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Protein

ATPase 9, plasma membrane-type

Gene

AHA9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3344-aspartylphosphate intermediateBy similarity1
Metal bindingi593MagnesiumBy similarity1
Metal bindingi597MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • proton-exporting ATPase activity, phosphorylative mechanism Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processHydrogen ion transport, Ion transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G80660-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase 9, plasma membrane-type (EC:3.6.3.6)
Alternative name(s):
Proton pump 9
Gene namesi
Name:AHA9
Ordered Locus Names:At1g80660
ORF Names:F23A5.1, T21F11.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G80660
TAIRilocus:2025727 AT1G80660

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 66CytoplasmicSequence analysisAdd BLAST66
Transmembranei67 – 86Helical; Name=1Sequence analysisAdd BLAST20
Topological domaini87 – 98ExtracellularSequence analysisAdd BLAST12
Transmembranei99 – 119Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini120 – 248CytoplasmicSequence analysisAdd BLAST129
Transmembranei249 – 269Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini270 – 278ExtracellularSequence analysis9
Transmembranei279 – 296Helical; Name=4Sequence analysisAdd BLAST18
Topological domaini297 – 648CytoplasmicSequence analysisAdd BLAST352
Transmembranei649 – 670Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini671 – 675ExtracellularSequence analysis5
Transmembranei676 – 698Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini699 – 714CytoplasmicSequence analysisAdd BLAST16
Transmembranei715 – 735Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini736 – 756ExtracellularSequence analysisAdd BLAST21
Transmembranei757 – 777Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini778 – 789CytoplasmicSequence analysisAdd BLAST12
Transmembranei790 – 810Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini811 – 818ExtracellularSequence analysis8
Transmembranei819 – 839Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini840 – 954CytoplasmicSequence analysisAdd BLAST115

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462821 – 954ATPase 9, plasma membrane-typeAdd BLAST954

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei886PhosphothreonineBy similarity1
Modified residuei936PhosphoserineBy similarity1
Modified residuei953PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ42556
PRIDEiQ42556

PTM databases

iPTMnetiQ42556

Expressioni

Tissue specificityi

Anther specific.

Gene expression databases

ExpressionAtlasiQ42556 differential
GenevisibleiQ42556 AT

Interactioni

Subunit structurei

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-953. Binding to 14-3-3 proteins activates the H+-ATPase (By similarity).By similarity

Protein-protein interaction databases

BioGridi29623, 1 interactor
STRINGi3702.AT1G80660.1

Structurei

3D structure databases

ProteinModelPortaliQ42556
SMRiQ42556
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni952 – 954Interaction with 14-3-3 proteinsBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205 Eukaryota
COG0474 LUCA
HOGENOMiHOG000160005
InParanoidiQ42556
KOiK01535
OMAiNFARIRG
OrthoDBiEOG093602AD
PhylomeDBiQ42556

Family and domain databases

CDDicd02076 P-type_ATPase_H, 1 hit
Gene3Di3.40.1110.10, 1 hit
InterProiView protein in InterPro
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR006534 P-type_ATPase_IIIA
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01647 ATPase-IIIA_H, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q42556-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGNKDSSWD DIKNEGIDLE KIPIEEVLTQ LRCTREGLTS DEGQTRLEIF
60 70 80 90 100
GPNKLEEKKE NKVLKFLGFM WNPLSWVMEL AAIMAIALAN GGGRPPDWQD
110 120 130 140 150
FVGITVLLII NSTISFIEEN NAGNAAAALM AGLAPKTKVL RDGKWSEQEA
160 170 180 190 200
AILVPGDIIS IKLGDIVPAD GRLLDGDPLK IDQSALTGES LPVTKHPGQE
210 220 230 240 250
VYSGSTCKQG ELEAVVIATG VHTFFGKAAH LVDSTNQEGH FQKVLTAIGN
260 270 280 290 300
FCICSIAIGM LIEIVVMYPI QKRAYRDGID NLLVLLIGGI PIAMPTVLSV
310 320 330 340 350
TMAIGSHRLS QQGAITKRMT AIEEMAGMDV LCSDKTGTLT LNKLTVDKSM
360 370 380 390 400
VEVFVKDLDK DQLLVNAARA SRVENQDAID ACIVGMLGDP REAREGITEV
410 420 430 440 450
HFFPFNPVDK RTAITYIDAN GNWHRVSKGA PEQIIELCNL REDASKRAHD
460 470 480 490 500
IIDKFADRGL RSLAVGRQTV SEKDKNSPGE PWQFLGLLPL FDPPRHDSAE
510 520 530 540 550
TIRRALDLGV NVKMITGDQL AIGKETGRRL GMGTNMYPSS ALLGQDKDES
560 570 580 590 600
IASLPVDELI EKADGFAGVF PEHKYEIVKR LQEMKHICGM TGDGVNDAPA
610 620 630 640 650
LKRADIGIAV ADATDAARSA SDIVLTEPGL SVIVSAVLTS RAIFQRMKNY
660 670 680 690 700
TIYAVSITIR IVMGFMLLAL IWKFDFSPFM VLIVAILNDG TIMTISKDRV
710 720 730 740 750
KPSPLPDSWK LKEIFATGVV LGTYLAVMTV VFFWAAESTD FFSAKFGVRS
760 770 780 790 800
ISGNPHELTA AVYLQVSIVS QALIFVTRSR SWSYVERPGF WLISAFFMAQ
810 820 830 840 850
LIATLIAVYA NWNFARIRGI GWGWAGVIWL YSIVFYIPLD ILKFIIRYSL
860 870 880 890 900
SGRAWDNVIE NKTAFTSKKD YGKGEREAQW AQAQRTLHGL QPAQTSDMFN
910 920 930 940 950
DKSTYRELSE IADQAKRRAE VARLRERHTL KGHVESVVKQ KGLDIEAIQQ

HYTL
Length:954
Mass (Da):105,208
Last modified:January 11, 2001 - v2
Checksum:i34DFCB96F45E5F18
GO

Sequence cautioni

The sequence AAA98916 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73676 Genomic DNA No translation available.
U53501 Genomic DNA Translation: AAA98916.1 Sequence problems.
AC011713 Genomic DNA Translation: AAF14653.1
AC018849 Genomic DNA Translation: AAF27113.1
CP002684 Genomic DNA Translation: AEE36433.1
PIRiH96838
S60301
RefSeqiNP_178181.1, NM_106714.2 [Q42556-1]

Genome annotation databases

EnsemblPlantsiAT1G80660.1; AT1G80660.1; AT1G80660 [Q42556-1]
GeneIDi844405
GrameneiAT1G80660.1; AT1G80660.1; AT1G80660 [Q42556-1]
KEGGiath:AT1G80660

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPMA9_ARATH
AccessioniPrimary (citable) accession number: Q42556
Secondary accession number(s): Q9M8N3, Q9SAJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: May 23, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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