UniProtKB - Q42523 (MCCA_ARATH)
Protein
Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
Gene
MCCA
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.By similarity
Miscellaneous
Temporal and spatial accumulation of the alpha and beta subunits during development at approximately equal molar ratios.
Catalytic activityi
- 3-methyl-(2E)-butenoyl-CoA + ATP + hydrogencarbonate = ADP + H+ + phosphate + trans-3-methylglutaconyl-CoAEC:6.4.1.4
Cofactori
Protein has several cofactor binding sites:: L-leucine degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Isovaleryl-CoA dehydrogenase, mitochondrial (IVD)
- Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCA), Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCB)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA, the pathway L-leucine degradation and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 152 | ATPBy similarity | 1 | |
Binding sitei | 236 | ATPBy similarity | 1 | |
Binding sitei | 271 | ATPBy similarity | 1 | |
Metal bindingi | 311 | Manganese 1By similarity | 1 | |
Metal bindingi | 325 | Manganese 1By similarity | 1 | |
Metal bindingi | 325 | Manganese 2By similarity | 1 | |
Metal bindingi | 327 | Manganese 2By similarity | 1 | |
Active sitei | 329 | By similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- CoA carboxylase activity Source: GO_Central
- cobalt ion binding Source: TAIR
- methylcrotonoyl-CoA carboxylase activity Source: TAIR
GO - Biological processi
- leucine catabolic process Source: TAIR
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Biotin, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT1G03090-MONOMER |
BRENDAi | 6.4.1.4, 399 |
UniPathwayi | UPA00363;UER00861 |
Names & Taxonomyi
Protein namesi | Recommended name: Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (EC:6.4.1.4)Short name: MCCase subunit alpha Alternative name(s): 3-methylcrotonyl-CoA carboxylase 1 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha |
Gene namesi | Name:MCCA Ordered Locus Names:At1g03090 ORF Names:F10O3.9, F10O3_8 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G03090 |
TAIRi | locus:2007559, AT1G03090 |
Subcellular locationi
Mitochondrion
Cytosol
- cytosolic ribosome Source: TAIR
Extracellular region or secreted
- extracellular region Source: TAIR
Mitochondrion
- mitochondrial matrix Source: UniProtKB-SubCell
- mitochondrion Source: TAIR
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 25 | MitochondrionSequence analysisAdd BLAST | 25 | |
ChainiPRO_0000002835 | 26 – 734 | Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrialAdd BLAST | 709 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 645 | PhosphoserineCombined sources | 1 | |
Modified residuei | 699 | N6-biotinyllysinePROSITE-ProRule annotationBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q42523 |
PRIDEi | Q42523 |
ProteomicsDBi | 238893 [Q42523-1] |
PTM databases
iPTMneti | Q42523 |
Expressioni
Tissue specificityi
In roots, cotyledons, leaves, flowers, ovaries, siliques and embryos.1 Publication
Gene expression databases
ExpressionAtlasi | Q42523, baseline and differential |
Genevisiblei | Q42523, AT |
Interactioni
Subunit structurei
Probably a heterodimer composed of biotin-containing alpha subunits and beta subunits.
By similarityProtein-protein interaction databases
BioGRIDi | 23601, 1 interactor |
STRINGi | 3702.AT1G03090.2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 37 – 484 | Biotin carboxylationAdd BLAST | 448 | |
Domaini | 156 – 354 | ATP-graspPROSITE-ProRule annotationAdd BLAST | 199 | |
Domaini | 657 – 733 | Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST | 77 |
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0238, Eukaryota |
InParanoidi | Q42523 |
OMAi | FEWENEY |
OrthoDBi | 254436at2759 |
PhylomeDBi | Q42523 |
Family and domain databases
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR005481, BC-like_N IPR001882, Biotin_BS IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR016185, PreATP-grasp_dom_sf IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif |
Pfami | View protein in Pfam PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit |
SMARTi | View protein in SMART SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00188, BIOTIN, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q42523-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSMMTVWALR RNVRRKNHSM LVRYISGSAS MKPKEQCIEK ILVANRGEIA
