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Entry version 128 (12 Aug 2020)
Sequence version 1 (11 Oct 2005)
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Protein

DNA-directed RNA polymerase II subunit E

Gene

Polr2e

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process.ARBA annotation

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processTranscriptionARBA annotation

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit EARBA annotation
Alternative name(s):
RPB5 homologARBA annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Polr2eImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:1913670, Polr2e

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

NucleusARBA annotation

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Proteomic databases

Encyclopedia of Proteome Dynamics

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EPDi
Q3V214

PeptideAtlas

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PeptideAtlasi
Q3V214

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000004667, Expressed in embryo and 309 other tissues

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q3V214

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini4 – 94RNA_pol_Rpb5_NInterPro annotationAdd BLAST91
Domaini137 – 209RNA_pol_Rpb5_CInterPro annotationAdd BLAST73

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.ARBA annotation

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
CLU_058320_0_1_1

KEGG Orthology (KO)

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KOi
K03013

Identification of Orthologs from Complete Genome Data

More...
OMAi
MQMCHDR

Database of Orthologous Groups

More...
OrthoDBi
1255823at2759

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
3.40.1340.10, 1 hit
3.90.940.20, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00025, RNApol_RpoH_RPB5, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR014381, DNA_RNA_pol_RPB5_euk/virus
IPR005571, RNA_pol_Rpb5_N
IPR036710, RNA_pol_Rpb5_N_sf
IPR000783, RNA_pol_subH/Rpb5_C
IPR020608, RNA_pol_subH/Rpb5_CS
IPR039531, Rpb5-like
IPR035913, RPB5-like_sf
IPR020609, RpoH/RPB5

The PANTHER Classification System

More...
PANTHERi
PTHR10535, PTHR10535, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01191, RNA_pol_Rpb5_C, 1 hit
PF03871, RNA_pol_Rpb5_N, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF000747, RPB5, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53036, SSF53036, 1 hit
SSF55287, SSF55287, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS01110, RNA_POL_H_23KD, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q3V214-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE
60 70 80 90 100
GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT
110 120 130 140 150
RALIVVQQGM TPSAKQSLVD MAPKYVLEQF LQQELLINIT EHELVPEHVV
160 170 180 190 200
MTKEEVTELL ARYKLRESQL PRIQAGDPVA RYFGIKRGQV VKIIRPSETA
210
GRYITYRLVQ
Length:210
Mass (Da):24,570
Last modified:October 11, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE09DEB40E05F3554
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AK132109 mRNA Translation: BAE20985.1
AK144668 mRNA Translation: BAE25998.1
AK145201 mRNA Translation: BAE26292.1
AK015823 mRNA Translation: BAE43245.1

NCBI Reference Sequences

More...
RefSeqi
NP_079830.2, NM_025554.2

Genome annotation databases

Database of genes from NCBI RefSeq genomes

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GeneIDi
66420

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:66420

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK132109 mRNA Translation: BAE20985.1
AK144668 mRNA Translation: BAE25998.1
AK145201 mRNA Translation: BAE26292.1
AK015823 mRNA Translation: BAE43245.1
RefSeqiNP_079830.2, NM_025554.2

3D structure databases

SMRiQ3V214
ModBaseiSearch...

Proteomic databases

EPDiQ3V214
PeptideAtlasiQ3V214

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...
Antibodypediai
22550, 295 antibodies

The DNASU plasmid repository

More...
DNASUi
66420

Genome annotation databases

GeneIDi66420
KEGGimmu:66420

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
5434
MGIiMGI:1913670, Polr2e

Phylogenomic databases

HOGENOMiCLU_058320_0_1_1
KOiK03013
OMAiMQMCHDR
OrthoDBi1255823at2759

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
66420, 8 hits in 18 CRISPR screens

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000004667, Expressed in embryo and 309 other tissues

Family and domain databases

Gene3Di3.40.1340.10, 1 hit
3.90.940.20, 1 hit
HAMAPiMF_00025, RNApol_RpoH_RPB5, 1 hit
InterProiView protein in InterPro
IPR014381, DNA_RNA_pol_RPB5_euk/virus
IPR005571, RNA_pol_Rpb5_N
IPR036710, RNA_pol_Rpb5_N_sf
IPR000783, RNA_pol_subH/Rpb5_C
IPR020608, RNA_pol_subH/Rpb5_CS
IPR039531, Rpb5-like
IPR035913, RPB5-like_sf
IPR020609, RpoH/RPB5
PANTHERiPTHR10535, PTHR10535, 1 hit
PfamiView protein in Pfam
PF01191, RNA_pol_Rpb5_C, 1 hit
PF03871, RNA_pol_Rpb5_N, 1 hit
PIRSFiPIRSF000747, RPB5, 1 hit
SUPFAMiSSF53036, SSF53036, 1 hit
SSF55287, SSF55287, 1 hit
PROSITEiView protein in PROSITE
PS01110, RNA_POL_H_23KD, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiQ3V214_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q3V214
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: August 12, 2020
This is version 128 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)
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