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UniProtKB - Q46821 (UACT_ECOLI)

Entry version 144 (25 May 2022)
Sequence version 2 (30 May 2000)
Previous versions | rss
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Protein

Uric acid transporter UacT

Gene

uacT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Proton-dependent high-capacity transporter for uric acid. Shows also a low capacity for transport of xanthine at 37 degrees Celsius but not at 25 degrees Celsius.

1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited in the presence of the protonophore carbonyl cyanide m-chlorophenyl hydrazone.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.5 mM for uric acid1 Publication
  2. KM=0.3 mM for xanthine1 Publication
  1. Vmax=715 nmol/min/mg enzyme with acid uric as substrate1 Publication
  2. Vmax=14 nmol/min/mg enzyme with xanthine as substrate (at 37 degrees Celsius)1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • symporter activity Source: UniProtKB-KW
  • urate transmembrane transporter activity Source: EcoCyc
  • xanthine transmembrane transporter activity Source: EcoCyc
Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processHydrogen ion transport, Ion transport, Symport, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:YGFU-MONOMER

Protein family/group databases

Transport Classification Database

More...TCDBi		2.A.40.3.3, the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Uric acid transporter UacT
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:uacT
Synonyms:ygfU
Ordered Locus Names:b2888, JW5470
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 29CytoplasmicSequence analysisAdd BLAST29
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei30 – 50HelicalSequence analysisAdd BLAST21
Topological domaini51 – 62PeriplasmicSequence analysisAdd BLAST12
Transmembranei63 – 83HelicalSequence analysisAdd BLAST21
Topological domaini84 – 92CytoplasmicSequence analysis9
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 115PeriplasmicSequence analysis2
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Topological domaini137 – 142CytoplasmicSequence analysis6
Transmembranei143 – 163HelicalSequence analysisAdd BLAST21
Topological domaini164 – 178PeriplasmicSequence analysisAdd BLAST15
Transmembranei179 – 199HelicalSequence analysisAdd BLAST21
Topological domaini200 – 204CytoplasmicSequence analysis5
Transmembranei205 – 225HelicalSequence analysisAdd BLAST21
Topological domaini226 – 261PeriplasmicSequence analysisAdd BLAST36
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Topological domaini283 – 337CytoplasmicSequence analysisAdd BLAST55
Transmembranei338 – 358HelicalSequence analysisAdd BLAST21
Topological domaini359PeriplasmicSequence analysis1
Transmembranei360 – 380HelicalSequence analysisAdd BLAST21
Topological domaini381 – 392CytoplasmicSequence analysisAdd BLAST12
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 421PeriplasmicSequence analysis8
Transmembranei422 – 442HelicalSequence analysisAdd BLAST21
Topological domaini443 – 482CytoplasmicSequence analysisAdd BLAST40

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi37H → K or L: Lack of activity. 1 Publication1
Mutagenesisi37H → N: Strong decrease in activity. 1 Publication1
Mutagenesisi100T → A: Decrease in activity with uric acid. Highly active with xanthine. 1 Publication1
Mutagenesisi100T → S: Decrease in activity. 1 Publication1
Mutagenesisi256S → Q: Almost no change in activity. 1 Publication1
Mutagenesisi259T → V: Almost no change in activity. 1 Publication1
Mutagenesisi270E → D, N or Q: Lack of activity. 1 Publication1
Mutagenesisi278L → T: Almost no change in activity. 1 Publication1
Mutagenesisi298D → E: Strong decrease in activity. 1 Publication1
Mutagenesisi298D → N: Lack of activity. 1 Publication1
Mutagenesisi317S → A: Decrease in activity. 1 Publication1
Mutagenesisi318Q → E or N: Strong decrease in activity. 1 Publication1
Mutagenesisi319N → D or Q: Lack of activity. 1 Publication1
Mutagenesisi320V → N: Lack of activity. 1 Publication1
Mutagenesisi327R → G: Almost no change in activity. 1 Publication1
Mutagenesisi426S → N: Almost no change in activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001659681 – 482Uric acid transporter UacTAdd BLAST482

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q46821

PRoteomics IDEntifications database

More...PRIDEi		Q46821

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4262336, 163 interactors

Database of interacting proteins

More...DIPi		DIP-12176N

STRING: functional protein association networks

More...STRINGi		511145.b2888

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		Q46821

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q46821

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG2233, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_017959_8_2_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q46821

Identification of Orthologs from Complete Genome Data

More...OMAi		WANEMPL

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q46821

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR006043, NCS2
IPR017588, UacT-like
IPR006042, Xan_ur_permease

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00860, Xan_ur_permease, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00801, ncs2, 1 hit
TIGR03173, pbuX, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS01116, XANTH_URACIL_PERMASE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q46821-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSAIDSQLPS SSGQDRPTDE VDRILSPGKL IILGLQHVLV MYAGAVAVPL 
        60         70         80         90        100
MIGDRLGLSK EAIAMLISSD LFCCGIVTLL QCIGIGRFMG IRLPVIMSVT 
       110        120        130        140        150
FAAVTPMIAI GMNPDIGLLG IFGATIAAGF ITTLLAPLIG RLMPLFPPLV 
       160        170        180        190        200
TGVVITSIGL SIIQVGIDWA AGGKGNPQYG NPVYLGISFA VLIFILLITR 
       210        220        230        240        250
YAKGFMSNVA VLLGIVFGFL LSWMMNEVNL SGLHDASWFA IVTPMSFGMP 
       260        270        280        290        300
IFDPVSILTM TAVLIIVFIE SMGMFLALGE IVGRKLSSHD IIRGLRVDGV 
       310        320        330        340        350
GTMIGGTFNS FPHTSFSQNV GLVSVTRVHS RWVCISSGII LILFGMVPKM 
       360        370        380        390        400
AVLVASIPQF VLGGAGLVMF GMVLATGIRI LSRCNYTTNR YNLYIVAISL 
       410        420        430        440        450
GVGMTPTLSH DFFSKLPAVL QPLLHSGIML ATLSAVVLNV FFNGYQHHAD 
       460        470        480 
LVKESVSDKD LKVRTVRMWL LMRKLKKNEH GE
Length:482
Mass (Da):51,758
Last modified:May 30, 2000 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE08EF1D4CBD9D066
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA83069 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U28375 Genomic DNA Translation: AAA83069.1 Different initiation.
U00096 Genomic DNA Translation: AAC75926.2
AP009048 Genomic DNA Translation: BAE76953.1

Protein sequence database of the Protein Information Resource

More...PIRi		H65072

NCBI Reference Sequences

More...RefSeqi		NP_417364.2, NC_000913.3
WP_001295374.1, NZ_SSZK01000003.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC75926; AAC75926; b2888
BAE76953; BAE76953; BAE76953

Database of genes from NCBI RefSeq genomes

More...GeneIDi		66673240
949017

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW5470
eco:b2888

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.3847

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA Translation: AAA83069.1 Different initiation.
U00096 Genomic DNA Translation: AAC75926.2
AP009048 Genomic DNA Translation: BAE76953.1
PIRiH65072
RefSeqiNP_417364.2, NC_000913.3
WP_001295374.1, NZ_SSZK01000003.1

3D structure databases

AlphaFoldDBiQ46821
SMRiQ46821
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4262336, 163 interactors
DIPiDIP-12176N
STRINGi511145.b2888

Protein family/group databases

TCDBi2.A.40.3.3, the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family

Proteomic databases

PaxDbiQ46821
PRIDEiQ46821

Protocols and materials databases

The DNASU plasmid repository

More...DNASUi		949017

Genome annotation databases

EnsemblBacteriaiAAC75926; AAC75926; b2888
BAE76953; BAE76953; BAE76953
GeneIDi66673240
949017
KEGGiecj:JW5470
eco:b2888
PATRICifig|1411691.4.peg.3847

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB2882

Phylogenomic databases

eggNOGiCOG2233, Bacteria
HOGENOMiCLU_017959_8_2_6
InParanoidiQ46821
OMAiWANEMPL
PhylomeDBiQ46821

Enzyme and pathway databases

BioCyciEcoCyc:YGFU-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:Q46821

Family and domain databases

InterProiView protein in InterPro
IPR006043, NCS2
IPR017588, UacT-like
IPR006042, Xan_ur_permease
PfamiView protein in Pfam
PF00860, Xan_ur_permease, 1 hit
TIGRFAMsiTIGR00801, ncs2, 1 hit
TIGR03173, pbuX, 1 hit
PROSITEiView protein in PROSITE
PS01116, XANTH_URACIL_PERMASE, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiUACT_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q46821
Secondary accession number(s): Q2M9V3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: May 25, 2022
This is version 144 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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