UniProtKB - Q42556 (PMA9_ARATH)
ATPase 9, plasma membrane-type
AHA9
Functioni
The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
Catalytic activityi
- EC:7.1.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 334 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 593 | MagnesiumBy similarity | 1 | |
Metal bindingi | 597 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
- mRNA binding Source: TAIR
- P-type ion transporter activity Source: GO_Central
- P-type proton-exporting transporter activity Source: TAIR
GO - Biological processi
- ion transmembrane transport Source: GO_Central
- proton export across plasma membrane Source: InterPro
- proton transmembrane transport Source: GO_Central
- regulation of intracellular pH Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Hydrogen ion transport, Ion transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT1G80660-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:AHA9 Ordered Locus Names:At1g80660 ORF Names:F23A5.1, T21F11.1 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G80660 |
TAIRi | locus:2025727, AT1G80660 |
Subcellular locationi
Other locations
Mitochondrion
- mitochondrion Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Vacuole
- plant-type vacuole Source: TAIR
Other locations
- integral component of membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 66 | CytoplasmicSequence analysisAdd BLAST | 66 | |
Transmembranei | 67 – 86 | Helical; Name=1Sequence analysisAdd BLAST | 20 | |
Topological domaini | 87 – 98 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 99 – 119 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 120 – 248 | CytoplasmicSequence analysisAdd BLAST | 129 | |
Transmembranei | 249 – 269 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 270 – 278 | ExtracellularSequence analysis | 9 | |
Transmembranei | 279 – 296 | Helical; Name=4Sequence analysisAdd BLAST | 18 | |
Topological domaini | 297 – 648 | CytoplasmicSequence analysisAdd BLAST | 352 | |
Transmembranei | 649 – 670 | Helical; Name=5Sequence analysisAdd BLAST | 22 | |
Topological domaini | 671 – 675 | ExtracellularSequence analysis | 5 | |
Transmembranei | 676 – 698 | Helical; Name=6Sequence analysisAdd BLAST | 23 | |
Topological domaini | 699 – 714 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 715 – 735 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 736 – 756 | ExtracellularSequence analysisAdd BLAST | 21 | |
Transmembranei | 757 – 777 | Helical; Name=8Sequence analysisAdd BLAST | 21 | |
Topological domaini | 778 – 789 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 790 – 810 | Helical; Name=9Sequence analysisAdd BLAST | 21 | |
Topological domaini | 811 – 818 | ExtracellularSequence analysis | 8 | |
Transmembranei | 819 – 839 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Topological domaini | 840 – 954 | CytoplasmicSequence analysisAdd BLAST | 115 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046282 | 1 – 954 | ATPase 9, plasma membrane-typeAdd BLAST | 954 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 886 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 936 | PhosphoserineBy similarity | 1 | |
Modified residuei | 953 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q42556 |
PRIDEi | Q42556 |
PTM databases
iPTMneti | Q42556 |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | Q42556, baseline and differential |
Genevisiblei | Q42556, AT |
Interactioni
Subunit structurei
Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-953. Binding to 14-3-3 proteins activates the H+-ATPase (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 29623, 2 interactors |
STRINGi | 3702.AT1G80660.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 952 – 954 | Interaction with 14-3-3 proteinsBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0205, Eukaryota |
InParanoidi | Q42556 |
PhylomeDBi | Q42556 |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketThis entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGNKDSSWD DIKNEGIDLE KIPIEEVLTQ LRCTREGLTS DEGQTRLEIF
60 70 80 90 100
GPNKLEEKKE NKVLKFLGFM WNPLSWVMEL AAIMAIALAN GGGRPPDWQD
110 120 130 140 150
FVGITVLLII NSTISFIEEN NAGNAAAALM AGLAPKTKVL RDGKWSEQEA
160 170 180 190 200
AILVPGDIIS IKLGDIVPAD GRLLDGDPLK IDQSALTGES LPVTKHPGQE
210 220 230 240 250
VYSGSTCKQG ELEAVVIATG VHTFFGKAAH LVDSTNQEGH FQKVLTAIGN
260 270 280 290 300
FCICSIAIGM LIEIVVMYPI QKRAYRDGID NLLVLLIGGI PIAMPTVLSV
310 320 330 340 350
TMAIGSHRLS QQGAITKRMT AIEEMAGMDV LCSDKTGTLT LNKLTVDKSM
360 370 380 390 400
VEVFVKDLDK DQLLVNAARA SRVENQDAID ACIVGMLGDP REAREGITEV
410 420 430 440 450
HFFPFNPVDK RTAITYIDAN GNWHRVSKGA PEQIIELCNL REDASKRAHD
460 470 480 490 500
IIDKFADRGL RSLAVGRQTV SEKDKNSPGE PWQFLGLLPL FDPPRHDSAE
510 520 530 540 550
TIRRALDLGV NVKMITGDQL AIGKETGRRL GMGTNMYPSS ALLGQDKDES
560 570 580 590 600
IASLPVDELI EKADGFAGVF PEHKYEIVKR LQEMKHICGM TGDGVNDAPA
610 620 630 640 650
LKRADIGIAV ADATDAARSA SDIVLTEPGL SVIVSAVLTS RAIFQRMKNY
660 670 680 690 700
TIYAVSITIR IVMGFMLLAL IWKFDFSPFM VLIVAILNDG TIMTISKDRV
710 720 730 740 750
KPSPLPDSWK LKEIFATGVV LGTYLAVMTV VFFWAAESTD FFSAKFGVRS
760 770 780 790 800
ISGNPHELTA AVYLQVSIVS QALIFVTRSR SWSYVERPGF WLISAFFMAQ
810 820 830 840 850
LIATLIAVYA NWNFARIRGI GWGWAGVIWL YSIVFYIPLD ILKFIIRYSL
860 870 880 890 900
SGRAWDNVIE NKTAFTSKKD YGKGEREAQW AQAQRTLHGL QPAQTSDMFN
910 920 930 940 950
DKSTYRELSE IADQAKRRAE VARLRERHTL KGHVESVVKQ KGLDIEAIQQ
HYTL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4HU00 | F4HU00_ARATH | Plasma membrane ATPase | HA9 AHA9, H(+)-ATPase 9, At1g80660, F23A5.1, F23A5_1 | 825 | Annotation score: | ||
A0A1P8AMR3 | A0A1P8AMR3_ARATH | Plasma membrane ATPase | HA9 AHA9, H(+)-ATPase 9, At1g80660, F23A5.1, F23A5_1 | 927 | Annotation score: | ||
A0A1P8AMR6 | A0A1P8AMR6_ARATH | Plasma membrane ATPase | HA9 AHA9, H(+)-ATPase 9, At1g80660, F23A5.1, F23A5_1 | 928 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73676 Genomic DNA No translation available. U53501 Genomic DNA Translation: AAA98916.1 Sequence problems. AC011713 Genomic DNA Translation: AAF14653.1 AC018849 Genomic DNA Translation: AAF27113.1 CP002684 Genomic DNA Translation: AEE36433.1 |
PIRi | H96838 S60301 |
RefSeqi | NP_178181.1, NM_106714.2 [Q42556-1] |
Genome annotation databases
EnsemblPlantsi | AT1G80660.1; AT1G80660.1; AT1G80660 [Q42556-1] |
GeneIDi | 844405 |
Gramenei | AT1G80660.1; AT1G80660.1; AT1G80660 [Q42556-1] |
KEGGi | ath:AT1G80660 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73676 Genomic DNA No translation available. U53501 Genomic DNA Translation: AAA98916.1 Sequence problems. AC011713 Genomic DNA Translation: AAF14653.1 AC018849 Genomic DNA Translation: AAF27113.1 CP002684 Genomic DNA Translation: AEE36433.1 |
PIRi | H96838 S60301 |
RefSeqi | NP_178181.1, NM_106714.2 [Q42556-1] |
3D structure databases
AlphaFoldDBi | Q42556 |
SMRi | Q42556 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 29623, 2 interactors |
STRINGi | 3702.AT1G80660.1 |
PTM databases
iPTMneti | Q42556 |
Proteomic databases
PaxDbi | Q42556 |
PRIDEi | Q42556 |
Genome annotation databases
EnsemblPlantsi | AT1G80660.1; AT1G80660.1; AT1G80660 [Q42556-1] |
GeneIDi | 844405 |
Gramenei | AT1G80660.1; AT1G80660.1; AT1G80660 [Q42556-1] |
KEGGi | ath:AT1G80660 |
Organism-specific databases
Araporti | AT1G80660 |
TAIRi | locus:2025727, AT1G80660 |
Phylogenomic databases
eggNOGi | KOG0205, Eukaryota |
InParanoidi | Q42556 |
PhylomeDBi | Q42556 |
Enzyme and pathway databases
BioCyci | ARA:AT1G80660-MONOMER |
Miscellaneous databases
PROi | PR:Q42556 |
Gene expression databases
ExpressionAtlasi | Q42556, baseline and differential |
Genevisiblei | Q42556, AT |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PMA9_ARATH | |
Accessioni | Q42556Primary (citable) accession number: Q42556 Secondary accession number(s): Q9M8N3, Q9SAJ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | January 11, 2001 | |
Last modified: | May 25, 2022 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families