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Protein

Chondroitinase-B

Gene

cslB

Organism
Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves the glycosaminoglycan, dermatan sulfate.

Catalytic activityi

Eliminative cleavage of dermatan sulfate containing (1->4)-beta-D-hexosaminyl and (1->3)-beta-D-glucurosonyl or (1->3)-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups to yield a 4,5-unsaturated dermatan-sulfate disaccharide (Delta-UA-GalNAc-4S).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2501
Active sitei2721
Active sitei3331

GO - Molecular functioni

Keywordsi

Molecular functionLyase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15800
PHEP485917:G1GFH-804-MONOMER
BRENDAi4.2.2.19 2286
SABIO-RKiQ46079

Protein family/group databases

CAZyiPL6 Polysaccharide Lyase Family 6

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitinase-B (EC:4.2.2.19)
Alternative name(s):
Chondroitin sulfate B lyase
Chondroitin-B eliminase
Chondroitin-B lyase
Gene namesi
Name:cslB
Ordered Locus Names:Phep_0789
OrganismiPedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3)
Taxonomic identifieri485917 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesSphingobacteriiaSphingobacterialesSphingobacteriaceaePedobacter
Proteomesi
  • UP000000852 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi250K → A: Complete loss of activity. 1 Publication1
Mutagenesisi272H → A: Partial loss of activity. 1 Publication1
Mutagenesisi333E → A: Partial loss of activity. 1 Publication1
Mutagenesisi363R → A: No effect. 1 Publication1
Mutagenesisi364R → A: Partial loss of activity and altered product profile. 1 Publication1

Chemistry databases

DrugBankiDB04492 2-(Acetylamino)-2-Deoxy-4-O-Sulfo-Alpha-D-Galactopyranose
DB03863 2-O-Methyl Fucose
DB04548 4-Deoxy-D-Glucuronic Acid
DB04515 6-Deoxy-2-O-Methyl-Alpha-L-Galactopyranose
DB01872 Acetylgalactosamine-4-Sulfate
DB03389 alpha-D-Xylopyranose
DB02379 Beta-D-Glucose
DB03088 Pyroglutamic Acid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Add BLAST25
ChainiPRO_000002492826 – 506Chondroitinase-BAdd BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26Pyrrolidone carboxylic acid1 Publication1
Glycosylationi234O-linked (Man...) serine1

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PRIDEiQ46079

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi485917.Phep_0789

Structurei

Secondary structure

1506
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiQ46079
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ46079

Family & Domainsi

Sequence similaritiesi

Belongs to the polysaccharide lyase 8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK19053
OMAiRNDIGRC
OrthoDBiPOG091H03H8

Family and domain databases

CDDicd14251 PL-6, 1 hit
Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR039513 PL-6
PfamiView protein in Pfam
PF14592 Chondroitinas_B, 1 hit
SUPFAMiSSF51126 SSF51126, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q46079-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKMLNKLAGY LLPIMVLLNV APCLGQVVAS NETLYQVVKE VKPGGLVQIA
60 70 80 90 100
DGTYKDVQLI VSNSGKSGLP ITIKALNPGK VFFTGDAKVE LRGEHLILEG
110 120 130 140 150
IWFKDGNRAI QAWKSHGPGL VAIYGSYNRI TACVFDCFDE ANSAYITTSL
160 170 180 190 200
TEDGKVPQHC RIDHCSFTDK ITFDQVINLN NTARAIKDGS VGGPAMYHRV
210 220 230 240 250
DHCFFSNPQK PGNAGGGIRI GYYRNDIGRC LVDSNLFMRQ DSEAEIITSK
260 270 280 290 300
SQENVYYGNT YLNCQGTMNF RHGDHQVAIN NFYIGNDQRF GYGGMFVWGS
310 320 330 340 350
RHVIACNYFE LSETIKSRGN AALYLNPGAM ASEHALAFDM LIANNAFINV
360 370 380 390 400
NGYAIHFNPL DERRKEYCAA NRLKFETPHQ LMLKGNLFFK DKPYVYPFFK
410 420 430 440 450
DDYFIAGKNS WTGNVALGVE KGIPVNISAN RSAYKPVKIK DIQPIEGIAL
460 470 480 490 500
DLNALISKGI TGKPLSWDEV RPYWLKEMPG TYALTARLSA DRAAKFKAVI

KRNKEH
Length:506
Mass (Da):56,337
Last modified:November 3, 2009 - v2
Checksum:i454B93EC0AACD2A3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195A → G in AAC83384 (PubMed:10618199).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27584 Genomic DNA Translation: AAC83384.1
CP001681 Genomic DNA Translation: ACU03011.1
RefSeqiWP_012780957.1, NZ_AQGK01000003.1

Genome annotation databases

EnsemblBacteriaiACU03011; ACU03011; Phep_0789
KEGGiphe:Phep_0789

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27584 Genomic DNA Translation: AAC83384.1
CP001681 Genomic DNA Translation: ACU03011.1
RefSeqiWP_012780957.1, NZ_AQGK01000003.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DBGX-ray1.70A1-506[»]
1DBOX-ray1.70A1-506[»]
1OFLX-ray1.70A27-506[»]
1OFMX-ray1.80A27-506[»]
SMRiQ46079
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi485917.Phep_0789

Chemistry databases

DrugBankiDB04492 2-(Acetylamino)-2-Deoxy-4-O-Sulfo-Alpha-D-Galactopyranose
DB03863 2-O-Methyl Fucose
DB04548 4-Deoxy-D-Glucuronic Acid
DB04515 6-Deoxy-2-O-Methyl-Alpha-L-Galactopyranose
DB01872 Acetylgalactosamine-4-Sulfate
DB03389 alpha-D-Xylopyranose
DB02379 Beta-D-Glucose
DB03088 Pyroglutamic Acid

Protein family/group databases

CAZyiPL6 Polysaccharide Lyase Family 6

Proteomic databases

PRIDEiQ46079

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACU03011; ACU03011; Phep_0789
KEGGiphe:Phep_0789

Phylogenomic databases

KOiK19053
OMAiRNDIGRC
OrthoDBiPOG091H03H8

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15800
PHEP485917:G1GFH-804-MONOMER
BRENDAi4.2.2.19 2286
SABIO-RKiQ46079

Miscellaneous databases

EvolutionaryTraceiQ46079

Family and domain databases

CDDicd14251 PL-6, 1 hit
Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR039513 PL-6
PfamiView protein in Pfam
PF14592 Chondroitinas_B, 1 hit
SUPFAMiSSF51126 SSF51126, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCSLB_PEDHD
AccessioniPrimary (citable) accession number: Q46079
Secondary accession number(s): C6Y218
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: November 3, 2009
Last modified: October 10, 2018
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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