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Protein

Flavonol synthase/flavanone 3-hydroxylase

Gene
N/A
Organism
Solanum tuberosum (Potato)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin.

Catalytic activityi

A dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O.By similarity
A (2S)-flavan-4-one + 2-oxoglutarate + O2 = a (2R,3R)-dihydroflavonol + succinate + CO2.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • L-ascorbateBy similarityNote: Binds 1 ascorbate molecule per subunit.By similarity

Pathwayi: flavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi238IronPROSITE-ProRule annotation1
Metal bindingi240IronPROSITE-ProRule annotation1
Metal bindingi291IronPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFlavonoid biosynthesis
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

UniPathwayiUPA00154

Names & Taxonomyi

Protein namesi
Recommended name:
Flavonol synthase/flavanone 3-hydroxylase (EC:1.14.11.9By similarity, EC:1.14.20.6By similarity)
Short name:
FLS
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
Proteomesi
  • UP000011115 Componenti: Unassembled WGS sequence

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000672971 – 349Flavonol synthase/flavanone 3-hydroxylaseAdd BLAST349

Expressioni

Developmental stagei

Temporally expressed during flower development.

Gene expression databases

ExpressionAtlasiQ41452 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400036565

Structurei

3D structure databases

ProteinModelPortaliQ41452
SMRiQ41452
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini213 – 310Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST98

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0143 Eukaryota
COG3491 LUCA

Family and domain databases

Gene3Di2.60.120.330, 1 hit
InterProiView protein in InterPro
IPR026992 DIOX_N
IPR027443 IPNS-like
IPR005123 Oxoglu/Fe-dep_dioxygenase
PfamiView protein in Pfam
PF03171 2OG-FeII_Oxy, 1 hit
PF14226 DIOX_N, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit

Sequencei

Sequence statusi: Complete.

Q41452-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTIQGQSAT TALTMEVARV QAISSITKCM DTIPSEYIRS ENEQPAATTL
60 70 80 90 100
QGVVLEVPVI DISNVDDDEE KLVKEIVEAS KEWGIFQVIN HGIPDEVIEN
110 120 130 140 150
LQKVGKEFFE EVPQEEKELI AKKPGAQSLE GYGTSLQKEI EGKKGWVDHL
160 170 180 190 200
FHKIWPPSAI NYRYWPKNPP SYREANEEYA KWLRKVADGI FRSLSLGLGL
210 220 230 240 250
EGHEMMEAAG SEDIVYMLKI NYYPPCPRPD LALGVVAHTD MSYITLLVPN
260 270 280 290 300
EVQVFKDGHW YDVNYIPNAI IVHIGDQVEI LSNGKYKSVY HRTTVNKYKT
310 320 330 340
RMSWPVFLEP SSEHEVGPIP NLINEANPPK FKTKKYKDYV YCKLNKLPQ
Length:349
Mass (Da):39,728
Last modified:November 1, 1997 - v1
Checksum:iADBBC3F6B10A0E05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92178 mRNA Translation: CAA63092.1
PIRiT07373
UniGeneiStu.200

Similar proteinsi

Entry informationi

Entry nameiFLS_SOLTU
AccessioniPrimary (citable) accession number: Q41452
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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