UniProtKB - Q3V300 (KIF22_MOUSE)
Protein
Kinesin-like protein KIF22
Gene
Kif22
Organism
Mus musculus (Mouse)
Status
Functioni
Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA. Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 122 – 129 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- microtubule binding Source: GO_Central
- microtubule motor activity Source: GO_Central
GO - Biological processi
- DNA repair Source: InterPro
- metaphase plate congression Source: UniProtKB
- microtubule-based movement Source: GO_Central
- mitotic metaphase plate congression Source: UniProtKB
- sister chromatid cohesion Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, Motor protein |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2132295, MHC class II antigen presentation R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-MMU-983189, Kinesins |
Names & Taxonomyi
Protein namesi | Recommended name: Kinesin-like protein KIF22 |
Gene namesi | Name:Kif22 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109233, Kif22 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoskeleton
- cytoskeleton Curated
Cytoskeleton
- kinesin complex Source: GO_Central
- microtubule Source: GO_Central
- mitotic spindle Source: MGI
- spindle Source: MGI
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear speck Source: MGI
Other locations
- chromatin Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Microtubule, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000262921 | 1 – 660 | Kinesin-like protein KIF22Add BLAST | 660 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 407 | PhosphoserineBy similarity | 1 | |
Modified residuei | 422 | PhosphoserineBy similarity | 1 | |
Modified residuei | 447 | PhosphoserineBy similarity | 1 | |
Cross-linki | 460 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 540 | PhosphoserineBy similarity | 1 | |
Modified residuei | 576 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q3V300 |
MaxQBi | Q3V300 |
PaxDbi | Q3V300 |
PeptideAtlasi | Q3V300 |
PRIDEi | Q3V300 |
PTM databases
iPTMneti | Q3V300 |
PhosphoSitePlusi | Q3V300 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000030677, Expressed in thymus and 310 other tissues |
Genevisiblei | Q3V300, MM |
Interactioni
Subunit structurei
Interacts with FAM83D and SIAH1.
By similarityGO - Molecular functioni
- microtubule binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 225240, 5 interactors |
IntActi | Q3V300, 7 interactors |
STRINGi | 10090.ENSMUSP00000032915 |
Miscellaneous databases
RNActi | Q3V300, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 38 – 363 | Kinesin motorPROSITE-ProRule annotationAdd BLAST | 326 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 460 – 505 | Sequence analysisAdd BLAST | 46 |
Sequence similaritiesi
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0242, Eukaryota |
GeneTreei | ENSGT00940000159632 |
HOGENOMi | CLU_001485_27_1_1 |
InParanoidi | Q3V300 |
OMAi | NTGCLKE |
OrthoDBi | 787964at2759 |
PhylomeDBi | Q3V300 |
TreeFami | TF105233 |
Family and domain databases
Gene3Di | 3.40.850.10, 1 hit |
InterProi | View protein in InterPro IPR003583, Hlx-hairpin-Hlx_DNA-bd_motif IPR026986, KIF22 IPR027640, Kinesin-like_fam IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase IPR010994, RuvA_2-like |
PANTHERi | PTHR24115, PTHR24115, 1 hit PTHR24115:SF801, PTHR24115:SF801, 1 hit |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM00278, HhH1, 2 hits SM00129, KISc, 1 hit |
SUPFAMi | SSF47781, SSF47781, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q3V300-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLRAKTCPQ RREMASATSG PGRCVSKGGL GRRPPLARVR VAVRLRPFMD
60 70 80 90 100
GETEAKELPC VRAIDSCSLE VANWKKYQET LKYQFDAFYG EKSTQQEVYV
110 120 130 140 150
GSVQPILRHL LEGQNASVLA YGPTGAGKTH TMLGSPEQPG VIPRALMDLL
160 170 180 190 200
QLAREESAEG RPWDVSVAMS YLEIYQEKVL DLLDPASGDL VIREDCRGNI
