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UniProtKB - Q45477 (SYI_BACSU)

Entry version 149 (23 Feb 2022)
Sequence version 3 (16 Jun 2009)
Previous versions | rss
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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei553Isoleucyl-adenylateUniRule annotation1
Binding sitei597ATPUniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		BSUB:BSU15430-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		6.1.1.5, 658

SABIO-RK: Biochemical Reaction Kinetics Database

More...SABIO-RKi		Q45477

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BSU15430
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000983511 – 921Isoleucine--tRNA ligaseAdd BLAST921

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q45477

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q45477

PRoteomics IDEntifications database

More...PRIDEi		Q45477

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer.

UniRule annotation

Protein-protein interaction databases

Protein interaction database and analysis system

More...IntActi		Q45477, 1 interactor

Molecular INTeraction database

More...MINTi		Q45477

STRING: functional protein association networks

More...STRINGi		224308.BSU15430

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q45477

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi57 – 67'HIGH' regionAdd BLAST11
Motifi594 – 598'KMSKS' region5

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0060, Bacteria

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q45477

Identification of Orthologs from Complete Genome Data

More...OMAi		HLGTAWN

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q45477

Family and domain databases

Conserved Domains Database

More...CDDi		cd07960, Anticodon_Ia_Ile_BEm, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.40.50.620, 2 hits

HAMAP database of protein families

More...HAMAPi		MF_02002, Ile_tRNA_synth_type1, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR001412, aa-tRNA-synth_I_CS
IPR002300, aa-tRNA-synth_Ia
IPR033708, Anticodon_Ile_BEm
IPR002301, Ile-tRNA-ligase
IPR023585, Ile-tRNA-ligase_type1
IPR013155, M/V/L/I-tRNA-synth_anticd-bd
IPR014729, Rossmann-like_a/b/a_fold
IPR009080, tRNAsynth_Ia_anticodon-bd
IPR009008, Val/Leu/Ile-tRNA-synth_edit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF08264, Anticodon_1, 1 hit
PF00133, tRNA-synt_1, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00984, TRNASYNTHILE

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF47323, SSF47323, 1 hit
SSF50677, SSF50677, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00392, ileS, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00178, AA_TRNA_LIGASE_I, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q45477-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDFKDTLLMP KTDFPMRGNL PNREPDIQKK WEEEDIYRLV QERTKDRPKF 
        60         70         80         90        100
VLHDGPPYAN GDIHMGHALN KILKDFIVRY KSMSGYNAPY VPGWDTHGLP 
       110        120        130        140        150
IETALTKNKK VNRKEMSVAE FRKLCEEYAW KQIEGQREQF KRLGVRGDWE 
       160        170        180        190        200
NPYVTLKPEY EAQQIRVFGE MAKRGYIYKG LKPVNWSPSS ESALAEAEIE 
       210        220        230        240        250
YQDKRSASIY VAFGVKDGKG VLENGERIII WTTTPWTIPA NLGISVHPDL 
       260        270        280        290        300
EYSVIAVGED RFVVASALVE NVASACGFDQ YEVTRTVKGK DLENIIAEHP 
       310        320        330        340        350
LYGRDSLVML GEHVTTDAGT GCVHTAPGHG EDDFIIGQKY GLDVLCPVDE 
       360        370        380        390        400
KGVMTSEAPG FEGMFYDDAN KAITQQLDEK GALVKLEFIT HSYPHDWRTK 
       410        420        430        440        450
KPTIFRATAQ WFASIKDFRS DLLDAIKETK WVPEWGEQRL HNMVRDRGDW 
       460        470        480        490        500
CISRQRAWGV PIPVFYAENG EPVITDETIE HVSELFRQHG SNIWFEKEAK 
       510        520        530        540        550
DLLPEGFTHP GSPNGTFTKE QDIMDVWFDS GSSHQAVLEE RDDLVRPADL 
       560        570        580        590        600
YLEGSDQYRG WFNSSLSTAV AVTGKAPYKG VLSHGFALDG EGRKMSKSIG 
       610        620        630        640        650
NVVVPAKVMK QLGADILRLW VSSVDYQADV RVSDAILKQV AEVYRKIRNT 
       660        670        680        690        700
FRFLHGNLFD FDPKTNAVAV EDLREVDQYM LIKLNKLIDK VKKAYDEYEF 
       710        720        730        740        750
AVVYHSIHNF CTIELSSFYL DFAKDIVYIE HADHPDRRSM QTVFYETLLA 
       760        770        780        790        800
LVKLSAPILP HTADELWSHL TFVEEQSVQL TDMPETITVP NSEATEEKFD 
       810        820        830        840        850
RFMALRDDVL KALETARNEK IIGKSLEANL KLYPNKENKE LLASIKENLS 
       860        870        880        890        900
QLFIVSELTI SEENEAPNDA QSFATGKIAV EKAEGEMCER SRVISKDVGA 
       910        920 
NPKYPTLSLR NAEIVEKYYQ K
Length:921
Mass (Da):104,845
Last modified:June 16, 2009 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF41081EA48E317E9
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB13417.2
U60901 Genomic DNA Translation: AAB49280.1
U48870 Genomic DNA Translation: AAB57764.1

