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Protein

Pyruvate kinase

Gene

ttuE

Organism
Agrobacterium vitis (Rhizobium vitis)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno), Enolase (eno), Enolase (eno), Enolase (eno)
  5. Pyruvate kinase (B7W85_22375), Pyruvate kinase (ASB66_20930), Pyruvate kinase (BBK77_18840), Pyruvate kinase (ASB66_16015), Pyruvate kinase (ASB66_06865), Pyruvate kinase (BBK77_07695), Pyruvate kinase (ttuE), Pyruvate kinase (BBK95_25320), Pyruvate kinase (BBK95_17295), Pyruvate kinase (BBK77_10715), Pyruvate kinase (BBK95_21000), Pyruvate kinase (ttuE), Pyruvate kinase (B7W85_13575), Pyruvate kinase (B7W85_04275), Pyruvate kinase (B7W85_21380)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37SubstrateBy similarity1
Metal bindingi39PotassiumBy similarity1
Metal bindingi41PotassiumBy similarity1
Metal bindingi71PotassiumBy similarity1
Sitei220Transition state stabilizerBy similarity1
Metal bindingi222MagnesiumBy similarity1
Binding sitei245Substrate; via amide nitrogenBy similarity1
Metal bindingi246MagnesiumBy similarity1
Binding sitei246Substrate; via amide nitrogenBy similarity1
Binding sitei278SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:ttuE
Encoded oniPlasmid pTrAB40 Publication
OrganismiAgrobacterium vitis (Rhizobium vitis)
Taxonomic identifieri373 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120521 – 482Pyruvate kinaseAdd BLAST482

Proteomic databases

PRIDEiQ44473

Expressioni

Inductioni

By tartrate.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ44473
SMRiQ44473
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q44473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIRNNRRSK IVATVGPASS SPDMLRSLFL AGVDTFRLNF SHGARADHAE
60 70 80 90 100
VYRNIRALEQ EHDAAIAVLQ DLQGPKIRIG VLAHGRLDLA RGSTIGFILG
110 120 130 140 150
REGGEGMNDI PLPHREIFEV AVPGMDLLID DGRIKVRIME VMDGRLVCEV
160 170 180 190 200
LNGGALSNRK GVNVPGAVLD ISPLTAKDRE DLEFGLELGV DWVALSFVQR
210 220 230 240 250
ARDMIEARSL VGDRAGLIAK IEKPSALDDI EDIVRLSDSV MVARGDLGVE
260 270 280 290 300
IPPEDVPGKQ KEIIRACRLA AKPVIVATQM LDSMVSSPTP TRAEASDVAG
310 320 330 340 350
AIYDGADAVM LSAETATGAY PVEAVEIMNR IIEKTEKHKH YRPILEATEP
360 370 380 390 400
DVAQSPPHAV ATAAANVAVA LGSPVVVAYT SSGTTAARIS RARPALPILA
410 420 430 440 450
LTPSEQVARR LNMFWGVVGV RSQDVHTYEA SLIHAQQAVQ EAKLASPSDH
460 470 480
IVIVAGFPFA QQGSTNNLRV VQIAATDNLE IA
Length:482
Mass (Da):51,678
Last modified:November 1, 1996 - v1
Checksum:i2D2B614589CB46E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25634 Genomic DNA Translation: AAA68700.1
RefSeqiWP_071207358.1, NZ_MBFC01000022.1

Similar proteinsi

Entry informationi

Entry nameiKPYK2_AGRVI
AccessioniPrimary (citable) accession number: Q44473
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: February 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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