210 220 230 240 250
LIPGLTQKPI TSFSDFEQHF LPASRNRAVG ATRLNQRSSR SHAVLLVKVD
260 270 280 290 300
QRERLTPFRQ REGKLYLIDL AGSEDNRRTG NQGIRLKESG AINTSLFVLG
310 320 330 340 350
KVVDALNQGL PRIPYRDSKL TRLLQDSLGG SAHSILIANI APERRFYQDT
360 370 380 390 400
ISALNFTARS KEVINRPFTN ESLQPHALAP VKLSQKELLG PSEAKKAKGP
410 420 430 440 450
EEESTGSPES TAAPASASQK LSLLQKLSNM DPAMLENLLS MERLLGSQGS
460 470 480 490 500
QGTPLLNTPK RERMVLMKTV EEKNLEIERL KMKQKELEAK VLAQEAPDPR
510 520 530 540 550
EKENTPTILQ PPASYSGTVA KPLKKAVVMP LQRIQKQRES SNQIQLLKKG
560 570 580 590 600
PKRKLEPSPE SEAVEKDEDY WEVQISPELL AHGRKKLLDL LNEGSARELR
610 620 630 640 650
SLQRIGQKKA QLIVGWRELH GPFSEVEDLE QVEGISGKQV ESFLKANLLS
660
LAASQHSGPS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0U1RNY7 | A0A0U1RNY7_MOUSE | Transcription elongation factor, mi... | Kif22 | 151 | Annotation score: | ||
A0A0U1RPH8 | A0A0U1RPH8_MOUSE | Kinesin-like protein KIF22 | Kif22 | 161 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 60 | C → R in BAE43356 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 267 | L → I in BAE43356 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 528 | V → E in BAE43356 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK075922 mRNA Translation: BAE43356.1 BC003427 mRNA Translation: AAH03427.1 AF013119 mRNA Translation: AAC39968.1 |
CCDSi | CCDS21855.1 |
RefSeqi | NP_663563.1, NM_145588.1 |
Genome annotation databases
Ensembli | ENSMUST00000032915; ENSMUSP00000032915; ENSMUSG00000030677 |
GeneIDi | 110033 |
KEGGi | mmu:110033 |
UCSCi | uc009jud.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK075922 mRNA Translation: BAE43356.1 BC003427 mRNA Translation: AAH03427.1 AF013119 mRNA Translation: AAC39968.1 |
CCDSi | CCDS21855.1 |
RefSeqi | NP_663563.1, NM_145588.1 |
3D structure databases
SMRi | Q3V300 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 225240, 5 interactors |
IntActi | Q3V300, 7 interactors |
STRINGi | 10090.ENSMUSP00000032915 |
PTM databases
iPTMneti | Q3V300 |
PhosphoSitePlusi | Q3V300 |
Proteomic databases
EPDi | Q3V300 |
MaxQBi | Q3V300 |
PaxDbi | Q3V300 |
PeptideAtlasi | Q3V300 |
PRIDEi | Q3V300 |
Protocols and materials databases
Antibodypediai | 13324, 238 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000032915; ENSMUSP00000032915; ENSMUSG00000030677 |
GeneIDi | 110033 |
KEGGi | mmu:110033 |
UCSCi | uc009jud.1, mouse |
Organism-specific databases
CTDi | 3835 |
MGIi | MGI:109233, Kif22 |
Phylogenomic databases
eggNOGi | KOG0242, Eukaryota |
GeneTreei | ENSGT00940000159632 |
HOGENOMi | CLU_001485_27_1_1 |
InParanoidi | Q3V300 |
OMAi | NTGCLKE |
OrthoDBi | 787964at2759 |
PhylomeDBi | Q3V300 |
TreeFami | TF105233 |
Enzyme and pathway databases
Reactomei | R-MMU-2132295, MHC class II antigen presentation R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-MMU-983189, Kinesins |
Miscellaneous databases
BioGRID-ORCSi | 110033, 2 hits in 17 CRISPR screens |
ChiTaRSi | Kif22, mouse |
PROi | PR:Q3V300 |
RNActi | Q3V300, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030677, Expressed in thymus and 310 other tissues |
Genevisiblei | Q3V300, MM |
Family and domain databases
Gene3Di | 3.40.850.10, 1 hit |
InterProi | View protein in InterPro IPR003583, Hlx-hairpin-Hlx_DNA-bd_motif IPR026986, KIF22 IPR027640, Kinesin-like_fam IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase IPR010994, RuvA_2-like |
PANTHERi | PTHR24115, PTHR24115, 1 hit PTHR24115:SF801, PTHR24115:SF801, 1 hit |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM00278, HhH1, 2 hits SM00129, KISc, 1 hit |
SUPFAMi | SSF47781, SSF47781, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KIF22_MOUSE | |
Accessioni | Q3V300Primary (citable) accession number: Q3V300 Secondary accession number(s): O35232, Q99LC7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | November 28, 2006 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families