Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable 4-coumarate--CoA ligase 2

Gene

4CL2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.

Pathwayi: 3,4',5-trihydroxystilbene biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 3,4',5-trihydroxystilbene from trans-4-coumarate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable 4-coumarate--CoA ligase 3 (4CL3), Probable 4-coumarate--CoA ligase 1 (4CL1), Probable 4-coumarate--CoA ligase 5 (4CL5), Probable 4-coumarate--CoA ligase 4 (4CL4), Probable 4-coumarate--CoA ligase 2 (4CL2)
  2. no protein annotated in this organism
This subpathway is part of the pathway 3,4',5-trihydroxystilbene biosynthesis, which is itself part of Phytoalexin biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3,4',5-trihydroxystilbene from trans-4-coumarate, the pathway 3,4',5-trihydroxystilbene biosynthesis and in Phytoalexin biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei450ATPBy similarity1
Binding sitei465ATPBy similarity1
Binding sitei556ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi219 – 227ATPBy similarity9
Nucleotide bindingi362 – 367ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPhenylpropanoid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.12 4460
UniPathwayi
UPA00372;UER00547

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 4-coumarate--CoA ligase 2 (EC:6.2.1.12)
Short name:
4CL 2
Short name:
Os4CL2
Alternative name(s):
4-coumaroyl-CoA synthase 2
Gene namesi
Name:4CL2
Synonyms:4CL.2
Ordered Locus Names:Os02g0697400, LOC_Os02g46970
ORF Names:P0459B01.4-1, P0666E12.12-1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001930321 – 569Probable 4-coumarate--CoA ligase 2Add BLAST569

Proteomic databases

PaxDbiQ42982
PRIDEiQ42982

Expressioni

Gene expression databases

ExpressionAtlasiQ42982 differential
GenevisibleiQ42982 OS

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os02g46970.1

Structurei

3D structure databases

ProteinModelPortaliQ42982
SMRiQ42982
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni292 – 361SBD1Add BLAST70
Regioni362 – 429SBD2Add BLAST68

Domaini

Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1176 Eukaryota
COG0318 LUCA
HOGENOMiHOG000230009
InParanoidiQ42982
KOiK01904
OMAiWLMQRAF
OrthoDBiEOG093606MC

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Q42982-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MITVAAPEAQ PQVAAAVDEA PPEAVTVFRS KLPDIDIPSH LPLHEYCFAR
60 70 80 90 100
AAELPDAPCL IAAATGRTYT FAETRLLCRR AAAALHRLGV GHGDRVMVLL
110 120 130 140 150
QNCVEFAVAF FAASFLGAVT TAANPFCTPQ EIHKQFKASG VKLILTQSVY
160 170 180 190 200
VDKLRQHEAF PRIDACTVGD DTLTVITIDD DEATPEGCLP FWDLIADADE
210 220 230 240 250
GSVPEVAISP DDPVALPFSS GTTGLPKGVV LTHRSVVSGV AQQVDGENPN
260 270 280 290 300
LHMGAGDVAL CVLPLFHIFS LNSVLLCAVR AGAAVALMPR FEMGAMLGAI
310 320 330 340 350
ERWRVTVAAV VPPLVLALAK NPFVERHDLS SIRIVLSGAA PLGKELEDAL
360 370 380 390 400
RARLPQAIFG QGYGMTEAGP VLSMCPAFAK EPTPAKSGSC GTVVRNAELK
410 420 430 440 450
VVDPDTGFSL GRNLPGEICI RGPQIMKGYL NDPEATAATI DVEGWLHTGD
460 470 480 490 500
IGYVDDDDEV FIVDRVKELI KFKGFQVPPA ELESLLIAHP SIADAAVVPQ
510 520 530 540 550
KDDVAGEVPV AFVVRAADSD ITEESIKEFI SKQVVFYKRL HKVHFIHAIP
560
KSASGKILRR ELRAKLAAC
Length:569
Mass (Da):60,815
Last modified:September 27, 2005 - v2
Checksum:i2A1A1FF3CF7FEC91
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138A → G in AAA69580 (Ref. 1) Curated1
Sequence conflicti186 – 187EG → KA in AAA69580 (Ref. 1) Curated2
Sequence conflicti226P → A in AAA69580 (Ref. 1) Curated1
Sequence conflicti242 – 243QQ → HE in AAA69580 (Ref. 1) Curated2
Sequence conflicti277 – 283CAVRAGA → SRVRPAP in AAA69580 (Ref. 1) Curated7
Sequence conflicti308A → G in AAA69580 (Ref. 1) Curated1
Sequence conflicti316L → V in AAA69580 (Ref. 1) Curated1
Sequence conflicti450D → N in AAA69580 (Ref. 1) Curated1
Sequence conflicti493A → R in AAA69580 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43362 mRNA Translation: AAA69580.1
AP004778 Genomic DNA Translation: BAD07859.1
AP005868 Genomic DNA Translation: BAD08175.1
AP008208 Genomic DNA Translation: BAF09733.1
AP014958 Genomic DNA Translation: BAS80426.1
AK105636 mRNA Translation: BAG97321.1
PIRiT03390
RefSeqiXP_015624111.1, XM_015768625.1
UniGeneiOs.4377

Genome annotation databases

EnsemblPlantsiOs02t0697400-01; Os02t0697400-01; Os02g0697400
GeneIDi4330407
GrameneiOs02t0697400-01; Os02t0697400-01; Os02g0697400
KEGGiosa:4330407

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43362 mRNA Translation: AAA69580.1
AP004778 Genomic DNA Translation: BAD07859.1
AP005868 Genomic DNA Translation: BAD08175.1
AP008208 Genomic DNA Translation: BAF09733.1
AP014958 Genomic DNA Translation: BAS80426.1
AK105636 mRNA Translation: BAG97321.1
PIRiT03390
RefSeqiXP_015624111.1, XM_015768625.1
UniGeneiOs.4377

3D structure databases

ProteinModelPortaliQ42982
SMRiQ42982
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os02g46970.1

Proteomic databases

PaxDbiQ42982
PRIDEiQ42982

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOs02t0697400-01; Os02t0697400-01; Os02g0697400
GeneIDi4330407
GrameneiOs02t0697400-01; Os02t0697400-01; Os02g0697400
KEGGiosa:4330407

Phylogenomic databases

eggNOGiKOG1176 Eukaryota
COG0318 LUCA
HOGENOMiHOG000230009
InParanoidiQ42982
KOiK01904
OMAiWLMQRAF
OrthoDBiEOG093606MC

Enzyme and pathway databases

UniPathwayi
UPA00372;UER00547

BRENDAi6.2.1.12 4460

Gene expression databases

ExpressionAtlasiQ42982 differential
GenevisibleiQ42982 OS

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei4CL2_ORYSJ
AccessioniPrimary (citable) accession number: Q42982
Secondary accession number(s): B7EY74, Q0DYF5, Q6YUH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: September 27, 2005
Last modified: November 7, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again