Protein sequence database of the Protein Information Resource

More...PIRi		H69643

NCBI Reference Sequences

More...RefSeqi		NP_389426.2, NC_000964.3
WP_003245512.1, NZ_JNCM01000035.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		CAB13417; CAB13417; BSU_15430

Database of genes from NCBI RefSeq genomes

More...GeneIDi		940119

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		bsu:BSU15430

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|224308.179.peg.1682

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB13417.2
U60901 Genomic DNA Translation: AAB49280.1
U48870 Genomic DNA Translation: AAB57764.1
PIRiH69643
RefSeqiNP_389426.2, NC_000964.3
WP_003245512.1, NZ_JNCM01000035.1

3D structure databases

SMRiQ45477
ModBaseiSearch...

Protein-protein interaction databases

IntActiQ45477, 1 interactor
MINTiQ45477
STRINGi224308.BSU15430

Proteomic databases

jPOSTiQ45477
PaxDbiQ45477
PRIDEiQ45477

Genome annotation databases

EnsemblBacteriaiCAB13417; CAB13417; BSU_15430
GeneIDi940119
KEGGibsu:BSU15430
PATRICifig|224308.179.peg.1682

Phylogenomic databases

eggNOGiCOG0060, Bacteria
InParanoidiQ45477
OMAiHLGTAWN
PhylomeDBiQ45477

Enzyme and pathway databases

BioCyciBSUB:BSU15430-MONOMER
BRENDAi6.1.1.5, 658
SABIO-RKiQ45477

Family and domain databases

CDDicd07960, Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_02002, Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412, aa-tRNA-synth_I_CS
IPR002300, aa-tRNA-synth_Ia
IPR033708, Anticodon_Ile_BEm
IPR002301, Ile-tRNA-ligase
IPR023585, Ile-tRNA-ligase_type1
IPR013155, M/V/L/I-tRNA-synth_anticd-bd
IPR014729, Rossmann-like_a/b/a_fold
IPR009080, tRNAsynth_Ia_anticodon-bd
IPR009008, Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264, Anticodon_1, 1 hit
PF00133, tRNA-synt_1, 1 hit
PRINTSiPR00984, TRNASYNTHILE
SUPFAMiSSF47323, SSF47323, 1 hit
SSF50677, SSF50677, 1 hit
TIGRFAMsiTIGR00392, ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178, AA_TRNA_LIGASE_I, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYI_BACSU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q45477
Secondary accession number(s): O31730, P71022
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 16, 2009
Last modified: February 23, 2022
This is version 149 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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