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UniProtKB - Q45477 (SYI_BACSU)
Protein
Isoleucine--tRNA ligase
Gene
ileS
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).
UniRule annotationCatalytic activityi
- EC:6.1.1.5UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 553 | Isoleucyl-adenylateUniRule annotation | 1 | |
Binding sitei | 597 | ATPUniRule annotation | 1 |
GO - Molecular functioni
- aminoacyl-tRNA editing activity Source: InterPro
- ATP binding Source: UniProtKB-UniRule
- isoleucine-tRNA ligase activity Source: GO_Central
- tRNA binding Source: InterPro
GO - Biological processi
- isoleucyl-tRNA aminoacylation Source: GO_Central
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU15430-MONOMER |
BRENDAi | 6.1.1.5, 658 |
SABIO-RKi | Q45477 |
Names & Taxonomyi
Protein namesi | Recommended name: Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)Alternative name(s): Isoleucyl-tRNA synthetaseUniRule annotation Short name: IleRSUniRule annotation |
Gene namesi | Name:ileSUniRule annotation Ordered Locus Names:BSU15430 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098351 | 1 – 921 | Isoleucine--tRNA ligaseAdd BLAST | 921 |
Proteomic databases
jPOSTi | Q45477 |
PaxDbi | Q45477 |
PRIDEi | Q45477 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationProtein-protein interaction databases
IntActi | Q45477, 1 interactor |
MINTi | Q45477 |
STRINGi | 224308.BSU15430 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 57 – 67 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 594 – 598 | 'KMSKS' region | 5 |
Domaini
IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0060, Bacteria |
InParanoidi | Q45477 |
OMAi | HLGTAWN |
PhylomeDBi | Q45477 |
Family and domain databases
CDDi | cd07960, Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 2 hits |
HAMAPi | MF_02002, Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033708, Anticodon_Ile_BEm IPR002301, Ile-tRNA-ligase IPR023585, Ile-tRNA-ligase_type1 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
Q45477-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDFKDTLLMP KTDFPMRGNL PNREPDIQKK WEEEDIYRLV QERTKDRPKF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KILKDFIVRY KSMSGYNAPY VPGWDTHGLP
110 120 130 140 150
IETALTKNKK VNRKEMSVAE FRKLCEEYAW KQIEGQREQF KRLGVRGDWE
160 170 180 190 200
NPYVTLKPEY EAQQIRVFGE MAKRGYIYKG LKPVNWSPSS ESALAEAEIE
210 220 230 240 250
YQDKRSASIY VAFGVKDGKG VLENGERIII WTTTPWTIPA NLGISVHPDL
260 270 280 290 300
EYSVIAVGED RFVVASALVE NVASACGFDQ YEVTRTVKGK DLENIIAEHP
310 320 330 340 350
LYGRDSLVML GEHVTTDAGT GCVHTAPGHG EDDFIIGQKY GLDVLCPVDE
360 370 380 390 400
KGVMTSEAPG FEGMFYDDAN KAITQQLDEK GALVKLEFIT HSYPHDWRTK
410 420 430 440 450
KPTIFRATAQ WFASIKDFRS DLLDAIKETK WVPEWGEQRL HNMVRDRGDW
460 470 480 490 500
CISRQRAWGV PIPVFYAENG EPVITDETIE HVSELFRQHG SNIWFEKEAK
510 520 530 540 550
DLLPEGFTHP GSPNGTFTKE QDIMDVWFDS GSSHQAVLEE RDDLVRPADL
560 570 580 590 600
YLEGSDQYRG WFNSSLSTAV AVTGKAPYKG VLSHGFALDG EGRKMSKSIG
610 620 630 640 650
NVVVPAKVMK QLGADILRLW VSSVDYQADV RVSDAILKQV AEVYRKIRNT
660 670 680 690 700
FRFLHGNLFD FDPKTNAVAV EDLREVDQYM LIKLNKLIDK VKKAYDEYEF
710 720 730 740 750
AVVYHSIHNF CTIELSSFYL DFAKDIVYIE HADHPDRRSM QTVFYETLLA
760 770 780 790 800
LVKLSAPILP HTADELWSHL TFVEEQSVQL TDMPETITVP NSEATEEKFD
810 820 830 840 850
RFMALRDDVL KALETARNEK IIGKSLEANL KLYPNKENKE LLASIKENLS
860 870 880 890 900
QLFIVSELTI SEENEAPNDA QSFATGKIAV EKAEGEMCER SRVISKDVGA
910 920
NPKYPTLSLR NAEIVEKYYQ K
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13417.2 U60901 Genomic DNA Translation: AAB49280.1 U48870 Genomic DNA Translation: AAB57764.1 |
PIRi | H69643 |
RefSeqi | NP_389426.2, NC_000964.3 WP_003245512.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13417; CAB13417; BSU_15430 |
GeneIDi | 940119 |
KEGGi | bsu:BSU15430 |
PATRICi | fig|224308.179.peg.1682 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13417.2 U60901 Genomic DNA Translation: AAB49280.1 U48870 Genomic DNA Translation: AAB57764.1 |
PIRi | H69643 |
RefSeqi | NP_389426.2, NC_000964.3 WP_003245512.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | Q45477 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q45477, 1 interactor |
MINTi | Q45477 |
STRINGi | 224308.BSU15430 |
Proteomic databases
jPOSTi | Q45477 |
PaxDbi | Q45477 |
PRIDEi | Q45477 |
Genome annotation databases
EnsemblBacteriai | CAB13417; CAB13417; BSU_15430 |
GeneIDi | 940119 |
KEGGi | bsu:BSU15430 |
PATRICi | fig|224308.179.peg.1682 |
Phylogenomic databases
eggNOGi | COG0060, Bacteria |
InParanoidi | Q45477 |
OMAi | HLGTAWN |
PhylomeDBi | Q45477 |
Enzyme and pathway databases
BioCyci | BSUB:BSU15430-MONOMER |
BRENDAi | 6.1.1.5, 658 |
SABIO-RKi | Q45477 |
Family and domain databases
CDDi | cd07960, Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 2 hits |
HAMAPi | MF_02002, Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033708, Anticodon_Ile_BEm IPR002301, Ile-tRNA-ligase IPR023585, Ile-tRNA-ligase_type1 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYI_BACSU | |
Accessioni | Q45477Primary (citable) accession number: Q45477 Secondary accession number(s): O31730, P71022 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | June 16, 2009 | |
Last modified: | February 23, 2022 | |
This is version 149 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families