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Protein

Diguanylate cyclase DosC

Gene

dosC

Organism
Shigella sonnei (strain Ss046)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Globin-coupled heme-based oxygen sensor protein displaying diguanylate cyclase (DGC) activity in response to oxygen availability. Thus, catalyzes the synthesis of cyclic diguanylate (c-di-GMP) via the condensation of 2 GTP molecules. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria (By similarity).By similarity

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarityNote: Binds 1 heme group per subunit.By similarity
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei43Involved in oxygen binding and important for the stability of the Fe(II)-O(2) complexBy similarity1
Sitei60Important for oxygen binding and stability of the Fe(II)-O(2) complexBy similarity1
Sitei65Critical for restricting water access to the heme distal side to avoid rapid autoxidationBy similarity1
Metal bindingi98Iron (heme proximal ligand)By similarity1
Metal bindingi333MagnesiumBy similarity1
Sitei338Transition state stabilizerSequence analysis1
Binding sitei341SubstrateBy similarity1
Binding sitei350SubstrateBy similarity1
Active sitei376Proton acceptorSequence analysis1
Metal bindingi376MagnesiumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
LigandGTP-binding, Heme, Iron, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00599

Names & Taxonomyi

Protein namesi
Recommended name:
Diguanylate cyclase DosC (EC:2.7.7.65)
Short name:
DGC
Alternative name(s):
Direct oxygen-sensing cyclase
Gene namesi
Name:dosC
Ordered Locus Names:SSON_1634
OrganismiShigella sonnei (strain Ss046)
Taxonomic identifieri300269 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002529 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003161571 – 460Diguanylate cyclase DosCAdd BLAST460

Structurei

3D structure databases

ProteinModelPortaliQ3Z1N3
SMRiQ3Z1N3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini325 – 458GGDEFPROSITE-ProRule annotationAdd BLAST134

Domaini

Is composed of an N-terminal sensory globin-fold domain that binds heme and oxygen, and a C-terminal GGDEF diguanylate cyclase domain.By similarity

Phylogenomic databases

HOGENOMiHOG000006777
KOiK13069
OMAiDGHPDYE

Family and domain databases

CDDicd01949 GGDEF, 1 hit
Gene3Di1.10.490.10, 1 hit
InterProiView protein in InterPro
IPR000160 GGDEF_dom
IPR009050 Globin-like_sf
IPR012292 Globin/Proto
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00990 GGDEF, 1 hit
SMARTiView protein in SMART
SM00267 GGDEF, 1 hit
SUPFAMiSSF46458 SSF46458, 1 hit
SSF55073 SSF55073, 1 hit
TIGRFAMsiTIGR00254 GGDEF, 1 hit
PROSITEiView protein in PROSITE
PS50887 GGDEF, 1 hit

Sequencei

Sequence statusi: Complete.

Q3Z1N3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMYFKRMKD EWTGLVEQAD PLIRAKAAEI AVAHAHYLSI EFYRIVRIDP
60 70 80 90 100
HAEEFLSNEQ VERQLKSAME RWIINVLSAQ VDDVERLIQI QHTVAEVHAR
110 120 130 140 150
IGIPVEIVEM GFRVLKKILY PVIFSSDYSA AEKLQVYHFS INSIDIAMEV
160 170 180 190 200
MTRAFTFSDS SASKEDENYR IFSLLENAEE EKERQIASIL SWEIDIIYKI
210 220 230 240 250
LLDSDLGSSL PLSQADFGLW FNHKGRHYFS GIAEVGHISR LIQDFDGIFN
260 270 280 290 300
QTMRNTRNLN NRSLRVKFLL QIRNTVSQII TLLRELFEEV SRHEVGMDVL
310 320 330 340 350
TKLLNRRFLP TIFKREIAHA NRTGTPLSVL IIDVDKFKEI NDTWGHNTGD
360 370 380 390 400
EILRKVSQAF YDNVHSSDYV FRYGGDEFII VLTEASENET LRTAERIRSR
410 420 430 440 450
VEKTKLKAAN GEDIALSLSI GAAMFNGHPD YERLIQIADE ALYIAKRRGR
460
NRVELWKASL
Length:460
Mass (Da):53,175
Last modified:September 27, 2005 - v1
Checksum:i81C01550CAF5C4C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000038 Genomic DNA Translation: AAZ88329.1

Genome annotation databases

EnsemblBacteriaiAAZ88329; AAZ88329; SSON_1634
KEGGissn:SSON_1634

Similar proteinsi

Entry informationi

Entry nameiDOSC_SHISS
AccessioniPrimary (citable) accession number: Q3Z1N3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 27, 2005
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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