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UniProtKB - Q46821 (UACT_ECOLI)
Protein
Uric acid transporter UacT
Gene
uacT
Organism
Escherichia coli (strain K12)
Status
Functioni
Proton-dependent high-capacity transporter for uric acid. Shows also a low capacity for transport of xanthine at 37 degrees Celsius but not at 25 degrees Celsius.
1 PublicationActivity regulationi
Inhibited in the presence of the protonophore carbonyl cyanide m-chlorophenyl hydrazone.1 Publication
Kineticsi
- KM=0.5 mM for uric acid1 Publication
- KM=0.3 mM for xanthine1 Publication
- Vmax=715 nmol/min/mg enzyme with acid uric as substrate1 Publication
- Vmax=14 nmol/min/mg enzyme with xanthine as substrate (at 37 degrees Celsius)1 Publication
GO - Molecular functioni
- symporter activity Source: UniProtKB-KW
- urate transmembrane transporter activity Source: EcoCyc
- xanthine transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- urate transport Source: EcoCyc
- xanthine transport Source: EcoCyc
Keywordsi
Biological process | Hydrogen ion transport, Ion transport, Symport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:YGFU-MONOMER |
Protein family/group databases
TCDBi | 2.A.40.3.3, the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family |
Names & Taxonomyi
Protein namesi | Recommended name: Uric acid transporter UacT |
Gene namesi | Name:uacT Synonyms:ygfU Ordered Locus Names:b2888, JW5470 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 29 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Transmembranei | 30 – 50 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 51 – 62 | PeriplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 63 – 83 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 84 – 92 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 93 – 113 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 114 – 115 | PeriplasmicSequence analysis | 2 | |
Transmembranei | 116 – 136 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 137 – 142 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 143 – 163 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 164 – 178 | PeriplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 179 – 199 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 200 – 204 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 205 – 225 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 226 – 261 | PeriplasmicSequence analysisAdd BLAST | 36 | |
Transmembranei | 262 – 282 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 283 – 337 | CytoplasmicSequence analysisAdd BLAST | 55 | |
Transmembranei | 338 – 358 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 359 | PeriplasmicSequence analysis | 1 | |
Transmembranei | 360 – 380 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 381 – 392 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 393 – 413 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 414 – 421 | PeriplasmicSequence analysis | 8 | |
Transmembranei | 422 – 442 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 443 – 482 | CytoplasmicSequence analysisAdd BLAST | 40 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 37 | H → K or L: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 37 | H → N: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 100 | T → A: Decrease in activity with uric acid. Highly active with xanthine. 1 Publication | 1 | |
Mutagenesisi | 100 | T → S: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 256 | S → Q: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 259 | T → V: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 270 | E → D, N or Q: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 278 | L → T: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 298 | D → E: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 298 | D → N: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 317 | S → A: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 318 | Q → E or N: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 319 | N → D or Q: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 320 | V → N: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 327 | R → G: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 426 | S → N: Almost no change in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000165968 | 1 – 482 | Uric acid transporter UacTAdd BLAST | 482 |
Proteomic databases
PaxDbi | Q46821 |
PRIDEi | Q46821 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262336, 163 interactors |
DIPi | DIP-12176N |
STRINGi | 511145.b2888 |
Family & Domainsi
Sequence similaritiesi
Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2233, Bacteria |
HOGENOMi | CLU_017959_8_2_6 |
InParanoidi | Q46821 |
OMAi | WANEMPL |
PhylomeDBi | Q46821 |
Family and domain databases
InterProi | View protein in InterPro IPR006043, NCS2 IPR017588, UacT-like IPR006042, Xan_ur_permease |
Pfami | View protein in Pfam PF00860, Xan_ur_permease, 1 hit |
TIGRFAMsi | TIGR00801, ncs2, 1 hit TIGR03173, pbuX, 1 hit |
PROSITEi | View protein in PROSITE PS01116, XANTH_URACIL_PERMASE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q46821-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAIDSQLPS SSGQDRPTDE VDRILSPGKL IILGLQHVLV MYAGAVAVPL
60 70 80 90 100
MIGDRLGLSK EAIAMLISSD LFCCGIVTLL QCIGIGRFMG IRLPVIMSVT
110 120 130 140 150
FAAVTPMIAI GMNPDIGLLG IFGATIAAGF ITTLLAPLIG RLMPLFPPLV
160 170 180 190 200
TGVVITSIGL SIIQVGIDWA AGGKGNPQYG NPVYLGISFA VLIFILLITR
210 220 230 240 250
YAKGFMSNVA VLLGIVFGFL LSWMMNEVNL SGLHDASWFA IVTPMSFGMP
260 270 280 290 300
IFDPVSILTM TAVLIIVFIE SMGMFLALGE IVGRKLSSHD IIRGLRVDGV
310 320 330 340 350
GTMIGGTFNS FPHTSFSQNV GLVSVTRVHS RWVCISSGII LILFGMVPKM
360 370 380 390 400
AVLVASIPQF VLGGAGLVMF GMVLATGIRI LSRCNYTTNR YNLYIVAISL
410 420 430 440 450
GVGMTPTLSH DFFSKLPAVL QPLLHSGIML ATLSAVVLNV FFNGYQHHAD
460 470 480
LVKESVSDKD LKVRTVRMWL LMRKLKKNEH GE
Sequence cautioni
The sequence AAA83069 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28375 Genomic DNA Translation: AAA83069.1 Different initiation. U00096 Genomic DNA Translation: AAC75926.2 AP009048 Genomic DNA Translation: BAE76953.1 |
PIRi | H65072 |
RefSeqi | NP_417364.2, NC_000913.3 WP_001295374.1, NZ_SSZK01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC75926; AAC75926; b2888 BAE76953; BAE76953; BAE76953 |
GeneIDi | 66673240 949017 |
KEGGi | ecj:JW5470 eco:b2888 |
PATRICi | fig|1411691.4.peg.3847 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28375 Genomic DNA Translation: AAA83069.1 Different initiation. U00096 Genomic DNA Translation: AAC75926.2 AP009048 Genomic DNA Translation: BAE76953.1 |
PIRi | H65072 |
RefSeqi | NP_417364.2, NC_000913.3 WP_001295374.1, NZ_SSZK01000003.1 |
3D structure databases
AlphaFoldDBi | Q46821 |
SMRi | Q46821 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262336, 163 interactors |
DIPi | DIP-12176N |
STRINGi | 511145.b2888 |
Protein family/group databases
TCDBi | 2.A.40.3.3, the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family |
Proteomic databases
PaxDbi | Q46821 |
PRIDEi | Q46821 |
Protocols and materials databases
DNASUi | 949017 |
Genome annotation databases
EnsemblBacteriai | AAC75926; AAC75926; b2888 BAE76953; BAE76953; BAE76953 |
GeneIDi | 66673240 949017 |
KEGGi | ecj:JW5470 eco:b2888 |
PATRICi | fig|1411691.4.peg.3847 |
Organism-specific databases
EchoBASEi | EB2882 |
Phylogenomic databases
eggNOGi | COG2233, Bacteria |
HOGENOMi | CLU_017959_8_2_6 |
InParanoidi | Q46821 |
OMAi | WANEMPL |
PhylomeDBi | Q46821 |
Enzyme and pathway databases
BioCyci | EcoCyc:YGFU-MONOMER |
Miscellaneous databases
PROi | PR:Q46821 |
Family and domain databases
InterProi | View protein in InterPro IPR006043, NCS2 IPR017588, UacT-like IPR006042, Xan_ur_permease |
Pfami | View protein in Pfam PF00860, Xan_ur_permease, 1 hit |
TIGRFAMsi | TIGR00801, ncs2, 1 hit TIGR03173, pbuX, 1 hit |
PROSITEi | View protein in PROSITE PS01116, XANTH_URACIL_PERMASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UACT_ECOLI | |
Accessioni | Q46821Primary (citable) accession number: Q46821 Secondary accession number(s): Q2M9V3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 30, 2000 | |
Last modified: | May 25, 2022 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families