UniProtKB - Q3V405 (Q3V405_MOUSE)
Protein
DNA damage-inducible transcript 3 protein
Gene
Ddit3
Organism
Mus musculus (Mouse)
Status
Functioni
Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.UniRule annotation
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription factor activity Source: InterPro
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cell cycle arrest Source: UniProtKB-KW
- response to unfolded protein Source: UniProtKB-KW
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | ActivatorUniRule annotation, DNA-bindingUniRule annotationARBA annotation, RepressorUniRule annotation |
Biological process | ApoptosisUniRule annotationARBA annotation, Cell cycle, Growth arrestUniRule annotation, Stress responseUniRule annotation, Transcription, Transcription regulationUniRule annotationARBA annotation, Unfolded protein responseUniRule annotation, Wnt signaling pathwayUniRule annotation |
Names & Taxonomyi
Protein namesi | Recommended name: DNA damage-inducible transcript 3 proteinUniRule annotationShort name: DDIT-3UniRule annotation Alternative name(s): C/EBP zetaUniRule annotation C/EBP-homologous proteinUniRule annotation C/EBP-homologous protein 10UniRule annotation CCAAT/enhancer-binding protein homologous proteinUniRule annotation Growth arrest and DNA-damage-inducible protein GADD153UniRule annotation |
Gene namesi | Name:Ddit3Imported |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Organism-specific databases
MGIi | MGI:109247, Ddit3 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmUniRule annotationARBA annotation, NucleusUniRule annotationARBA annotationPTM / Processingi
Post-translational modificationi
Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.UniRule annotation
Ubiquitinated, leading to its degradation by the proteasome.UniRule annotation
Keywords - PTMi
PhosphoproteinUniRule annotation, Ubl conjugationUniRule annotationARBA annotationInteractioni
Subunit structurei
Heterodimer.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 98 – 161 | BZIPInterPro annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 139 | DisorderedSequence analysisAdd BLAST | 106 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 34 – 58 | PolarSequence analysisAdd BLAST | 25 | |
Compositional biasi | 69 – 88 | PolarSequence analysisAdd BLAST | 20 | |
Compositional biasi | 115 – 139 | PolyampholyteSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Phylogenomic databases
OMAi | QCPARAG |
OrthoDBi | 1338770at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR016670, DNA_damage_induc_transcript_3 |
PANTHERi | PTHR16833, PTHR16833, 1 hit |
PIRSFi | PIRSF016571, C/EBPzeta_CHOP_DDIT3, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
i Sequence
Sequence statusi: Complete.
Q3V405-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAESLPFTL ETVSSWGLEA WYEDLQEVLS SDEIGGTYIS SPGNEEEESK
60 70 80 90 100
TFTTLDPASL AWLTEEPGPT EVTRTSQSPR SPDSSQSSMA QEEEEEEQGR
110 120 130 140 150
TRKRKQSGQC PARPGKQRMK EKEQENERKV AQLAEENERL KQEIERLTRE
160
VETTRRALID RMVSLHQA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028120 mRNA Translation: BAE20435.1 |
RefSeqi | NP_001277112.1, NM_001290183.1 NP_031863.3, NM_007837.4 |
Genome annotation databases
GeneIDi | 13198 |
KEGGi | mmu:13198 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028120 mRNA Translation: BAE20435.1 |
RefSeqi | NP_001277112.1, NM_001290183.1 NP_031863.3, NM_007837.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protocols and materials databases
DNASUi | 13198 |
Genome annotation databases
GeneIDi | 13198 |
KEGGi | mmu:13198 |
Organism-specific databases
CTDi | 1649 |
MGIi | MGI:109247, Ddit3 |
Phylogenomic databases
OMAi | QCPARAG |
OrthoDBi | 1338770at2759 |
Miscellaneous databases
BioGRID-ORCSi | 13198, 4 hits in 53 CRISPR screens |
ChiTaRSi | Ddit3, mouse |
SOURCEi | Search... |
Gene expression databases
Genevisiblei | Q3V405, MM |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR016670, DNA_damage_induc_transcript_3 |
PANTHERi | PTHR16833, PTHR16833, 1 hit |
PIRSFi | PIRSF016571, C/EBPzeta_CHOP_DDIT3, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q3V405_MOUSE | |
Accessioni | Q3V405Primary (citable) accession number: Q3V405 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | October 11, 2005 |
Last sequence update: | October 11, 2005 | |
Last modified: | April 7, 2021 | |
This is version 96 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |