway databases
BioCyci | EcoCyc:G7558-MONOMER MetaCyc:G7558-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:gpr Synonyms:mgrA, yghZ Ordered Locus Names:b3001, JW2970 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene show 23% decrease in the amount of acetol.
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201331 | 1 – 346 | L-glyceraldehyde 3-phosphate reductaseAdd BLAST | 346 |
Proteomic databases
jPOSTi | Q46851 |
PaxDbi | Q46851 |
PRIDEi | Q46851 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4261416, 10 interactors 851798, 2 interactors |
DIPi | DIP-36026N |
IntActi | Q46851, 6 interactors |
STRINGi | 511145.b3001 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q46851 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the shaker potassium channel beta subunit family.Curated
Phylogenomic databases
eggNOGi | COG0667, Bacteria |
HOGENOMi | CLU_023205_2_0_6 |
InParanoidi | Q46851 |
PhylomeDBi | Q46851 |
Family and domain databases
CDDi | cd06660, Aldo_ket_red, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
SUPFAMi | SSF51430, SSF51430, 1 hit |
i Sequence
Sequence statusi: Complete.
Q46851-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVWLANPERY GQMQYRYCGK SGLRLPALSL GLWHNFGHVN ALESQRAILR
60 70 80 90 100
KAFDLGITHF DLANNYGPPP GSAEENFGRL LREDFAAYRD ELIISTKAGY
110 120 130 140 150
DMWPGPYGSG GSRKYLLASL DQSLKRMGLE YVDIFYSHRV DENTPMEETA
160 170 180 190 200
SALAHAVQSG KALYVGISSY SPERTQKMVE LLREWKIPLL IHQPSYNLLN
210 220 230 240 250
RWVDKSGLLD TLQNNGVGCI AFTPLAQGLL TGKYLNGIPQ DSRMHREGNK
260 270 280 290 300
VRGLTPKMLT EANLNSLRLL NEMAQQRGQS MAQMALSWLL KDDRVTSVLI
310 320 330 340
GASRAEQLEE NVQALNNLTF STKELAQIDQ HIADGELNLW QASSDK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69168.1 U00096 Genomic DNA Translation: AAC76037.1 AP009048 Genomic DNA Translation: BAE77060.1 |
PIRi | G65086 |
RefSeqi | NP_417474.1, NC_000913.3 WP_000262172.1, NZ_SSZK01000023.1 |
Genome annotation databases
EnsemblBacteriai | AAC76037; AAC76037; b3001 BAE77060; BAE77060; BAE77060 |
GeneIDi | 57729174 947480 |
KEGGi | ecj:JW2970 eco:b3001 |
PATRICi | fig|1411691.4.peg.3727 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69168.1 U00096 Genomic DNA Translation: AAC76037.1 AP009048 Genomic DNA Translation: BAE77060.1 |
PIRi | G65086 |
RefSeqi | NP_417474.1, NC_000913.3 WP_000262172.1, NZ_SSZK01000023.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3N6Q | X-ray | 1.80 | A/B/C/D/E/F/G/H | 1-346 | [»] | |
4AST | X-ray | 2.38 | A/B/C/D/E/F/G/H | 1-346 | [»] | |
4AUB | X-ray | 2.05 | A/B/C/D/E/F/G/H | 1-346 | [»] | |
SMRi | Q46851 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261416, 10 interactors 851798, 2 interactors |
DIPi | DIP-36026N |
IntActi | Q46851, 6 interactors |
STRINGi | 511145.b3001 |
Proteomic databases
jPOSTi | Q46851 |
PaxDbi | Q46851 |
PRIDEi | Q46851 |
Genome annotation databases
EnsemblBacteriai | AAC76037; AAC76037; b3001 BAE77060; BAE77060; BAE77060 |
GeneIDi | 57729174 947480 |
KEGGi | ecj:JW2970 eco:b3001 |
PATRICi | fig|1411691.4.peg.3727 |
Organism-specific databases
EchoBASEi | EB2831 |
Phylogenomic databases
eggNOGi | COG0667, Bacteria |
HOGENOMi | CLU_023205_2_0_6 |
InParanoidi | Q46851 |
PhylomeDBi | Q46851 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7558-MONOMER MetaCyc:G7558-MONOMER |
Miscellaneous databases
PROi | PR:Q46851 |
Family and domain databases
CDDi | cd06660, Aldo_ket_red, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
SUPFAMi | SSF51430, SSF51430, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GPR_ECOLI | |
Accessioni | Q46851Primary (citable) accession number: Q46851 Secondary accession number(s): Q2M9J6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 27, 2002 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families