UniProtKB - Q46811 (YGFK_ECOLI)
Protein
Putative oxidoreductase YgfK
Gene
ygfK
Organism
Escherichia coli (strain K12)
Status
Functioni
Could be an iron-sulfur flavoprotein with NADPH:O2 oxidoreductase activity.1 Publication
Cofactori
[4Fe-4S] clusterPROSITE-ProRule annotationNote: Binds 1 [4Fe-4S] cluster.PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 938 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 941 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 944 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 948 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- glutamate synthase activity Source: GO_Central
- iron ion binding Source: EcoCyc
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- ammonia assimilation cycle Source: GO_Central
- glutamate biosynthetic process Source: GO_Central
Keywordsi
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G7497-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Putative oxidoreductase YgfKCuratedAlternative name(s): Putative oxidoreductase Fe-S subunit1 Publication |
Gene namesi | Name:ygfK Ordered Locus Names:b2878, JW5923 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Insertion mutant is unable to reduce selenate to selenium.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201329 | 1 – 1032 | Putative oxidoreductase YgfKAdd BLAST | 1032 |
Proteomic databases
PaxDbi | Q46811 |
PRIDEi | Q46811 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262325, 21 interactors 853310, 1 interactor |
DIPi | DIP-28091N |
IntActi | Q46811, 2 interactors |
STRINGi | 511145.b2878 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 928 – 958 | 4Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 31 |
Phylogenomic databases
eggNOGi | COG0493, Bacteria COG1145, Bacteria |
HOGENOMi | CLU_014791_0_0_6 |
Family and domain databases
Gene3Di | 1.10.1060.10, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR028261, DPD_II IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR009051, Helical_ferredxn IPR017701, Se_rdtase_YgfK |
Pfami | View protein in Pfam PF14691, Fer4_20, 1 hit PF07992, Pyr_redox_2, 1 hit |
SUPFAMi | SSF46548, SSF46548, 2 hits |
TIGRFAMsi | TIGR03315, Se_ygfK, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q46811-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGDIMRPIPF EELLTRIFDE YQQQRSIFGI PEQQFYSPVK GKTVSVFGET
60 70 80 90 100
CATPVGPAAG PHTQLAQNIV TSWLTGGRFI ELKTVQILDR LELEKPCIDA
110 120 130 140 150
EDECFNTEWS TEFTLLKAWD EYLKAWFALH LLEAMFQPSD SGKSFIFNMS
160 170 180 190 200
VGYNLEGIKQ PPMQQFIDNM MDASDHPKFA QYRDTLNKLL QDDAFLARHG
210 220 230 240 250
LQEKRESLQA LPARIPTSMV HGVTLSTMHG CPPHEIEAIC RYMLEEKGLN
260 270 280 290 300
TFVKLNPTLL GYARVREILD VCGFGYIGLK EESFDHDLKL TQALEMLERL
310 320 330 340 350
MALAKEKSLG FGVKLTNTLG TINNKGALPG EEMYMSGRAL FPLSINVAAV
360 370 380 390 400
LSRAFDGKLP ISYSGGASQL TIRDIFDTGI RPITMATDLL KPGGYLRLSA
410 420 430 440 450
CMRELEGSDA WGLDHVDVER LNRLAADALT MEYTQKHWKP EERIEVAEDL
460 470 480 490 500
PLTDCYVAPC VTACAIKQDI PEYIRLLGEH RYADALELIY QRNALPAITG
510 520 530 540 550
HICDHQCQYN CTRLDYDSAL NIRELKKVAL EKGWDEYKQR WHKPAGSGSR
560 570 580 590 600
HPVAVIGAGP AGLAAGYFLA RAGHPVTLFE REANAGGVVK NIIPQFRIPA
610 620 630 640 650
ELIQHDIDFV AAHGVKFEYG CSPDLTIEQL KNQGFHYVLI ATGTDKNSGV
660 670 680 690 700
KLAGDNQNVW KSLPFLREYN KGTALKLGKH VVVVGAGNTA MDCARAALRV
710 720 730 740 750
PGVEKATIVY RRSLQEMPAW REEYEEALHD GVEFRFLNNP ERFDADGTLT
760 770 780 790 800
LRVMSLGEPD EKGRRRPVET NETVTLLVDS LITAIGEQQD TEALNAMGVP
810 820 830 840 850
LDKNGWPDVD HNGETRLTDV FMIGDVQRGP SSIVAAVGTA RRATDAILSR
860 870 880 890 900
ENIRSHQNDK YWNNVNPAEI YQRKGDISIT LVNSDDRDAF VAQEAARCLE
910 920 930 940 950
CNYVCSKCVD VCPNRANVSI AVPGFQNRFQ TLHLDAYCNE CGNCAQFCPW
960 970 980 990 1000
NGKPYKDKIT VFSLAQDFDN SSNPGFLVED CRVRVRLNNQ SWVLNIDSKG
1010 1020 1030
QFNNVPPELN DMCRIISHVH QHHHYLLGRV EV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28375 Genomic DNA Translation: AAA83059.1 U00096 Genomic DNA Translation: AAC75916.1 AP009048 Genomic DNA Translation: BAE76944.1 |
PIRi | F65071 |
RefSeqi | NP_417354.1, NC_000913.3 WP_000502395.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75916; AAC75916; b2878 BAE76944; BAE76944; BAE76944 |
GeneIDi | 949068 |
KEGGi | ecj:JW5923 eco:b2878 |
PATRICi | fig|1411691.4.peg.3856 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28375 Genomic DNA Translation: AAA83059.1 U00096 Genomic DNA Translation: AAC75916.1 AP009048 Genomic DNA Translation: BAE76944.1 |
PIRi | F65071 |
RefSeqi | NP_417354.1, NC_000913.3 WP_000502395.1, NZ_LN832404.1 |
3D structure databases
SMRi | Q46811 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262325, 21 interactors 853310, 1 interactor |
DIPi | DIP-28091N |
IntActi | Q46811, 2 interactors |
STRINGi | 511145.b2878 |
Proteomic databases
PaxDbi | Q46811 |
PRIDEi | Q46811 |
Genome annotation databases
EnsemblBacteriai | AAC75916; AAC75916; b2878 BAE76944; BAE76944; BAE76944 |
GeneIDi | 949068 |
KEGGi | ecj:JW5923 eco:b2878 |
PATRICi | fig|1411691.4.peg.3856 |
Organism-specific databases
EchoBASEi | EB2873 |
Phylogenomic databases
eggNOGi | COG0493, Bacteria COG1145, Bacteria |
HOGENOMi | CLU_014791_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7497-MONOMER |
Miscellaneous databases
PROi | PR:Q46811 |
Family and domain databases
Gene3Di | 1.10.1060.10, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR028261, DPD_II IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR009051, Helical_ferredxn IPR017701, Se_rdtase_YgfK |
Pfami | View protein in Pfam PF14691, Fer4_20, 1 hit PF07992, Pyr_redox_2, 1 hit |
SUPFAMi | SSF46548, SSF46548, 2 hits |
TIGRFAMsi | TIGR03315, Se_ygfK, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YGFK_ECOLI | |
Accessioni | Q46811Primary (citable) accession number: Q46811 Secondary accession number(s): Q2M9W2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 27, 2002 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |