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UniProtKB - Q42798 (C93A1_SOYBN)
Protein
3,9-dihydroxypterocarpan 6A-monooxygenase
Gene
CYP93A1
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Functioni
Cytochrome P450 involved in the biosynthesis of the phytoalexin glyceollin. Stereospecific for (6aR,11aR)-3,9-dihydroxypterocarpan.
1 PublicationCatalytic activityi
- (6aR,11aR)-3,9-dihydroxypterocarpan + O2 + reduced [NADPH—hemoprotein reductase] = (6aS,11aS)-3,6a,9-trihydroxypterocarpan + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationEC:1.14.14.931 Publication
Cofactori
hemeBy similarity
Kineticsi
- KM=0.1 µM for (6aR,11aR)-3,9-dihydroxypterocarpan1 Publication
pH dependencei
Optimum pH is 7.4.1 Publication
Temperature dependencei
Optimum temperature is 30 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 447 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- 3,9-dihydroxypterocarpan 6a-monooxygenase activity Source: UniProtKB-EC
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: GO_Central
GO - Biological processi
- defense response Source: UniProtKB-KW
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Plant defense |
Ligand | Heme, Iron, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: 3,9-dihydroxypterocarpan 6A-monooxygenase (EC:1.14.14.931 Publication)Alternative name(s): Cytochrome P450 93A1 Dihydroxypterocarpan 6a-hydroxylase Short name: D6aH |
Gene namesi | Name:CYP93A1 Ordered Locus Names:Glyma03g29950 |
Organismi | Glycine max (Soybean) (Glycine hispida) |
Taxonomic identifieri | 3847 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Fabales › Fabaceae › Papilionoideae › 50 kb inversion clade › NPAAA clade › indigoferoid/millettioid clade › Phaseoleae › Glycine › Glycine subgen. Soja |
Proteomesi |
|
Subcellular locationi
Other locations
- Membrane Curated
Other locations
- membrane Source: GO_Central
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000052188 | 1 – 509 | 3,9-dihydroxypterocarpan 6A-monooxygenaseAdd BLAST | 509 |
Expressioni
Inductioni
Up-regulated by methyl jasmonate, elicitor treatments and elicitor peptides GmSubPep, GmPEP890 and GmPEP914.4 Publications
Gene expression databases
Genevisiblei | Q42798, GM |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
HOGENOMi | CLU_001570_4_0_1 |
InParanoidi | Q42798 |
OMAi | HRYDTMM |
OrthoDBi | 702827at2759 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
Q42798-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAYQVLLICL VSTIVFAYIL WRKQSKKNLP PSPKALPIIG HLHLVSPIPH
60 70 80 90 100
QDFYKLSTRH GPIMQLFLGS VPCVVASTAE AAKEFLKTHE INFSNRPGQN
110 120 130 140 150
VAVKGLAYDS QDFLFAFAPF GPYWKFMKKL CMSELLSGRM MDQFLPVRQQ
160 170 180 190 200
ETKRFISRVF RKGVAGEAVD FGDELMTLSN NIVSRMTLSQ KTSENDNQAE
210 220 230 240 250
EMKKLVSNIA ELMGKFNVSD FIWYLKPFDL QGFNRKIKET RDRFDVVVDG
260 270 280 290 300
IIKQRQEERR KNKETGTAKQ FKDMLDVLLD MHEDENAEIK LDKKNIKAFI
310 320 330 340 350
MDIFVAGTDT SAVSIEWAMA ELINNPDVLE KARQEIDAVV GKSRMVEESD
360 370 380 390 400
IANLPYLQAI VRETLRLHPG GPLVVRESSK SAVVCGYDIP AKTRLFVNVW
410 420 430 440 450
AIGRDPNHWE KPFEFRPERF IRDGQNQLDV RGQHYHFIPF GSGRRTCPGA
460 470 480 490 500
SLAWQVVPVN LAIIIQCFQW KLVGGNGKVD MEEKSGITLP RANPIICVPV
PRINPFPTI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83968 mRNA Translation: BAA12159.1 |
PIRi | S62899 |
RefSeqi | NP_001241186.1, NM_001254257.2 |
Genome annotation databases
EnsemblPlantsi | KRH67050; KRH67050; GLYMA_03G143700 |
GeneIDi | 100776878 |
Gramenei | KRH67050; KRH67050; GLYMA_03G143700 |
KEGGi | gmx:100776878 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83968 mRNA Translation: BAA12159.1 |
PIRi | S62899 |
RefSeqi | NP_001241186.1, NM_001254257.2 |
3D structure databases
AlphaFoldDBi | Q42798 |
SMRi | Q42798 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3847.GLYMA03G29950.1 |
Genome annotation databases
EnsemblPlantsi | KRH67050; KRH67050; GLYMA_03G143700 |
GeneIDi | 100776878 |
Gramenei | KRH67050; KRH67050; GLYMA_03G143700 |
KEGGi | gmx:100776878 |
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
HOGENOMi | CLU_001570_4_0_1 |
InParanoidi | Q42798 |
OMAi | HRYDTMM |
OrthoDBi | 702827at2759 |
Gene expression databases
Genevisiblei | Q42798, GM |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | C93A1_SOYBN | |
Accessioni | Q42798Primary (citable) accession number: Q42798 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families