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Protein

1-aminocyclopropane-1-carboxylate oxidase 2

Gene

ACO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Enzyme involved in the ethylene biosynthesis. Required to mediate the 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated reversion of the ABA-induced inhibition of seed germination via endosperm rupture. May promote stem elongation by maximizing the extensibility cells, possibly by activating ethylene biosynthesis, in response to very-long-chain fatty acids (VLCFAs C20:0 to C30:0).2 Publications

Catalytic activityi

1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O.

Cofactori

Fe2+PROSITE-ProRule annotation1 Publication, Cu2+PROSITE-ProRule annotation1 PublicationNote: Binds 1 Fe2+ ion per subunit. Can also bind Cu2+ ions.PROSITE-ProRule annotation1 Publication

Pathwayi: ethylene biosynthesis via S-adenosyl-L-methionine

This protein is involved in step 2 of the subpathway that synthesizes ethylene from S-adenosyl-L-methionine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1-aminocyclopropane-1-carboxylate synthase 4 (ACS4), 1-aminocyclopropane-1-carboxylate synthase 5 (ACS5), 1-aminocyclopropane-1-carboxylate synthase 7 (ACS7), 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8), 1-aminocyclopropane-1-carboxylate synthase 2 (ACS2), 1-aminocyclopropane-1-carboxylate synthase 11 (ACS11), 1-aminocyclopropane-1-carboxylate synthase 6 (ACS6), 1-aminocyclopropane-1-carboxylate synthase 9 (ACS9)
  2. 1-aminocyclopropane-1-carboxylate oxidase 2 (ACO2), 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4), 1-aminocyclopropane-1-carboxylate oxidase 5 (At1g77330), 1-aminocyclopropane-1-carboxylate oxidase 3 (At1g12010), 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1)
This subpathway is part of the pathway ethylene biosynthesis via S-adenosyl-L-methionine, which is itself part of Alkene biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ethylene from S-adenosyl-L-methionine, the pathway ethylene biosynthesis via S-adenosyl-L-methionine and in Alkene biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi180IronPROSITE-ProRule annotation1
Metal bindingi182IronPROSITE-ProRule annotation1
Metal bindingi237IronPROSITE-ProRule annotation1
Binding sitei2472-oxoglutaratePROSITE-ProRule annotation1

GO - Molecular functioni

  • 1-aminocyclopropane-1-carboxylate oxidase activity Source: TAIR
  • copper ion binding Source: TAIR
  • L-ascorbic acid binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to fatty acid Source: UniProtKB
  • cellular response to iron ion Source: TAIR
  • cellular response to nitric oxide Source: UniProtKB
  • defense response Source: UniProtKB-KW
  • detection of ethylene stimulus Source: TAIR
  • ethylene biosynthetic process Source: TAIR
  • positive regulation of seed germination Source: UniProtKB
  • response to cytokinin Source: TAIR
  • response to salt stress Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processEthylene biosynthesis, Plant defense
LigandCopper, Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

UniPathwayi
UPA00384;UER00563

Names & Taxonomyi

Protein namesi
Recommended name:
1-aminocyclopropane-1-carboxylate oxidase 2 (EC:1.14.17.4)
Short name:
ACC oxidase 2
Short name:
AtACO2
Gene namesi
Name:ACO2
Synonyms:EI305
Ordered Locus Names:At1g62380
ORF Names:F24O1.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G62380
TAIRilocus:2027099 AT1G62380

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Impaired in the 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated reversion of the ABA-induced inhibition of seed germination.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004082981 – 3201-aminocyclopropane-1-carboxylate oxidase 2Add BLAST320

Proteomic databases

PaxDbiQ41931
PRIDEiQ41931

PTM databases

iPTMnetiQ41931
SwissPalmiQ41931

Expressioni

Tissue specificityi

Expressed in vegetative tissues. Constitutively expressed in leaves and blades. In ethylene exposed etiolated seedlings, localized in cells at the outer side of the exaggerated hook in an ethylene-dependent manner and following an ethylene sensitive pattern. Also detected in the root tip when treated by ethylene.3 Publications

Inductioni

Upon iron deprivation. Induced by ethylene, particularly in root tips and hooks of ethiolated seedlings. Promoted by ozone O3. Accumulates in response to very-long-chain fatty acids (VLCFAs C20:0 to C30:0). Induced in roots by nitric oxide (NO).7 Publications

Gene expression databases

ExpressionAtlasiQ41931 baseline and differential
GenevisibleiQ41931 AT

Interactioni

Protein-protein interaction databases

BioGridi27757, 2 interactors
IntActiQ41931, 1 interactor
STRINGi3702.AT1G62380.1

Structurei

Secondary structure

1320
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ41931
SMRiQ41931
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 256Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST101

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili111 – 143Sequence analysisAdd BLAST33

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0143 Eukaryota
COG3491 LUCA
HOGENOMiHOG000276735
KOiK05933
OMAiALINEAC
OrthoDBiEOG09360IUD
PhylomeDBiQ41931

Family and domain databases

Gene3Di2.60.120.330, 1 hit
InterProiView protein in InterPro
IPR026992 DIOX_N
IPR027443 IPNS-like
IPR005123 Oxoglu/Fe-dep_dioxygenase
PfamiView protein in Pfam
PF03171 2OG-FeII_Oxy, 1 hit
PF14226 DIOX_N, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q41931-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKNMKFPVV DLSKLNGEER DQTMALINEA CENWGFFEIV NHGLPHDLMD
60 70 80 90 100
KIEKMTKDHY KTCQEQKFND MLKSKGLDNL ETEVEDVDWE STFYVRHLPQ
110 120 130 140 150
SNLNDISDVS DEYRTAMKDF GKRLENLAED LLDLLCENLG LEKGYLKKVF
160 170 180 190 200
HGTKGPTFGT KVSNYPPCPK PEMIKGLRAH TDAGGIILLF QDDKVSGLQL
210 220 230 240 250
LKDGDWIDVP PLNHSIVINL GDQLEVITNG KYKSVLHRVV TQQEGNRMSV
260 270 280 290 300
ASFYNPGSDA EISPATSLVE KDSEYPSFVF DDYMKLYAGV KFQPKEPRFA
310 320
AMKNASAVTE LNPTAAVETF
Length:320
Mass (Da):36,183
Last modified:November 1, 1998 - v2
Checksum:iE9B22DF21FDE35A4
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q94A28ACO2M_ARATH
Aconitate hydratase 2, mitochondria...
ACO2 At4g26970, F10M23.310
995Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti261E → D in AAC27484 (PubMed:10409511).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016100 mRNA Translation: AAC27484.1
AC003113 Genomic DNA Translation: AAF70838.1
CP002684 Genomic DNA Translation: AEE33960.1
AY045876 mRNA Translation: AAK76550.1
AY062685 mRNA Translation: AAL32763.1
AY093381 mRNA Translation: AAM13380.1
AY133851 mRNA Translation: AAM91785.1
AK230351 mRNA Translation: BAF02150.1
Z17775 mRNA Translation: CAA79062.1
PIRiT01448
T52267
RefSeqiNP_176428.1, NM_104918.5
UniGeneiAt.24621
At.37653

Genome annotation databases

EnsemblPlantsiAT1G62380.1; AT1G62380.1; AT1G62380
GeneIDi842536
GrameneiAT1G62380.1; AT1G62380.1; AT1G62380
KEGGiath:AT1G62380

Similar proteinsi

Entry informationi

Entry nameiACCO2_ARATH
AccessioniPrimary (citable) accession number: Q41931
Secondary accession number(s): O81093, Q7DLM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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