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UniProtKB - Q46079 (CSLB_PEDHD)
Protein
Chondroitinase-B
Gene
cslB
Organism
Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3)
Status
Functioni
Cleaves the glycosaminoglycan, dermatan sulfate.
Catalytic activityi
- Eliminative cleavage of dermatan sulfate containing (1->4)-beta-D-hexosaminyl and (1->3)-beta-D-glucurosonyl or (1->3)-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups to yield a 4,5-unsaturated dermatan-sulfate disaccharide (Delta-UA-GalNAc-4S). EC:4.2.2.19
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 250 | 1 | ||
Active sitei | 272 | 1 | ||
Active sitei | 333 | 1 |
GO - Molecular functioni
- chondroitin B lyase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Lyase |
Enzyme and pathway databases
BRENDAi | 4.2.2.19, 2286 |
SABIO-RKi | Q46079 |
Protein family/group databases
CAZyi | PL6, Polysaccharide Lyase Family 6 |
Names & Taxonomyi
Protein namesi | Recommended name: Chondroitinase-B (EC:4.2.2.19)Alternative name(s): Chondroitin sulfate B lyase Chondroitin-B eliminase Chondroitin-B lyase |
Gene namesi | Name:cslB Ordered Locus Names:Phep_0789 |
Organismi | Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) |
Taxonomic identifieri | 485917 [NCBI] |
Taxonomic lineagei | Bacteria › Bacteroidetes › Sphingobacteriia › Sphingobacteriales › Sphingobacteriaceae › Pedobacter › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 250 | K → A: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 272 | H → A: Partial loss of activity. 1 Publication | 1 | |
Mutagenesisi | 333 | E → A: Partial loss of activity. 1 Publication | 1 | |
Mutagenesisi | 363 | R → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 364 | R → A: Partial loss of activity and altered product profile. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB04492, 2-(acetylamino)-2-deoxy-4-O-sulfo-alpha-D-galactopyranose DB01872, 2-deoxy-2-acetamido-beta-D-galactose-4-sulfate DB04515, 2-O-methyl-alpha-L-fucopyranose DB03863, 2-O-Methyl-beta-L-fucopyranose DB04548, 4-Deoxy-D-Glucuronic Acid DB03389, alpha-D-Xylopyranose DB02379, Beta-D-Glucose |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Add BLAST | 25 | |
ChainiPRO_0000024928 | 26 – 506 | Chondroitinase-BAdd BLAST | 481 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 26 | Pyrrolidone carboxylic acid1 Publication | 1 | |
Glycosylationi | 234 | O-linked (Man...) serine | 1 |
Keywords - PTMi
Glycoprotein, Pyrrolidone carboxylic acidInteractioni
Subunit structurei
Monomer.
Protein-protein interaction databases
STRINGi | 485917.Phep_0789 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q46079 |
SMRi | Q46079 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q46079 |
Family & Domainsi
Sequence similaritiesi
Belongs to the polysaccharide lyase 8 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3420, Bacteria |
HOGENOMi | CLU_531940_0_0_10 |
OMAi | RNDIGRC |
OrthoDBi | 1055328at2 |
Family and domain databases
CDDi | cd14251, PL-6, 1 hit |
Gene3Di | 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR039513, PL-6 |
Pfami | View protein in Pfam PF14592, Chondroitinas_B, 1 hit |
SUPFAMi | SSF51126, SSF51126, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q46079-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKMLNKLAGY LLPIMVLLNV APCLGQVVAS NETLYQVVKE VKPGGLVQIA
60 70 80 90 100
DGTYKDVQLI VSNSGKSGLP ITIKALNPGK VFFTGDAKVE LRGEHLILEG
110 120 130 140 150
IWFKDGNRAI QAWKSHGPGL VAIYGSYNRI TACVFDCFDE ANSAYITTSL
160 170 180 190 200
TEDGKVPQHC RIDHCSFTDK ITFDQVINLN NTARAIKDGS VGGPAMYHRV
210 220 230 240 250
DHCFFSNPQK PGNAGGGIRI GYYRNDIGRC LVDSNLFMRQ DSEAEIITSK
260 270 280 290 300
SQENVYYGNT YLNCQGTMNF RHGDHQVAIN NFYIGNDQRF GYGGMFVWGS
310 320 330 340 350
RHVIACNYFE LSETIKSRGN AALYLNPGAM ASEHALAFDM LIANNAFINV
360 370 380 390 400
NGYAIHFNPL DERRKEYCAA NRLKFETPHQ LMLKGNLFFK DKPYVYPFFK
410 420 430 440 450
DDYFIAGKNS WTGNVALGVE KGIPVNISAN RSAYKPVKIK DIQPIEGIAL
460 470 480 490 500
DLNALISKGI TGKPLSWDEV RPYWLKEMPG TYALTARLSA DRAAKFKAVI
KRNKEH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 195 | A → G in AAC83384 (PubMed:10618199).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27584 Genomic DNA Translation: AAC83384.1 CP001681 Genomic DNA Translation: ACU03011.1 |
RefSeqi | WP_012780957.1, NZ_AQGK01000003.1 |
Genome annotation databases
EnsemblBacteriai | ACU03011; ACU03011; Phep_0789 |
KEGGi | phe:Phep_0789 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27584 Genomic DNA Translation: AAC83384.1 CP001681 Genomic DNA Translation: ACU03011.1 |
RefSeqi | WP_012780957.1, NZ_AQGK01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DBG | X-ray | 1.70 | A | 1-506 | [»] | |
1DBO | X-ray | 1.70 | A | 1-506 | [»] | |
1OFL | X-ray | 1.70 | A | 27-506 | [»] | |
1OFM | X-ray | 1.80 | A | 27-506 | [»] | |
AlphaFoldDBi | Q46079 | |||||
SMRi | Q46079 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 485917.Phep_0789 |
Chemistry databases
DrugBanki | DB04492, 2-(acetylamino)-2-deoxy-4-O-sulfo-alpha-D-galactopyranose DB01872, 2-deoxy-2-acetamido-beta-D-galactose-4-sulfate DB04515, 2-O-methyl-alpha-L-fucopyranose DB03863, 2-O-Methyl-beta-L-fucopyranose DB04548, 4-Deoxy-D-Glucuronic Acid DB03389, alpha-D-Xylopyranose DB02379, Beta-D-Glucose |
Protein family/group databases
CAZyi | PL6, Polysaccharide Lyase Family 6 |
Genome annotation databases
EnsemblBacteriai | ACU03011; ACU03011; Phep_0789 |
KEGGi | phe:Phep_0789 |
Phylogenomic databases
eggNOGi | COG3420, Bacteria |
HOGENOMi | CLU_531940_0_0_10 |
OMAi | RNDIGRC |
OrthoDBi | 1055328at2 |
Enzyme and pathway databases
BRENDAi | 4.2.2.19, 2286 |
SABIO-RKi | Q46079 |
Miscellaneous databases
EvolutionaryTracei | Q46079 |
Family and domain databases
CDDi | cd14251, PL-6, 1 hit |
Gene3Di | 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR039513, PL-6 |
Pfami | View protein in Pfam PF14592, Chondroitinas_B, 1 hit |
SUPFAMi | SSF51126, SSF51126, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CSLB_PEDHD | |
Accessioni | Q46079Primary (citable) accession number: Q46079 Secondary accession number(s): C6Y218 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 15, 2003 |
Last sequence update: | November 3, 2009 | |
Last modified: | May 25, 2022 | |
This is version 113 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families