60 70 80 90 100
CRIMRTAKRL GIQTVAVYSD ADRDSLHVKS ADEAVRIGPP SARLSYLSGV
110 120 130 140 150
TIMEAAARTG AQAIHPGYGF LSESSDFAQL CEDSGLTFIG PPASAIRDMG
160 170 180 190 200
DKSASKRIMG AAGVPLVPGY HGHEQDIDHM KSEAEKIGYP IIIKPTHGGG
210 220 230 240 250
GKGMRIVQSG KDFADSFLGA QREAAASFGV NTILLEKYIT RPRHIEVQIF
260 270 280 290 300
GDKHGNVLHL YERDCSVQRR HQKIIEEAPA PNISEKFRAN LGQAAVSAAR
310 320 330 340 350
AVGYYNAGTV EFIVDTESDQ FYFMEMNTRL QVEHPVTEMI VGQDLVEWQI
360 370 380 390 400
RVANGEPLPL SQSEVPMSGH AFEARIYAEN VPKGFLPATG VLNHYRPVAV
410 420 430 440 450
SPSVRVETGV EQGDTVSMHY DPMIAKLVVW GGNRGEALVK LKDCLSNFQV
460 470 480 490 500
AGVPTNINFL QKLASHKEFA VGNVETHFIE HHKSDLFADE SNPAATEVAY
510 520 530 540 550
KAVKHSAALV AACISTIEHS TWNESNHGKV PSIWYSNPPF RVHHEAKQTI
560 570 580 590 600
ELEWNNECEG TGSNLISLGV RYQPDGSYLI EEGNDSPSLE LRVTRAGKCD
610 620 630 640 650
FRVEAAGLSM NVSLAAYLKD GYKHIHIWHG SEHHQFKQKV GIEFSEDEEG
660 670 680 690 700
VQHRTSSETS SHPPGTIVAP MAGLVVKVLV ENEAKVDQGQ PILVLEAMKM
710 720 730
EHVVKAPSSG SIQDLKVKAG QQVSDGSALF RIKG
Sequence cautioni
The sequence AAD25800 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 85 | V → D in AAA67356 (PubMed:7716229).Curated | 1 | |
Sequence conflicti | 92 | A → AK in AAA67356 (PubMed:7716229).Curated | 1 | |
Sequence conflicti | 430 | W → L in AAA67356 (PubMed:7716229).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008910 | 281 – 300 | Missing in isoform 2. 1 PublicationAdd BLAST | 20 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12536 mRNA Translation: AAA67356.1 AC006550 Genomic DNA Translation: AAD25800.1 Sequence problems. CP002684 Genomic DNA Translation: AEE27527.1 CP002684 Genomic DNA Translation: AEE27528.1 AY070723 mRNA Translation: AAL50065.1 |
PIRi | G86161 |
RefSeqi | NP_563674.1, NM_100191.4 [Q42523-2] NP_849583.1, NM_179252.3 [Q42523-1] |
Genome annotation databases
EnsemblPlantsi | AT1G03090.1; AT1G03090.1; AT1G03090 [Q42523-2] AT1G03090.2; AT1G03090.2; AT1G03090 [Q42523-1] |
GeneIDi | 838362 |
Gramenei | AT1G03090.1; AT1G03090.1; AT1G03090 [Q42523-2] AT1G03090.2; AT1G03090.2; AT1G03090 [Q42523-1] |
KEGGi | ath:AT1G03090 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12536 mRNA Translation: AAA67356.1 AC006550 Genomic DNA Translation: AAD25800.1 Sequence problems. CP002684 Genomic DNA Translation: AEE27527.1 CP002684 Genomic DNA Translation: AEE27528.1 AY070723 mRNA Translation: AAL50065.1 |
PIRi | G86161 |
RefSeqi | NP_563674.1, NM_100191.4 [Q42523-2] NP_849583.1, NM_179252.3 [Q42523-1] |
3D structure databases
SMRi | Q42523 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 23601, 1 interactor |
STRINGi | 3702.AT1G03090.2 |
PTM databases
iPTMneti | Q42523 |
Proteomic databases
PaxDbi | Q42523 |
PRIDEi | Q42523 |
ProteomicsDBi | 238893 [Q42523-1] |
Genome annotation databases
EnsemblPlantsi | AT1G03090.1; AT1G03090.1; AT1G03090 [Q42523-2] AT1G03090.2; AT1G03090.2; AT1G03090 [Q42523-1] |
GeneIDi | 838362 |
Gramenei | AT1G03090.1; AT1G03090.1; AT1G03090 [Q42523-2] AT1G03090.2; AT1G03090.2; AT1G03090 [Q42523-1] |
KEGGi | ath:AT1G03090 |
Organism-specific databases
Araporti | AT1G03090 |
TAIRi | locus:2007559, AT1G03090 |
Phylogenomic databases
eggNOGi | KOG0238, Eukaryota |
InParanoidi | Q42523 |
OMAi | FEWENEY |
OrthoDBi | 254436at2759 |
PhylomeDBi | Q42523 |
Enzyme and pathway databases
UniPathwayi | UPA00363;UER00861 |
BioCyci | ARA:AT1G03090-MONOMER |
BRENDAi | 6.4.1.4, 399 |
Miscellaneous databases
PROi | PR:Q42523 |
Gene expression databases
ExpressionAtlasi | Q42523, baseline and differential |
Genevisiblei | Q42523, AT |
Family and domain databases
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR005481, BC-like_N IPR001882, Biotin_BS IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR016185, PreATP-grasp_dom_sf IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif |
Pfami | View protein in Pfam PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit |
SMARTi | View protein in SMART SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00188, BIOTIN, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCCA_ARATH | |
Accessioni | Q42523Primary (citable) accession number: Q42523 Secondary accession number(s): Q9SA61 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2002 |
Last sequence update: | May 2, 2002 | |
Last modified: | April 7, 2021 | |
This is version 181 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways