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Entry version 60 (05 Dec 2018)
Sequence version 2 (03 May 2011)
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Protein

Beta-galactosidase

Gene
N/A
Organism
Arthrobacter sp.
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Highly specific towards beta-D-galactoside substrates. Hydrolyzes 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and o-nitrophenyl-beta-D-galactopyranoside (ONPG). Has activity against p-nitrophenyl(pNP)-beta-D-galactoside, but not significantly at all towards pNP-alpha-D-galactoside, pNP-beta-D-glucoside, pNP-beta-D-mannoside, pNP-beta-L-fucoside, pNP-beta-D-xyloside, pNP-beta-L-arabinoside, pNP-beta-D-galuronide, pNP-beta-D-glucuronide, pNP-beta-D-lactoside or pNP-beta-D-cellobioside.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Activity stimulated by beta-mercaptoethanol.1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.57 mM for ONPG1 Publication
  2. KM=4.81 mM for lactose1 Publication
  1. Vmax=254 µmol/min/mg enzyme with ONPG as substrate1 Publication
  2. Vmax=3.97 µmol/min/mg enzyme with lactose as substrate1 Publication

pH dependencei

Optimum pH is 6.6. Maintains activity over a broad range of pH values from 6 to 9.1 Publication

Temperature dependencei

Optimum temperature is 45-50 degrees Celsius. Activity declines rapidly above 50 degrees Celsius. Stable for at least 70 hours at temperatures 35 degrees Celsius and below. At 50 degrees Celsius loses all activity in less than 15 minutes.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei173SubstrateBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei174Proton donorBy similarity1
Binding sitei345SubstrateBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

Carbohydrate-Active enZymes

More...
CAZyi
GH42 Glycoside Hydrolase Family 42

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-galBy similarity
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArthrobacter sp.
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri1667 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeArthrobacter

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004076801 – 690Beta-galactosidaseAdd BLAST690

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q44233

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence analysis

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.60.40.1180, 1 hit
3.40.50.880, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013739 Beta_galactosidase_C
IPR013738 Beta_galactosidase_Trimer
IPR029062 Class_I_gatase-like
IPR003476 Glyco_hydro_42
IPR013529 Glyco_hydro_42_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF

The PANTHER Classification System

More...
PANTHERi
PTHR36447 PTHR36447, 2 hits

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02449 Glyco_hydro_42, 1 hit
PF08533 Glyco_hydro_42C, 1 hit
PF08532 Glyco_hydro_42M, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF001084 B-galactosidase, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51445 SSF51445, 1 hit
SSF52317 SSF52317, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q44233-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPVPTPLSEG TTPDTAAQEL RTNRLWEALP GLSYGGDYIP NSGRNRSARK
60 70 80 90 100
IYRSCRKPEC RPSALASSPG LGLEPVEGSY DFTWLDEVMD NLAATGIKVA
110 120 130 140 150
LATATAAPPA GWLRKHPEIL PVTAEGSTLG PARAHYLVVG MVLFCRPVCG
160 170 180 190 200
EDDPRLGERY KDHPALALWH VDNELGCHVS EFYGPRRHRR FPSMAEPTLR
210 220 230 240 250
HDRGPQRGLG TAFWSQRYSC FEEILTPRPA PTTLNPTQQL DFQRFSSWGL
260 270 280 290 300
IDFYSMLARG HFARSHPRCP PRQIWWPQAP PCLWDYFDWA KKLECHRQWS
310 320 330 340 350
LPGGRRYRCV TSELAFRRRS DSEAIAGGKP WSPDGALSPC RPCNWLASQH
360 370 380 390 400
DSRTPGEMAR NSLVHVGRGI WMLSCFSSGD RASRVRRNST RPWCRTPEPT
410 420 430 440 450
REYGVKLLSW AQAQSLVRGS RRRGGITHRN RLRLRTLVGK RTGLHPAPMW
460 470 480 490 500
KYLELLRAFH APCSCPASPP IWSIPALTLT AMTWWSSRPC TPSPMPRPAI
510 520 530 540 550
LRQRQNAEPQ CSSATSVDID ENDAVRLGGY PGAFRDLLGV NVEEFHPLPE
560 570 580 590 600
NSTVSLDAGW SGRIWSEHVH LTGAEAKVSF TEAPLTGVPA VTRHAVGTGA
610 620 630 640 650
AWYLATFPDA TGLESLLDSL IAESGVRAPA MAAAGVELSR RSHADGRSYL
660 670 680 690
FAINHNVTEA AVSAQGTELI SGTPFNGTVP AGAVAVIAEG
Length:690
Mass (Da):76,115
Last modified:May 3, 2011 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i83262445ECE19128
GO

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA75601 differs from that shown. Reason: Frameshift at positions 111, 158 and 637.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U17417 Genomic DNA Translation: AAA75601.1 Frameshift.

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17417 Genomic DNA Translation: AAA75601.1 Frameshift.

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein family/group databases

CAZyiGH42 Glycoside Hydrolase Family 42

Proteomic databases

PRIDEiQ44233

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR013739 Beta_galactosidase_C
IPR013738 Beta_galactosidase_Trimer
IPR029062 Class_I_gatase-like
IPR003476 Glyco_hydro_42
IPR013529 Glyco_hydro_42_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR36447 PTHR36447, 2 hits
PfamiView protein in Pfam
PF02449 Glyco_hydro_42, 1 hit
PF08533 Glyco_hydro_42C, 1 hit
PF08532 Glyco_hydro_42M, 1 hit
PIRSFiPIRSF001084 B-galactosidase, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF52317 SSF52317, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBGAL1_ARTSP
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q44233
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: December 5, 2018
This is version 60 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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