UniProtKB - Q3ZBF9 (PLPP_BOVIN)
Chronophin
PDXP
Functioni
Functions as a pyridoxal phosphate (PLP) phosphatase, which also catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), with order of substrate preference PLP > PNP > PMP and therefore plays a role in vitamin B6 metabolism (By similarity).
Also functions as a protein serine phosphatase that specifically dephosphorylates 'Ser-3' in proteins of the actin-depolymerizing factor (ADF)/cofilin family like CFL1 and DSTN. Thereby, regulates cofilin-dependent actin cytoskeleton reorganization, being required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phosphothreonines in LIMK1. Does not dephosphorylate peptides containing phosphotyrosine (PubMed:15580268).
By similarity1 PublicationCatalytic activityi
- EC:3.1.3.74By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:3.1.3.74By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:3.1.3.74By similarityThis reaction proceeds in the backwardBy similarity direction.
- EC:3.1.3.161 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 25 | NucleophileBy similarity | 1 | |
Metal bindingi | 25 | MagnesiumBy similarity | 1 | |
Active sitei | 27 | Proton donorBy similarity | 1 | |
Metal bindingi | 27 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 182 | SubstrateBy similarity | 1 | |
Binding sitei | 213 | SubstrateBy similarity | 1 | |
Metal bindingi | 238 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- magnesium ion binding Source: UniProtKB
- phosphoprotein phosphatase activity Source: UniProtKB
- protein serine/threonine phosphatase activity Source: UniProtKB-EC
- pyridoxal phosphatase activity Source: UniProtKB
GO - Biological processi
- positive regulation of actin filament depolymerization Source: UniProtKB
- protein dephosphorylation Source: UniProtKB
- pyridoxal phosphate catabolic process Source: UniProtKB
- regulation of cytokinesis Source: UniProtKB
- regulation of mitotic nuclear division Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Ligand | Magnesium, Metal-binding, Pyridoxal phosphate |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PDXPBy similarity Synonyms:CIN1 Publication |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- ruffle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
- lamellipodium membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Cytoskeleton
- cytoskeleton By similarity
Cytoplasm and Cytosol
- cytosol 1 Publication
Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Diffusely distributed throughout the cytosol during pro-metaphase and metaphase. Detected at the dynamic cell poles during telophase. Detected at the cleavage furrow and contractile ring during cytokinesis. Transiently detected at the plasma membrane in late stages of cytokinesis. Detected at the midbody.By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: UniProtKB
Plasma Membrane
- lamellipodium membrane Source: UniProtKB-SubCell
- ruffle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000254016 | 1 – 296 | ChronophinAdd BLAST | 296 |
Proteomic databases
PaxDbi | Q3ZBF9 |
PeptideAtlasi | Q3ZBF9 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000016505 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 58 – 60 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2882, Eukaryota |
InParanoidi | Q3ZBF9 |
OrthoDBi | 982374at2759 |
Family and domain databases
Gene3Di | 3.40.50.1000, 2 hits |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR006357, HAD-SF_hydro_IIA IPR023214, HAD_sf IPR006349, PGP_euk |
Pfami | View protein in Pfam PF13344, Hydrolase_6, 1 hit |
PIRSFi | PIRSF000915, PGP-type_phosphatase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01460, HAD-SF-IIA, 1 hit TIGR01452, PGP_euk, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MARCERLRGA ALRDVVGRAQ GVLFDCNGVL WNGERAVPGA PELLERLAQA
60 70 80 90 100
GKATLFVSNN SRRARPELAL RFARLGFGGL RSEQLFSSAL CAARLLRQRL
110 120 130 140 150
LGPPDTQGAV FVLGGEGLRA ELRAAGLRLA GDPSEDPGAA PRVRAVLVGY
160 170 180 190 200
DEHFSFAKLS EACAHLRDPD CLLVATDRDP WHPLSDGSRT PGTGSLAAAV
210 220 230 240 250
ETASGRQALV VGKPSPYMFE CITEHFSVDP GRTLMVGDRL ETDILFGHRC
260 270 280 290
GMTTVLTLTG VSSLEEAQAY LAAGQHDLVP HYYVESIADL MEGLED
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC103329 mRNA Translation: AAI03330.1 |
RefSeqi | NP_001030207.1, NM_001035035.2 |
Genome annotation databases
GeneIDi | 506308 |
KEGGi | bta:506308 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC103329 mRNA Translation: AAI03330.1 |
RefSeqi | NP_001030207.1, NM_001035035.2 |
3D structure databases
AlphaFoldDBi | Q3ZBF9 |
SMRi | Q3ZBF9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000016505 |
Proteomic databases
PaxDbi | Q3ZBF9 |
PeptideAtlasi | Q3ZBF9 |
Genome annotation databases
GeneIDi | 506308 |
KEGGi | bta:506308 |
Organism-specific databases
CTDi | 57026 |
Phylogenomic databases
eggNOGi | KOG2882, Eukaryota |
InParanoidi | Q3ZBF9 |
OrthoDBi | 982374at2759 |
Family and domain databases
Gene3Di | 3.40.50.1000, 2 hits |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR006357, HAD-SF_hydro_IIA IPR023214, HAD_sf IPR006349, PGP_euk |
Pfami | View protein in Pfam PF13344, Hydrolase_6, 1 hit |
PIRSFi | PIRSF000915, PGP-type_phosphatase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01460, HAD-SF-IIA, 1 hit TIGR01452, PGP_euk, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PLPP_BOVIN | |
Accessioni | Q3ZBF9Primary (citable) accession number: Q3ZBF9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | September 27, 2005 | |
Last modified: | May 25, 2022 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families