UniProtKB - Q43309 (1A14_ARATH)
Protein
1-aminocyclopropane-1-carboxylate synthase 4
Gene
ACS4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene.
Miscellaneous
The stability of ACS proteins, and the regulation of such stability, play a central role in ethylene biosynthesis.
Catalytic activityi
- S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + H+ + S-methyl-5'-thioadenosine1 PublicationEC:4.4.1.141 Publication
Cofactori
Kineticsi
- KM=15 µM for AdoMet
- Vmax=31.20 µM/h/mg enzyme
pH dependencei
Optimum pH is 7.8.
: ethylene biosynthesis via S-adenosyl-L-methionine Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes ethylene from S-adenosyl-L-methionine.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- 1-aminocyclopropane-1-carboxylate synthase 4 (ACS4), 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8), 1-aminocyclopropane-1-carboxylate synthase 9 (ACS9), 1-aminocyclopropane-1-carboxylate synthase 6 (ACS6), 1-aminocyclopropane-1-carboxylate synthase 7 (ACS7), 1-aminocyclopropane-1-carboxylate synthase 2 (ACS2), 1-aminocyclopropane-1-carboxylate synthase 5 (ACS5), 1-aminocyclopropane-1-carboxylate synthase 11 (ACS11)
- 1-aminocyclopropane-1-carboxylate oxidase 5 (At1g77330), 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4), 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1), 1-aminocyclopropane-1-carboxylate oxidase 2 (ACO2), 1-aminocyclopropane-1-carboxylate oxidase 3 (At1g12010)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ethylene from S-adenosyl-L-methionine, the pathway ethylene biosynthesis via S-adenosyl-L-methionine and in Alkene biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 47 | SubstrateBy similarity | 1 | |
Binding sitei | 85 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- 1-aminocyclopropane-1-carboxylate synthase activity Source: TAIR
- identical protein binding Source: IntAct
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- ethylene biosynthetic process Source: UniProtKB-UniPathway
- fruit ripening Source: UniProtKB-KW
Keywordsi
Molecular function | Lyase |
Biological process | Ethylene biosynthesis, Fruit ripening |
Ligand | Pyridoxal phosphate, S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | ARA:AT2G22810-MONOMER MetaCyc:AT2G22810-MONOMER |
SABIO-RKi | Q43309 |
UniPathwayi | UPA00384;UER00562 |
Names & Taxonomyi
Protein namesi | Recommended name: 1-aminocyclopropane-1-carboxylate synthase 4 (EC:4.4.1.14)Short name: ACC synthase 4 Alternative name(s): S-adenosyl-L-methionine methylthioadenosine-lyase 4 |
Gene namesi | Name:ACS4 Synonyms:ACC4 Ordered Locus Names:At2g22810 ORF Names:T20K9.2 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G22810 |
TAIRi | locus:2059170, AT2G22810 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000123898 | 1 – 474 | 1-aminocyclopropane-1-carboxylate synthase 4Add BLAST | 474 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 273 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Post-translational modificationi
Ubiquitinated by XBAT32. Ubiquitination probably leads to its subsequent degradation, thus controlling ethylene production.1 Publication
Keywords - PTMi
Ubl conjugationProteomic databases
PaxDbi | Q43309 |
PRIDEi | Q43309 |
ProteomicsDBi | 244504 |
Expressioni
Tissue specificityi
Expressed in roots, leaves and flowers.1 Publication
Inductioni
By indole-3-acetic acid (IAA) and cycloheximide (CHX). By auxin.2 Publications
Gene expression databases
ExpressionAtlasi | Q43309, baseline and differential |
Genevisiblei | Q43309, AT |
Interactioni
Subunit structurei
Homodimer and heterodimer. In vivo, the relevance of heterodimerization with other ACS enzymes is however unsure (By similarity).
Interacts with XBAT32.
Interacts (via its C-terminal region) with ETO1 and EOL1.
By similarity2 PublicationsBinary interactionsi
Hide detailsQ43309
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-2436015,EBI-2436015 |
ACS6 [Q9SAR0] | 2 | EBI-2436015,EBI-2356658 |
ACS7 [Q9STR4] | 4 | EBI-2436015,EBI-2356842 |
ACS8 [Q9T065] | 2 | EBI-2436015,EBI-2357693 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 2165, 2 interactors |
IntActi | Q43309, 5 interactors |
STRINGi | 3702.AT2G22810.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | KOG0256, Eukaryota |
HOGENOMi | CLU_017584_1_0_1 |
InParanoidi | Q43309 |
OMAi | AACFKRD |
OrthoDBi | 1156861at2759 |
PhylomeDBi | Q43309 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
i Sequence
Sequence statusi: Complete.
