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Protein

Delta-aminolevulinic acid dehydratase, chloroplastic

Gene

HEMB

Organism
Physcomitrella patens subsp. patens (Moss)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase, chloroplastic (HEMB)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Uroporphyrinogen decarboxylase (PHYPADRAFT_188035), Uroporphyrinogen decarboxylase (PHYPA_023240), Uroporphyrinogen decarboxylase (PHYPA_017203), Uroporphyrinogen decarboxylase (PHYPADRAFT_112870), Uroporphyrinogen decarboxylase (PHYPA_011649)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei300Schiff-base intermediate with substrateBy similarity1
Binding sitei310Substrate 1By similarity1
Binding sitei322Substrate 1By similarity1
Metal bindingi338MagnesiumBy similarity1
Active sitei353Schiff-base intermediate with substrateBy similarity1
Binding sitei379Substrate 2By similarity1
Binding sitei418Substrate 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Lyase
Biological processChlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayi
UPA00251;UER00318

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase, chloroplastic (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:HEMB
Synonyms:ALAD
ORF Names:PHYPADRAFT_221821
OrganismiPhyscomitrella patens subsp. patens (Moss)
Taxonomic identifieri3218 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaBryophytaBryophytinaBryopsidaFunariidaeFunarialesFunariaceaePhyscomitrella

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000013318? – 432Delta-aminolevulinic acid dehydratase, chloroplastic
Transit peptidei1 – ?ChloroplastSequence analysis

Proteomic databases

PRIDEiQ43058

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi3218.PP1S219_94V6.1

Structurei

3D structure databases

SMRiQ43058
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2794 Eukaryota
COG0113 LUCA
HOGENOMiHOG000020323

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR001731 ALAD
IPR030656 ALAD_AS
IPR013785 Aldolase_TIM
PANTHERiPTHR11458 PTHR11458, 1 hit
PfamiView protein in Pfam
PF00490 ALAD, 1 hit
PRINTSiPR00144 DALDHYDRTASE
SMARTiView protein in SMART
SM01004 ALAD, 1 hit
PROSITEiView protein in PROSITE
PS00169 D_ALA_DEHYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43058-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVGVMMAAAA TPGCGVSQAL TACGSHEGLR RVAPVFGPGV VSVSAPCKLP
60 70 80 90 100
RKLNVQAVAE PIAKSSPRTI EECEANVVAG NAPAAPPVPA KPSAPEGTPA
110 120 130 140 150
ISPLVMPARP RRNRRSPALR AAFQETTISP ANFILPLFVH EGEQNAPIGA
160 170 180 190 200
MPGCQRLGWR HGLIDEVYKA RDVGVNSVVL FPKVPDALKS STGDEAYNPD
210 220 230 240 250
GLVPRCIRLL KDKFPDLVIY TDVALDPYSS DGHDGIVRED GLIMNDETVH
260 270 280 290 300
QLCKQAVAQA QAGADVVSPS DMMDGRVGAI RKALDLAGHQ DVSIMAYTAK
310 320 330 340 350
YASAFYGPFR EALDSNPRFG DKKTYQMNPA NYREALIETR MDEAEGADIL
360 370 380 390 400
MVKPAMPYLD VIRLLRDNTA LPISAYQVSG EYSMIRAAAA AGMLDEKKAV
410 420 430
LESLLSIRRA GADVILTYFA IQAAQWLCAE RV
Length:432
Mass (Da):46,263
Last modified:September 3, 2014 - v2
Checksum:iE90018ADAAAA12FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15G → R in CAA61978 (Ref. 1) Curated1
Sequence conflicti24G → S in CAA61978 (Ref. 1) Curated1
Sequence conflicti212 – 214DKF → AI in CAA61978 (Ref. 1) Curated3
Sequence conflicti295M → I in CAA61978 (Ref. 1) Curated1
Sequence conflicti309F → S in CAA61978 (Ref. 1) Curated1
Sequence conflicti388 – 391AAAA → GCR in CAA61978 (Ref. 1) Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89886 mRNA Translation: CAA61978.1
DS545108 Genomic DNA Translation: EDQ57858.1
PIRiS58169
RefSeqiXP_001777328.1, XM_001777276.1
UniGeneiPpa.5213

Genome annotation databases

EnsemblPlantsiPP1S219_94V6.2; PP1S219_94V6.2; PP1S219_94V6
PP1S219_94V6.3; PP1S219_94V6.3; PP1S219_94V6
PP1S219_94V6.4; PP1S219_94V6.4; PP1S219_94V6
GrameneiPP1S219_94V6.2; PP1S219_94V6.2; PP1S219_94V6
PP1S219_94V6.3; PP1S219_94V6.3; PP1S219_94V6
PP1S219_94V6.4; PP1S219_94V6.4; PP1S219_94V6

Similar proteinsi

Entry informationi

Entry nameiHEM2_PHYPA
AccessioniPrimary (citable) accession number: Q43058
Secondary accession number(s): A9TFG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 3, 2014
Last modified: July 18, 2018
This is version 92 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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