UniProtKB - Q3YLA6 (SRSF1_PIG)
Serine/arginine-rich splicing factor 1
SRSF1
Functioni
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway (By similarity).
By similarityGO - Molecular functioni
- RNA binding Source: UniProtKB
GO - Biological processi
- alternative mRNA splicing, via spliceosome Source: GO_Central
- mRNA 5'-splice site recognition Source: UniProtKB
- mRNA splicing, via spliceosome Source: GO_Central
- regulation of RNA splicing Source: UniProtKB
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
Reactomei | R-SSC-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-SSC-72163, mRNA Splicing - Major Pathway R-SSC-72165, mRNA Splicing - Minor Pathway R-SSC-72187, mRNA 3'-end processing R-SSC-73856, RNA Polymerase II Transcription Termination |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/arginine-rich splicing factor 1Alternative name(s): Splicing factor, arginine/serine-rich 1 |
Gene namesi | Name:SRSF1 Synonyms:SFRS1 |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:99084, SRSF1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Nucleus
- Nucleus speckle By similarity
Note: In nuclear speckles. Shuttles between the nucleus and the cytoplasm. Nuclear import is mediated via interaction with TNPO3.By similarity
Nucleus
- nuclear speck Source: GO_Central
- nucleoplasm Source: UniProtKB
- nucleus Source: UniProtKB
- spliceosomal complex Source: UniProtKB-KW
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000247977 | 2 – 248 | Serine/arginine-rich splicing factor 1Add BLAST | 247 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineSequence analysisBy similarity | 1 | |
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Cross-linki | 30 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 38 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 38 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 93 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 93 | Omega-N-methylarginine; alternateBy similarity | 1 | |
Modified residuei | 97 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 97 | Omega-N-methylarginine; alternateBy similarity | 1 | |
Modified residuei | 109 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 109 | Omega-N-methylarginine; alternateBy similarity | 1 | |
Modified residuei | 111 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 133 | PhosphoserineBy similarity | 1 | |
Modified residuei | 179 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 199 | PhosphoserineBy similarity | 1 | |
Modified residuei | 201 | PhosphoserineBy similarity | 1 | |
Modified residuei | 202 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 205 | PhosphoserineBy similarity | 1 | |
Modified residuei | 207 | PhosphoserineBy similarity | 1 | |
Modified residuei | 209 | PhosphoserineBy similarity | 1 | |
Modified residuei | 231 | PhosphoserineBy similarity | 1 | |
Modified residuei | 234 | PhosphoserineBy similarity | 1 | |
Modified residuei | 238 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q3YLA6 |
PeptideAtlasi | Q3YLA6 |
PRIDEi | Q3YLA6 |
Expressioni
Gene expression databases
Bgeei | ENSSSCG00000017626, Expressed in hindlimb bud and 48 other tissues |
ExpressionAtlasi | Q3YLA6, baseline |
Genevisiblei | Q3YLA6, SS |
Interactioni
Subunit structurei
Consists of two polypeptides of p32 and p33. In vitro, self-associates and binds SRSF2, SNRNP70 and U2AF1 but not U2AF2. Binds SREK1/SFRS12.
Interacts with SAFB/SAFB1.
Interacts with PSIP1/LEDGF.
Interacts with SRPK1. Identified in the spliceosome C complex.
Interacts with RSRC1 (via Arg/Ser-rich domain).
Interacts with ZRSR2/U2AF1-RS2.
Interacts with CCDC55 (via C-terminus).
Interacts with SRPK1 and a sliding docking interaction is essential for its sequential and processive phosphorylation by SRPK1.
Interacts with NXF1.
Interacts with CCNL1, CCNL2 and CDK11B.
Interacts with RRP1B.
Interacts (when phosphorylated in its RS domain) with TNPO3; promoting nuclear import.
Interacts with ILDR1 (via C-terminus) and ILDR2.
By similarityProtein-protein interaction databases
STRINGi | 9823.ENSSSCP00000018680 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 16 – 91 | RRM 1PROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 121 – 195 | RRM 2PROSITE-ProRule annotationAdd BLAST | 75 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 88 – 134 | DisorderedSequence analysisAdd BLAST | 47 | |
Regioni | 191 – 248 | DisorderedSequence analysisAdd BLAST | 58 | |
Regioni | 198 – 247 | Interaction with SAFB1By similarityAdd BLAST | 50 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 205 – 225 | Basic residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 234 – 248 | Basic residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0105, Eukaryota |
GeneTreei | ENSGT00940000155585 |
HOGENOMi | CLU_012062_34_0_1 |
InParanoidi | Q3YLA6 |
OMAi | HRMQISG |
OrthoDBi | 1321443at2759 |
TreeFami | TF106261 |
Family and domain databases
CDDi | cd12597, RRM1_SRSF1, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR034520, SRSF1_RRM1 |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR
60 70 80 90 100
RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPR SGRGTGRGGG
110 120 130 140 150
GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA
160 170 180 190 200
DVYRDGTGVV EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP
210 220 230 240
SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRT
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A287BMV2 | A0A287BMV2_PIG | Serine/arginine-rich splicing facto... | SRSF1 | 253 | Annotation score: | ||
A0A5S6GJG2 | A0A5S6GJG2_PIG | Serine/arginine-rich splicing facto... | SRSF1 | 236 | Annotation score: | ||
A0A5G2R2Z1 | A0A5G2R2Z1_PIG | Serine/arginine-rich splicing facto... | SRSF1 | 199 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ098951 Genomic DNA Translation: AAZ86089.1 DQ098950 mRNA Translation: AAZ86088.1 |
RefSeqi | NP_001033096.1, NM_001038007.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ098951 Genomic DNA Translation: AAZ86089.1 DQ098950 mRNA Translation: AAZ86088.1 |
RefSeqi | NP_001033096.1, NM_001038007.1 |
3D structure databases
AlphaFoldDBi | Q3YLA6 |
BMRBi | Q3YLA6 |
SMRi | Q3YLA6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000018680 |
Proteomic databases
PaxDbi | Q3YLA6 |
PeptideAtlasi | Q3YLA6 |
PRIDEi | Q3YLA6 |
Genome annotation databases
Organism-specific databases
CTDi | 6426 |
VGNCi | VGNC:99084, SRSF1 |
Phylogenomic databases
eggNOGi | KOG0105, Eukaryota |
GeneTreei | ENSGT00940000155585 |
HOGENOMi | CLU_012062_34_0_1 |
InParanoidi | Q3YLA6 |
OMAi | HRMQISG |
OrthoDBi | 1321443at2759 |
TreeFami | TF106261 |
Enzyme and pathway databases
Reactomei | R-SSC-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-SSC-72163, mRNA Splicing - Major Pathway R-SSC-72165, mRNA Splicing - Minor Pathway R-SSC-72187, mRNA 3'-end processing R-SSC-73856, RNA Polymerase II Transcription Termination |
Gene expression databases
Bgeei | ENSSSCG00000017626, Expressed in hindlimb bud and 48 other tissues |
ExpressionAtlasi | Q3YLA6, baseline |
Genevisiblei | Q3YLA6, SS |
Family and domain databases
CDDi | cd12597, RRM1_SRSF1, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR034520, SRSF1_RRM1 |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | SRSF1_PIG | |
Accessioni | Q3YLA6Primary (citable) accession number: Q3YLA6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 122 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families