UniProtKB - Q43364 (EFTUB_NICSY)
Protein
Elongation factor TuB, chloroplastic
Gene
TUFB
Organism
Nicotiana sylvestris (Wood tobacco) (South American tobacco)
Status
Functioni
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 95 – 102 | GTPBy similarity | 8 | |
Nucleotide bindingi | 157 – 161 | GTPBy similarity | 5 | |
Nucleotide bindingi | 212 – 215 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: InterPro
- GTP binding Source: UniProtKB-KW
- translation elongation factor activity Source: UniProtKB-KW
Keywordsi
Molecular function | Elongation factor |
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor TuB, chloroplasticShort name: EF-TuB |
Gene namesi | Name:TUFB |
Organismi | Nicotiana sylvestris (Wood tobacco) (South American tobacco) |
Taxonomic identifieri | 4096 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana |
Proteomesi |
|
Subcellular locationi
Chloroplast
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 76 | ChloroplastSequence analysisAdd BLAST | 76 | |
ChainiPRO_0000007456 | 77 – 485 | Elongation factor TuB, chloroplasticAdd BLAST | 409 |
Proteomic databases
PRIDEi | Q43364 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 86 – 290 | tr-type GAdd BLAST | 205 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 95 – 102 | G1By similarity | 8 | |
Regioni | 136 – 140 | G2By similarity | 5 | |
Regioni | 157 – 160 | G3By similarity | 4 | |
Regioni | 212 – 215 | G4By similarity | 4 | |
Regioni | 250 – 252 | G5By similarity | 3 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0460, Eukaryota |
Family and domain databases
CDDi | cd01884, EF_Tu, 1 hit cd03697, EFTU_II, 1 hit |
HAMAPi | MF_00118_B, EF_Tu_B, 1 hit |
InterProi | View protein in InterPro IPR041709, EF-Tu_GTP-bd IPR004161, EFTu-like_2 IPR033720, EFTU_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004541, Transl_elong_EFTu/EF1A_bac/org IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00485, EF-Tu, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q43364-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASISAASAT ATASTKLAYP YSPSSSSSSS NTAAVFPSNS SKLILSSSFT
60 70 80 90 100
PTPSTLFLHS PTTTPSTTHP RRFTVRAARG KFERKKPHVN IGTIGHVDHG
110 120 130 140 150
KTTLTAALTM ALASMGNSAP KKYDEIDAAP EERARGITIN TATVEYETEN
160 170 180 190 200
RHYAHVDCPG HADYVKNMIT GAAQMDGAIL VVSGADGPMP QTKEHILLAK
210 220 230 240 250
QVGVPNMVVF LNKQDQVDDE ELLELVELEV RELLSSYEFP GDEIPIISGS
260 270 280 290 300
ALLALEALMA NPSIKRGENQ WVDKIYQLMD NVDEYIPIPQ RQTELPFLMA
310 320 330 340 350
IEDVFSITGR GTVATGRVER GTVKVGEIVD IVGLKDTRNT TVTGVEMFQK
360 370 380 390 400
ILDEAMAGDN VGLLLRGIQK IDIQRGMVLA KPGTITPHTK FEALVYVLKK
410 420 430 440 450
EEGGRHSPFF AGYRPQFYMR TTDVTGKVTV IMSDKGEESK MVMPGDRVNM
460 470 480
VVELIMPVAC EQGMRFAIRE GGKTVGAGVI QKILE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D11470 Genomic DNA Translation: BAA02028.1 D11376 mRNA Translation: BAA01975.1 |
PIRi | S36184 |
RefSeqi | XP_009772722.1, XM_009774420.1 |
Genome annotation databases
GeneIDi | 104223071 |
KEGGi | nsy:104223071 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D11470 Genomic DNA Translation: BAA02028.1 D11376 mRNA Translation: BAA01975.1 |
PIRi | S36184 |
RefSeqi | XP_009772722.1, XM_009774420.1 |
3D structure databases
SMRi | Q43364 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4096.XP_009772722.1 |
Proteomic databases
PRIDEi | Q43364 |
Genome annotation databases
GeneIDi | 104223071 |
KEGGi | nsy:104223071 |
Phylogenomic databases
eggNOGi | KOG0460, Eukaryota |
Family and domain databases
CDDi | cd01884, EF_Tu, 1 hit cd03697, EFTU_II, 1 hit |
HAMAPi | MF_00118_B, EF_Tu_B, 1 hit |
InterProi | View protein in InterPro IPR041709, EF-Tu_GTP-bd IPR004161, EFTu-like_2 IPR033720, EFTU_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004541, Transl_elong_EFTu/EF1A_bac/org IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00485, EF-Tu, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EFTUB_NICSY | |
Accessioni | Q43364Primary (citable) accession number: Q43364 Secondary accession number(s): Q7DNA4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families