Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thiamine thiazole synthase 1, chloroplastic

Gene

THI1-1

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation1 Publication

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100Substrate; via amide nitrogenUniRule annotation1
Binding sitei128Substrate; via amide nitrogenUniRule annotation1
Binding sitei193Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei224SubstrateUniRule annotation1
Binding sitei239SubstrateUniRule annotation1
Binding sitei291Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthase 1, chloroplasticUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzyme 1UniRule annotation
Gene namesi
Name:THI1-1UniRule annotation
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Chromosome 8

Organism-specific databases

MaizeGDBi128724

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 45ChloroplastUniRule annotationAdd BLAST45
ChainiPRO_000003406246 – 354Thiamine thiazole synthase 1, chloroplasticAdd BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2222,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-222 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Proteomic databases

PRIDEiQ41738

Expressioni

Tissue specificityi

Highest expression in developing embryos and green leaves and a very low level expression seen in endosperm, roots, etiolated shoots and immature ears.

Developmental stagei

During embryo development, expression increases from 15-21 days after pollination and decreases slightly at day 24 and this level is maintained until day 36.

Gene expression databases

ExpressionAtlasiQ41738 differential
GenevisibleiQ41738 ZM

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ41738
SMRiQ41738
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 121Substrate bindingUniRule annotation2
Regioni301 – 303Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000106048
KOiK03146

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q41738-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAAASSLL KSSFAGSRLP AATRTTPASL VVATGPRGAG AGPICASMSM
60 70 80 90 100
SSSNPPYDLT SFRFSPIKES IVSREMTRRY MTDMITYADT DVVIVGAGSA
110 120 130 140 150
GLSCAYELSK DPAVSIAIVE QSVSPGGGAW LGGQLFSAMV VRKPAHLFLD
160 170 180 190 200
ELGVAYDEAE DYVVIKHAAL FTSTVMSLLL ARPNVKLFNA VAVEDLIVRG
210 220 230 240 250
GRVGGVVTNW ALVSMNHDTQ SCMDPNVMEA KVVVSSCGHD GPFGATGVKR
260 270 280 290 300
LQDIGMISAV PGMKALDMNT AEDEIVRLTR EVVPGMIVTG MEVAEIDGAP
310 320 330 340 350
RMGPTFGAMM ISGQKAAHLA LKALGRPNAV DGTMSPPLRE ELMIAYKDDE

VVDA
Length:354
Mass (Da):37,105
Last modified:November 1, 1996 - v1
Checksum:i1D27B449CB9294DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17350 mRNA Translation: AAA96738.1
PIRiS61419
RefSeqiNP_001105696.1, NM_001112226.1
UniGeneiZm.288

Genome annotation databases

EnsemblPlantsiZm00001d011183_T001; Zm00001d011183_P001; Zm00001d011183
GeneIDi542714
GrameneiZm00001d011183_T001; Zm00001d011183_P001; Zm00001d011183
KEGGizma:542714

Similar proteinsi

Entry informationi

Entry nameiTHI41_MAIZE
AccessioniPrimary (citable) accession number: Q41738
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health