Q43309-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVQLSRKATC NSHGQVSSYF LGWEEYEKNP YDVTKNPQGI IQMGLAENQL
60 70 80 90 100
CFDLLESWLA QNTDAACFKR DGQSVFRELA LFQDYHGLSS FKNAFADFMS
110 120 130 140 150
ENRGNRVSFD SNNLVLTAGA TSANETLMFC LADPGDAFLL PTPYYPGFDR
160 170 180 190 200
DLKWRTGVEI VPIQSSSTNG FRITKLALEE AYEQAKKLDL NVKGILITNP
210 220 230 240 250
SNPLGTTTTQ TELNILFDFI TKNKNIHLVS DEIYSGTVFN SSEFISVMEI
260 270 280 290 300
LKNNQLENTD VLNRVHIVCS LSKDLGLPGF RVGAIYSNDK DVISAATKMS
310 320 330 340 350
SFGLVSSQTQ YLLSSLLSDK KFTKNYLREN QKRLKNRQRK LVLGLEAIGI
360 370 380 390 400
KCLKSNAGLF CWVDMRPLLR SKTFEAEMDL WKKIVYEVKL NISPGSSCHC
410 420 430 440 450
EEPGWFRVCF ANMIDETLKL ALKRLKMLVD DENSSRRCQK SKSERLNGSR
460 470
KKTMSNVSNW VFRLSFHDRE AEER
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U23481 mRNA Translation: AAC49037.1 U23482 Genomic DNA Translation: AAA85039.1 AC004786 Genomic DNA Translation: AAC32428.1 AC005617 Genomic DNA Translation: AAM15065.1 CP002685 Genomic DNA Translation: AEC07360.1 AF332404 mRNA Translation: AAG48767.1 |
PIRi | B84617 G46376 |
RefSeqi | NP_179866.1, NM_127846.2 |
Genome annotation databases
EnsemblPlantsi | AT2G22810.1; AT2G22810.1; AT2G22810 |
GeneIDi | 816812 |
Gramenei | AT2G22810.1; AT2G22810.1; AT2G22810 |
KEGGi | ath:AT2G22810 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U23481 mRNA Translation: AAC49037.1 U23482 Genomic DNA Translation: AAA85039.1 AC004786 Genomic DNA Translation: AAC32428.1 AC005617 Genomic DNA Translation: AAM15065.1 CP002685 Genomic DNA Translation: AEC07360.1 AF332404 mRNA Translation: AAG48767.1 |
PIRi | B84617 G46376 |
RefSeqi | NP_179866.1, NM_127846.2 |
3D structure databases
SMRi | Q43309 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 2165, 2 interactors |
IntActi | Q43309, 5 interactors |
STRINGi | 3702.AT2G22810.1 |
Proteomic databases
PaxDbi | Q43309 |
PRIDEi | Q43309 |
ProteomicsDBi | 244504 |
Genome annotation databases
EnsemblPlantsi | AT2G22810.1; AT2G22810.1; AT2G22810 |
GeneIDi | 816812 |
Gramenei | AT2G22810.1; AT2G22810.1; AT2G22810 |
KEGGi | ath:AT2G22810 |
Organism-specific databases
Araporti | AT2G22810 |
TAIRi | locus:2059170, AT2G22810 |
Phylogenomic databases
eggNOGi | KOG0256, Eukaryota |
HOGENOMi | CLU_017584_1_0_1 |
InParanoidi | Q43309 |
OMAi | AACFKRD |
OrthoDBi | 1156861at2759 |
PhylomeDBi | Q43309 |
Enzyme and pathway databases
UniPathwayi | UPA00384;UER00562 |
BioCyci | ARA:AT2G22810-MONOMER MetaCyc:AT2G22810-MONOMER |
SABIO-RKi | Q43309 |
Miscellaneous databases
PROi | PR:Q43309 |
Gene expression databases
ExpressionAtlasi | Q43309, baseline and differential |
Genevisiblei | Q43309, AT |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 1A14_ARATH | |
Accessioni | Q43309Primary (citable) accession number: Q43309 Secondary accession number(s): Q9S9C4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families