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UniProtKB - Q43157 (RPE_SPIOL)
Protein
Ribulose-phosphate 3-epimerase, chloroplastic
Gene
RPE
Organism
Spinacia oleracea (Spinach)
Status
Functioni
Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.
3 PublicationsCatalytic activityi
- EC:5.1.3.13 Publications
Cofactori
Co2+By similarity, Fe2+By similarity, Mn2+By similarity, Zn2+By similarityNote: Binds 1 divalent metal cation per subunit. Active with Co2+, Fe2+, Mn2+ and Zn2+.By similarity
Kineticsi
- KM=250 µM for ribulose 5-phosphate2 Publications
pH dependencei
Optimum pH is 8.5.2 Publications
: Calvin cycle Pathwayi
This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.4 PublicationsView all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 67 | SubstrateBy similarity | 1 | |
Metal bindingi | 92 | Divalent metal cation; via tele nitrogenBy similarity | 1 | |
Active sitei | 94 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 94 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 125 | Divalent metal cation; via pros nitrogenBy similarity | 1 | |
Binding sitei | 125 | SubstrateBy similarity | 1 | |
Active sitei | 236 | Proton donor1 Publication | 1 | |
Metal bindingi | 236 | Divalent metal cationBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ribulose-phosphate 3-epimerase activity Source: UniProtKB
GO - Biological processi
- pentose-phosphate shunt Source: UniProtKB
- reductive pentose-phosphate cycle Source: UniProtKB
Keywordsi
Molecular function | Isomerase |
Biological process | Calvin cycle, Carbohydrate metabolism, Pentose shunt |
Ligand | Cobalt, Iron, Manganese, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 5.1.3.1, 5812 |
SABIO-RKi | Q43157 |
UniPathwayi | UPA00116 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribulose-phosphate 3-epimerase, chloroplastic (EC:5.1.3.13 Publications)Alternative name(s): Pentose-5-phosphate 3-epimerase Short name: PPE R5P3E Short name: RPE |
Gene namesi | Name:RPE |
Organismi | Spinacia oleracea (Spinach) |
Taxonomic identifieri | 3562 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › Caryophyllales › Chenopodiaceae › Chenopodioideae › Anserineae › Spinacia |
Subcellular locationi
Chloroplast
- chloroplast thylakoid membrane 1 Publication
Chloroplast
- chloroplast thylakoid membrane Source: UniProtKB
Keywords - Cellular componenti
Chloroplast, Membrane, Plastid, ThylakoidPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 236 | D → A, E or N: Almost abolished enzyme activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 50 | Chloroplast1 PublicationAdd BLAST | 50 | |
ChainiPRO_0000025416 | 51 – 285 | Ribulose-phosphate 3-epimerase, chloroplasticAdd BLAST | 235 |
Proteomic databases
PRIDEi | Q43157 |
Interactioni
Subunit structurei
Homooctamer.
2 PublicationsFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 203 – 206 | Substrate bindingBy similarity | 4 | |
Regioni | 236 – 238 | Substrate bindingBy similarity | 3 | |
Regioni | 258 – 259 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the ribulose-phosphate 3-epimerase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
OrthoDBi | 1554029at2759 |
Family and domain databases
CDDi | cd00429, RPE, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_02227, RPE, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR026019, Ribul_P_3_epim IPR000056, Ribul_P_3_epim-like IPR011060, RibuloseP-bd_barrel |
PANTHERi | PTHR11749, PTHR11749, 1 hit |
Pfami | View protein in Pfam PF00834, Ribul_P_3_epim, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR01163, rpe, 1 hit |
PROSITEi | View protein in PROSITE PS01085, RIBUL_P_3_EPIMER_1, 1 hit PS01086, RIBUL_P_3_EPIMER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q43157-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAASLCQST LQSQINGFCG GLNIRKLQPS TSSPNSLTFT RRKVQTLVKA
60 70 80 90 100
TSRVDKFSKS DIIVSPSILS ANFAKLGEQV KAVELAGCDW IHVDVMDGRF
110 120 130 140 150
VPNITIGPLV VDALRPVTDL PLDVHLMIVE PEQRVPDFIK AGADIVSVHC
160 170 180 190 200
ELASTIHLHR TVNQIKSLGA KAGVVLNPGT PLSTIEYVLD VVDLVLIMSV
210 220 230 240 250
NPGFGGQSFI ESQVKKISDL RKMCVEKGVN PWIEVDGGVT PANAYKVIEA
260 270 280
GANALVAGSA VFGAKDYAEA IKGIKASKRP EPVAV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42328 mRNA Translation: AAC41677.1 AF070941 mRNA Translation: AAC24708.1 |
PIRi | S62724 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42328 mRNA Translation: AAC41677.1 AF070941 mRNA Translation: AAC24708.1 |
PIRi | S62724 |
3D structure databases
SMRi | Q43157 |
ModBasei | Search... |
Proteomic databases
PRIDEi | Q43157 |
Phylogenomic databases
OrthoDBi | 1554029at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00116 |
BRENDAi | 5.1.3.1, 5812 |
SABIO-RKi | Q43157 |
Family and domain databases
CDDi | cd00429, RPE, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_02227, RPE, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR026019, Ribul_P_3_epim IPR000056, Ribul_P_3_epim-like IPR011060, RibuloseP-bd_barrel |
PANTHERi | PTHR11749, PTHR11749, 1 hit |
Pfami | View protein in Pfam PF00834, Ribul_P_3_epim, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR01163, rpe, 1 hit |
PROSITEi | View protein in PROSITE PS01085, RIBUL_P_3_EPIMER_1, 1 hit PS01086, RIBUL_P_3_EPIMER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RPE_SPIOL | |
Accessioni | Q43157Primary (citable) accession number: Q43157 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | October 7, 2020 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families