UniProtKB - Q46082 (ANFD_RUMHU)
Protein
Nitrogenase iron-iron protein alpha chain
Gene
anfD
Organism
Ruminiclostridium hungatei (Clostridium hungatei)
Status
Functioni
This iron-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation. Other nitrogenase complexes utilize a molybdenum-iron protein or a vanadium-iron protein.
Miscellaneous
The structure of the 8Fe-9S-C-homocitryl cluster is assumed to be analogous to the 7Fe-Mo-9S-C-homocitryl cluster.
Catalytic activityi
- 16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H+ + H2 + 2 NH4+ + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphateEC:1.18.6.1
Cofactori
Protein has several cofactor binding sites:- [8Fe-7S] clusterBy similarityNote: Binds 1 [8Fe-7S] cluster per heterodimer.By similarity
- [8Fe-9S-C-homocitryl] clusterBy similarityNote: Binds 1 [8Fe-9S-C-homocitryl] cluster per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 49 | Iron-sulfur (8Fe-7S); shared with beta chainBy similarity | 1 | |
Metal bindingi | 75 | Iron-sulfur (8Fe-7S); shared with beta chainBy similarity | 1 | |
Metal bindingi | 257 | Iron-sulfur-carbon (8Fe-9S-C-homocitryl)By similarity | 1 | |
Metal bindingi | 423 | Iron-sulfur-carbon (8Fe-9S-C-homocitryl); via pros nitrogenBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- carbonyl sulfide nitrogenase activity Source: UniProtKB-EC
- iron-sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- nitrogenase activity Source: UniProtKB-EC
GO - Biological processi
- nitrogen fixation Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Nitrogen fixation |
Ligand | ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Nitrogenase iron-iron protein alpha chain (EC:1.18.6.1)Alternative name(s): Dinitrogenase 3 subunit alpha Nitrogenase component I |
Gene namesi | Name:anfD |
Organismi | Ruminiclostridium hungatei (Clostridium hungatei) |
Taxonomic identifieri | 48256 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Clostridia › Clostridiales › Hungateiclostridiaceae › Ruminiclostridium |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000153063 | 1 – 518 | Nitrogenase iron-iron protein alpha chainAdd BLAST | 518 |
Interactioni
Subunit structurei
Hexamer of two alpha, two beta, and two delta chains.
By similarityFamily & Domainsi
Family and domain databases
InterProi | View protein in InterPro IPR000510, Nase/OxRdtase_comp1 IPR005974, Nase_asu IPR010143, Nase_comp1_asu IPR000318, Nase_comp1_CS IPR011290, Nase_Fe-Fe_asu |
PANTHERi | PTHR43457, PTHR43457, 1 hit |
Pfami | View protein in Pfam PF00148, Oxidored_nitro, 1 hit |
TIGRFAMsi | TIGR01284, alt_nitrog_alph, 1 hit TIGR01861, ANFD, 1 hit TIGR01862, N2-ase-Ialpha, 1 hit |
PROSITEi | View protein in PROSITE PS00699, NITROGENASE_1_1, 1 hit |
i Sequence
Sequence statusi: Complete.
Q46082-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPHHEFECSK VIPERKKHAV IKGKGETLAD ALPQGYLNTI PGSISERGCA
60 70 80 90 100
YCVAKHVIGT PMKDVIHISH GPVGCTYDTW QTKRYISDND NFQLKYTYAT
110 120 130 140 150
DVKEKHIVFG AEKLLKQNII EAFKRFPQIK RMTIYQTPCT ALIGDDINAI
160 170 180 190 200
AEEVMEEMPE VDIFVCNSPG FAGPSQSGGH HKINIAWINQ KVGTVEPEIT
210 220 230 240 250
GDHVINYVGE YNIQGDQEVM VDYFKRMGIQ VLSTFTGNLS YDGLRAMHRA
260 270 280 290 300
HLNVLECARS AEYICNELRV RYGIPRLDID GFGFKPLADS LRKYGMFFGI
310 320 330 340 350
EDRRKAIIDE EVARWKPELD WYKERLMGKK VSVWPGGSKL WHWAHVIEEE
360 370 380 390 400
MGLKVVSVYT KFGHQGDMEK GMPRCGEGTL AIDDPNELEG LEALEMLKPD
410 420 430 440 450
IILTGKRPGE VAKKVRVPYL NAHAYHNGPY KGFEGWVRFA RDIYNAIYSP
460 470 480 490 500
IHQLSGIDIT KDNAPEWGNG FRTRQMLSDG NLSDAVRNSE TLAQYTGGYD
510
SVSNVREREY PAFERKVG
Sequence cautioni
The sequence AAB02935 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U59415 Genomic DNA Translation: AAB02935.1 Frameshift. |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U59415 Genomic DNA Translation: AAB02935.1 Frameshift. |
3D structure databases
SMRi | Q46082 |
ModBasei | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR000510, Nase/OxRdtase_comp1 IPR005974, Nase_asu IPR010143, Nase_comp1_asu IPR000318, Nase_comp1_CS IPR011290, Nase_Fe-Fe_asu |
PANTHERi | PTHR43457, PTHR43457, 1 hit |
Pfami | View protein in Pfam PF00148, Oxidored_nitro, 1 hit |
TIGRFAMsi | TIGR01284, alt_nitrog_alph, 1 hit TIGR01861, ANFD, 1 hit TIGR01862, N2-ase-Ialpha, 1 hit |
PROSITEi | View protein in PROSITE PS00699, NITROGENASE_1_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ANFD_RUMHU | |
Accessioni | Q46082Primary (citable) accession number: Q46082 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | December 20, 2005 | |
Last modified: | October 7, 2020 | |
This is version 87 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |