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Protein

Pyruvate kinase isozyme A, chloroplastic

Gene
N/A
Organism
Ricinus communis (Castor bean)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
  4. Enolase
  5. Pyruvate kinase isozyme A, chloroplastic, Pyruvate kinase isozyme G, chloroplastic, Pyruvate kinase (RCOM_1122030), Pyruvate kinase (RCOM_1382790), Pyruvate kinase (RCOM_1382780), Pyruvate kinase (RCOM_0840740), Pyruvate kinase (RCOM_0342830), Pyruvate kinase (RCOM_0910580), Pyruvate kinase (RCOM_0865230), Pyruvate kinase (RCOM_1454580), Pyruvate kinase (RCOM_1581420), Pyruvate kinase (RCOM_0248610)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei134SubstrateBy similarity1
Metal bindingi136PotassiumBy similarity1
Metal bindingi168PotassiumBy similarity1
Metal bindingi169Potassium; via carbonyl oxygenBy similarity1
Sitei331Transition state stabilizerBy similarity1
Metal bindingi333MagnesiumSequence analysis1
Binding sitei356Substrate; via amide nitrogenBy similarity1
Metal bindingi357MagnesiumBy similarity1
Binding sitei357Substrate; via amide nitrogenBy similarity1
Binding sitei389SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKiQ43117
UniPathwayi
UPA00109;UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase isozyme A, chloroplastic (EC:2.7.1.40)
OrganismiRicinus communis (Castor bean)
Taxonomic identifieri3988 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 74Chloroplast1 PublicationAdd BLAST74
ChainiPRO_000001666275 – 583Pyruvate kinase isozyme A, chloroplasticAdd BLAST509

Proteomic databases

PRIDEiQ43117

Interactioni

Subunit structurei

Oligomer of alpha and beta subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ43117
SMRiQ43117
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK00873

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform Alpha (identifier: Q43117-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQSLHFSPN LTFAKQPFPK LPLPFPTSNS RYPVNNYKSL SIKASTSPSS
60 70 80 90 100
SSDPQVLVAD NGTGNSGVLY NNNNKSVTVS DPSSIEVDAV TETELKENGF
110 120 130 140 150
RSTRRTKLVC TIGPATCGFE ELEALAVGGM NVARINMCHG TREWHKSVIE
160 170 180 190 200
RVRRLNEEKG FAVAIMMDTE GSEIHMGDLG GASSAKAEDG EIWTFSVRAY
210 220 230 240 250
DSPRPERTIN VNYDGFAEDV KVGDELLVDG GMVRFEVIEK IGPDVKCRCT
260 270 280 290 300
DPGLLLPRAN LTFWRDGSLV RERNAMLPTI SSKDWLDIDF GIAEGVDFIA
310 320 330 340 350
ISFVKSAEVI NHLKSYIAAR SRDSDIAVIA KIESIDSLKN LEEIIRASDG
360 370 380 390 400
AMVARGDLGA QIPLEQVPSA QQNIVQVCRQ LNKPVIVASQ LLESMIEYPT
410 420 430 440 450
PTRAEVADVS EAVRQRADAL MLSGESAMGQ YPEKALAVLR SVSVRIEKWW
460 470 480 490 500
REEKHHEAME LPAIGSTYSD SISEEICNSA AKMANNLGVD ALFVYTKDGH
510 520 530 540 550
MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ WGLIPFRLSF ADDMESNLNK
560 570 580
TFSLLKARGM IKSGDLVIAV SDMLQSIQVM NVP
Length:583
Mass (Da):64,094
Last modified:November 1, 1996 - v1
Checksum:i5DFDE4F778A753C2
GO
Isoform Beta (identifier: Q43117-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-129: MSQSLHFSPN...EELEALAVGG → MAVVVKDLEE...VTAVMGVVGD

Show »
Length:493
Mass (Da):54,462
Checksum:i375858858BF6124F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0028861 – 129MSQSL…LAVGG → MAVVVKDLEEAVRVVVLAVL RDMEVVVVLVTAVMGVVGD in isoform Beta. 1 PublicationAdd BLAST129

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64736 mRNA Translation: AAA33870.1
M64737 mRNA Translation: AAA33871.1
PIRiT10051
T10054
RefSeqiXP_002517044.1, XM_002516998.2 [Q43117-1]

Genome annotation databases

GeneIDi8280200
KEGGircu:8280200

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64736 mRNA Translation: AAA33870.1
M64737 mRNA Translation: AAA33871.1
PIRiT10051
T10054
RefSeqiXP_002517044.1, XM_002516998.2 [Q43117-1]

3D structure databases

ProteinModelPortaliQ43117
SMRiQ43117
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ43117

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi8280200
KEGGircu:8280200

Phylogenomic databases

KOiK00873

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00188

SABIO-RKiQ43117

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKPYA_RICCO
AccessioniPrimary (citable) accession number: Q43117
Secondary accession number(s): Q43118
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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