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Entry version 140 (02 Dec 2020)
Sequence version 2 (16 Nov 2001)
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Protein

D-phenylhydantoinase

Gene

hyuA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives with an aromatic side chains at the 5'-position. Has no activity on dihydropyrimidines. The physiological function is unknown.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Zn2+Curated1 Publication, Ni2+Curated1 Publication, Co2+Curated1 Publication, Mn2+Curated1 PublicationNote: Binds 2 divalent metal cations per subunit. Can use zinc, nickel, cobalt or manganese.Curated1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Hydantoin derivatives with an aliphatic or no side chain at their 5'-position results in a much lower level of activity than those with aromatic side chains at the 5'-position.
  1. KM=7.8 mM for phenylhydantoin1 Publication
  2. KM=32.8 mM for hydroxyphenylhydantoin1 Publication
  3. KM=138 mM for hydantoin1 Publication
  1. Vmax=12.6 µmol/min/mg enzyme with hydroxyphenylhydantoin as substrate1 Publication
  2. Vmax=3.3 µmol/min/mg enzyme with phenylhydantoin as substrate1 Publication
  3. Vmax=0.15 µmol/min/mg enzyme with hydantoin as substrate1 Publication

pH dependencei

Optimum pH is 8-8.5.1 Publication

Temperature dependencei

Optimum temperature is 45-50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi59Divalent metal cation 1By similarity1
Metal bindingi61Divalent metal cation 1By similarity1
Metal bindingi151Divalent metal cation 1; via carbamate groupBy similarity1
Metal bindingi151Divalent metal cation 2; via carbamate groupBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei156SubstrateBy similarity1
Metal bindingi182Divalent metal cation 2By similarity1
Metal bindingi239Divalent metal cation 2By similarity1
Binding sitei286Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Metal bindingi313Divalent metal cation 1By similarity1
Binding sitei335Substrate; via carbonyl oxygenBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase
LigandMetal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:G7492-MONOMER
MetaCyc:G7492-MONOMER

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
Q46806

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
D-phenylhydantoinase (EC:3.5.2.-1 Publication)
Alternative name(s):
Hydantoin-utilizing enzyme HyuA
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:hyuA
Synonyms:ygeZ
Ordered Locus Names:b2873, JW2841
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001659371 – 461D-phenylhydantoinaseAdd BLAST461

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei151N6-carboxylysineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Carboxylation allows a single lysine to coordinate two divalent metal cations.By similarity

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q46806

PRoteomics IDEntifications database

More...
PRIDEi
Q46806

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homotetramer.

1 Publication

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4261249, 13 interactors

Protein interaction database and analysis system

More...
IntActi
Q46806, 2 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b2873

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q46806

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0044, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_015572_2_0_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q46806

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q46806

Family and domain databases

Conserved Domains Database

More...
CDDi
cd01314, D-HYD, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.30.40.10, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_01644, D_hydantoinase, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006680, Amidohydro-rel
IPR023766, D_phenylhydantoinase
IPR011778, Hydantoinase/dihydroPyrase
IPR011059, Metal-dep_hydrolase_composite
IPR032466, Metal_Hydrolase

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01979, Amidohydro_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51338, SSF51338, 2 hits
SSF51556, SSF51556, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02033, D-hydantoinase, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q46806-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRVLIKNGTV VNADGQAKQD LLIESGIVRQ LGNNISPQLP YEEIDATGCY
60 70 80 90 100
VFPGGVDVHT HFNIDVGIAR SCDDFFTGTR AAACGGTTTI IDHMGFGPNG
110 120 130 140 150
CRLRHQLEVY RGYAAHKAVI DYSFHGVIQH INHAILDEIP MIVEEGLSSF
160 170 180 190 200
KLYLTYQYKL NDDEVLQALR RLHESGALTT VHPENDAAIA SKRAEFIAAG
210 220 230 240 250
LTAPRYHALS RPLECEAEAI ARMINLAQIA GNAPLYIVHL SNGLGLDYLR
260 270 280 290 300
LARANHQPVW VETCPQYLLL DERSYDTEDG MKFILSPPLR NVREQDKLWC
310 320 330 340 350
GISDGAIDVV ATDHCTFSMA QRLQISKGDF SRCPNGLPGV ENRMQLLFSS
360 370 380 390 400
GVMTGRITPE RFVELTSAMP ARLFGLWPQK GLLAPGSDGD VVIIDPRQSQ
410 420 430 440 450
QIQHRHLHDN ADYSPWEGFT CQGAIVRTLS RGETIFCDGT FTGKAGRGRF
460
LRRKPFVPPV L
Length:461
Mass (Da):51,025
Last modified:November 16, 2001 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i5525E62DB7F8DB5B
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA83054 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U28375 Genomic DNA Translation: AAA83054.1 Different initiation.
U00096 Genomic DNA Translation: AAC75911.2
AP009048 Genomic DNA Translation: BAE76939.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A65071

NCBI Reference Sequences

More...
RefSeqi
NP_417349.4, NC_000913.3
WP_001264442.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC75911; AAC75911; b2873
BAE76939; BAE76939; BAE76939

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
947359

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW2841
eco:b2873

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.3861

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA Translation: AAA83054.1 Different initiation.
U00096 Genomic DNA Translation: AAC75911.2
AP009048 Genomic DNA Translation: BAE76939.1
PIRiA65071
RefSeqiNP_417349.4, NC_000913.3
WP_001264442.1, NZ_LN832404.1

3D structure databases

SMRiQ46806
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4261249, 13 interactors
IntActiQ46806, 2 interactors
STRINGi511145.b2873

Proteomic databases

PaxDbiQ46806
PRIDEiQ46806

Genome annotation databases

EnsemblBacteriaiAAC75911; AAC75911; b2873
BAE76939; BAE76939; BAE76939
GeneIDi947359
KEGGiecj:JW2841
eco:b2873
PATRICifig|1411691.4.peg.3861

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB2868

Phylogenomic databases

eggNOGiCOG0044, Bacteria
HOGENOMiCLU_015572_2_0_6
InParanoidiQ46806
PhylomeDBiQ46806

Enzyme and pathway databases

BioCyciEcoCyc:G7492-MONOMER
MetaCyc:G7492-MONOMER
SABIO-RKiQ46806

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q46806

Family and domain databases

CDDicd01314, D-HYD, 1 hit
Gene3Di2.30.40.10, 1 hit
HAMAPiMF_01644, D_hydantoinase, 1 hit
InterProiView protein in InterPro
IPR006680, Amidohydro-rel
IPR023766, D_phenylhydantoinase
IPR011778, Hydantoinase/dihydroPyrase
IPR011059, Metal-dep_hydrolase_composite
IPR032466, Metal_Hydrolase
PfamiView protein in Pfam
PF01979, Amidohydro_1, 1 hit
SUPFAMiSSF51338, SSF51338, 2 hits
SSF51556, SSF51556, 1 hit
TIGRFAMsiTIGR02033, D-hydantoinase, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPHYDA_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q46806
Secondary accession number(s): Q2M9W7
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 16, 2001
Last modified: December 2, 2020
This is version 140 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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