UniProtKB - Q3V1T4 (P3H1_MOUSE)
Protein
Prolyl 3-hydroxylase 1
Gene
P3h1
Organism
Mus musculus (Mouse)
Status
Functioni
Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts (By similarity).By similarity
Catalytic activityi
- 2-oxoglutarate + L-prolyl-[procollagen] + O2 = CO2 + succinate + trans-3-hydroxy-L-prolyl-[procollagen]EC:1.14.11.7
Cofactori
Protein has several cofactor binding sites:- Fe cationBy similarity
- L-ascorbateBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 590 | Iron | 1 | |
Metal bindingi | 592 | Iron | 1 | |
Metal bindingi | 662 | Iron | 1 | |
Active sitei | 672 | By similarity | 1 |
GO - Molecular functioni
- iron ion binding Source: InterPro
- L-ascorbic acid binding Source: UniProtKB-KW
- procollagen-proline 3-dioxygenase activity Source: MGI
GO - Biological processi
- bone development Source: MGI
- collagen fibril organization Source: MGI
- collagen metabolic process Source: MGI
- negative regulation of cell growth Source: MGI
- negative regulation of post-translational protein modification Source: MGI
- positive regulation of neuron projection development Source: MGI
- protein folding Source: MGI
- protein hydroxylation Source: MGI
- protein stabilization Source: MGI
- regulation of ossification Source: MGI
- regulation of protein secretion Source: MGI
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Ligand | Iron, Metal-binding, Vitamin C |
Enzyme and pathway databases
BRENDAi | 1.14.11.7, 3474 |
Reactomei | R-MMU-1650814, Collagen biosynthesis and modifying enzymes |
Names & Taxonomyi
Protein namesi | Recommended name: Prolyl 3-hydroxylase 1Imported (EC:1.14.11.7)Alternative name(s): Growth suppressor 11 Publication Leucine- and proline-enriched proteoglycan 1By similarity Short name: Leprecan-1By similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1888921, P3h1 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
Endoplasmic reticulum
- Endoplasmic reticulum PROSITE-ProRule annotation
Note: Secreted into the extracellular matrix as a chondroitin sulfate proteoglycan (CSPG).
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Extracellular region or secreted
- basement membrane Source: MGI
Nucleus
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Endoplasmic reticulum, Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000240353 | 26 – 739 | Prolyl 3-hydroxylase 1Add BLAST | 714 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 319 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 470 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 543 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
O-glycosylated; chondroitin sulfate.By similarity
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q3V1T4 |
PeptideAtlasi | Q3V1T4 |
PRIDEi | Q3V1T4 |
PTM databases
GlyGeni | Q3V1T4, 3 sites |
iPTMneti | Q3V1T4 |
PhosphoSitePlusi | Q3V1T4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028641, Expressed in humerus cartilage element and 257 other tissues |
ExpressionAtlasi | Q3V1T4, baseline and differential |
Genevisiblei | Q3V1T4, MM |
Interactioni
Protein-protein interaction databases
IntActi | Q3V1T4, 1 interactor |
MINTi | Q3V1T4 |
STRINGi | 10090.ENSMUSP00000099723 |
Miscellaneous databases
RNActi | Q3V1T4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 36 – 69 | TPR 1Add BLAST | 34 | |
Repeati | 146 – 179 | TPR 2Add BLAST | 34 | |
Repeati | 208 – 241 | TPR 3Add BLAST | 34 | |
Repeati | 304 – 337 | TPR 4Add BLAST | 34 | |
Domaini | 567 – 681 | Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST | 115 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 404 – 442 | Sequence analysisAdd BLAST | 39 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 736 – 739 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Sequence similaritiesi
Belongs to the leprecan family.Curated
Keywords - Domaini
Coiled coil, Repeat, Signal, TPR repeatPhylogenomic databases
eggNOGi | KOG4459, Eukaryota |
GeneTreei | ENSGT00940000158725 |
HOGENOMi | CLU_017820_1_0_1 |
InParanoidi | Q3V1T4 |
OMAi | MFQGVTV |
OrthoDBi | 660619at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR005123, Oxoglu/Fe-dep_dioxygenase IPR039575, P3H IPR039837, P3H1 IPR006620, Pro_4_hyd_alph IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR14049, PTHR14049, 1 hit PTHR14049:SF5, PTHR14049:SF5, 1 hit |
Pfami | View protein in Pfam PF13640, 2OG-FeII_Oxy_3, 1 hit |
SMARTi | View protein in SMART SM00702, P4Hc, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit PS51471, FE2OG_OXY, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q3V1T4-1) [UniParc]FASTAAdd to basket
Also known as: GROS1-L
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAVSERRLLA AMLAVAAAAA LRVAAESEPG WDVAAPDLLY AEGTAAYSRG
60 70 80 90 100
DWPGVVLNME RALRSRAALR ALRLRCRTRC ATELPWAPDL DLGPDPSLSQ
110 120 130 140 150
DPGAAALHDL RFFGAVLRRA ACLRRCLGPP SAHLLSEELD LEFNKRSPYN
160 170 180 190 200
YLQVAYFKIN KLEKAVAAAH TFFVGNPEHM EMRQNLDYYQ TMSGVKEADF
210 220 230 240 250
RDLEAKPHMH EFRLGVRLYS EEKPQEAVPH LEAALQEYFV ADEECRALCE
260 270 280 290 300
GPYDYDGYNY LDYSADLFQA ITDHYVQVLN CKQNCVTELA SHPSREKPFE
310 320 330 340 350
DFLPSHYNYL QFAYYNIGNY TQAIECAKTY LLFFPNDEVM HQNLAYYTAM
360 370 380 390 400
LGEEEASSIS PRENAEEYRR RSLLEKELLF FAYDIFGIPF VDPDSWTPEE
410 420 430 440 450
VIPKRLQEKQ KSERETAVRI SQEIGNLMKE IETLVEEKTK ESLDVSRLTR
460 470 480 490 500
EGGPLLYEGI SLTMNSKVLN GSQRVVMDGV ISDDECQELQ RLTNAAATSG
510 520 530 540 550
DGYRGQTSPH TPNEKFYGVT VLKALKLGQE GKVPLQSARM YYNVTEKVRR
560 570 580 590 600
VMESYFRLDT PLYFSYSHLV CRTAIEESQA ERKDSSHPVH VDNCILNAEA
610 620 630 640 650
LMCIKEPPAY TFRDYSAILY LNGDFDGGNF YFTELDAKTV TAEVQPQCGR
660 670 680 690 700
AVGFSSGTEN PHGVKAVTRG QRCAIALWFT LDPRHSERDR VQADDLVKML
710 720 730
FSPEEVDLPQ EQPLPDQQGS PEPGEESLSD RGSLHKDEL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2A7Q5 | A2A7Q5_MOUSE | Procollagen-proline 3-dioxygenase | P3h1 | 748 | Annotation score: | ||
A6PW84 | A6PW84_MOUSE | Procollagen-proline 3-dioxygenase | P3h1 | 746 | Annotation score: | ||
F2Z499 | F2Z499_MOUSE | Prolyl 3-hydroxylase 1 | P3h1 | 363 | Annotation score: |
Sequence cautioni
The sequence BAB27041 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 – 25 | SERRL…LRVAA → TKGGCWHDASGRRRRRLTGC G in AAF04806 (PubMed:10951563).CuratedAdd BLAST | 22 | |
Sequence conflicti | 4 – 25 | SERRL…LRVAA → TKGGCWHDASGRRRRRLTGC G in AAF04807 (PubMed:10951563).CuratedAdd BLAST | 22 | |
Sequence conflicti | 50 | G → R in AAF04806 (PubMed:10951563).Curated | 1 | |
Sequence conflicti | 50 | G → R in AAF04807 (PubMed:10951563).Curated | 1 | |
Sequence conflicti | 371 – 372 | RS → PN in AAF04806 (PubMed:10951563).Curated | 2 | |
Sequence conflicti | 371 – 372 | RS → PN in AAF04807 (PubMed:10951563).Curated | 2 | |
Sequence conflicti | 403 | P → T in BAE21065 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 420 | Missing in BAC26962 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 484 | D → N in BAE35138 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 569 | L → F in AAF04806 (PubMed:10951563).Curated | 1 | |
Sequence conflicti | 589 | V → D in BAE21065 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 601 | L → F in AAF04806 (PubMed:10951563).Curated | 1 | |
Sequence conflicti | 614 | D → E in AAF04806 (PubMed:10951563).Curated | 1 | |
Sequence conflicti | 685 | H → Q in BAC26962 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 716 | D → G in BAE35138 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 716 | D → G in AAH24047 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 727 – 739 | SLSDR…HKDEL → FLHGATVLGVGIA in AAF04806 (PubMed:10951563).CuratedAdd BLAST | 13 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019349 | 1 – 179 | Missing in isoform 3. 1 PublicationAdd BLAST | 179 | |
Alternative sequenceiVSP_019350 | 540 – 543 | MYYN → TALQ in isoform 2. 1 Publication | 4 | |
Alternative sequenceiVSP_019351 | 544 – 739 | Missing in isoform 2. 1 PublicationAdd BLAST | 196 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF165163 mRNA Translation: AAF04806.1 Sequence problems. AF165164 mRNA Translation: AAF04807.1 Sequence problems. AK010578 mRNA Translation: BAB27041.1 Frameshift. AK030436 mRNA Translation: BAC26962.1 AK132262 mRNA Translation: BAE21065.1 Sequence problems. AK159505 mRNA Translation: BAE35138.1 AL606975 Genomic DNA No translation available. BC024047 mRNA Translation: AAH24047.1 |
CCDSi | CCDS38859.1 [Q3V1T4-1] CCDS38860.1 [Q3V1T4-3] |
RefSeqi | NP_001035874.1, NM_001042411.1 [Q3V1T4-3] NP_001273077.1, NM_001286148.1 NP_062756.2, NM_019782.3 NP_062757.2, NM_019783.2 [Q3V1T4-1] |
Genome annotation databases
Ensembli | ENSMUST00000081606; ENSMUSP00000080312; ENSMUSG00000028641 [Q3V1T4-3] ENSMUST00000121111; ENSMUSP00000112504; ENSMUSG00000028641 [Q3V1T4-1] |
GeneIDi | 56401 |
KEGGi | mmu:56401 |
UCSCi | uc008ulq.1, mouse [Q3V1T4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF165163 mRNA Translation: AAF04806.1 Sequence problems. AF165164 mRNA Translation: AAF04807.1 Sequence problems. AK010578 mRNA Translation: BAB27041.1 Frameshift. AK030436 mRNA Translation: BAC26962.1 AK132262 mRNA Translation: BAE21065.1 Sequence problems. AK159505 mRNA Translation: BAE35138.1 AL606975 Genomic DNA No translation available. BC024047 mRNA Translation: AAH24047.1 |
CCDSi | CCDS38859.1 [Q3V1T4-1] CCDS38860.1 [Q3V1T4-3] |
RefSeqi | NP_001035874.1, NM_001042411.1 [Q3V1T4-3] NP_001273077.1, NM_001286148.1 NP_062756.2, NM_019782.3 NP_062757.2, NM_019783.2 [Q3V1T4-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q3V1T4, 1 interactor |
MINTi | Q3V1T4 |
STRINGi | 10090.ENSMUSP00000099723 |
PTM databases
GlyGeni | Q3V1T4, 3 sites |
iPTMneti | Q3V1T4 |
PhosphoSitePlusi | Q3V1T4 |
Proteomic databases
PaxDbi | Q3V1T4 |
PeptideAtlasi | Q3V1T4 |
PRIDEi | Q3V1T4 |
Protocols and materials databases
Antibodypediai | 32239, 284 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000081606; ENSMUSP00000080312; ENSMUSG00000028641 [Q3V1T4-3] ENSMUST00000121111; ENSMUSP00000112504; ENSMUSG00000028641 [Q3V1T4-1] |
GeneIDi | 56401 |
KEGGi | mmu:56401 |
UCSCi | uc008ulq.1, mouse [Q3V1T4-1] |
Organism-specific databases
CTDi | 64175 |
MGIi | MGI:1888921, P3h1 |
Phylogenomic databases
eggNOGi | KOG4459, Eukaryota |
GeneTreei | ENSGT00940000158725 |
HOGENOMi | CLU_017820_1_0_1 |
InParanoidi | Q3V1T4 |
OMAi | MFQGVTV |
OrthoDBi | 660619at2759 |
Enzyme and pathway databases
BRENDAi | 1.14.11.7, 3474 |
Reactomei | R-MMU-1650814, Collagen biosynthesis and modifying enzymes |
Miscellaneous databases
BioGRID-ORCSi | 56401, 0 hits in 17 CRISPR screens |
ChiTaRSi | P3h1, mouse |
PROi | PR:Q3V1T4 |
RNActi | Q3V1T4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028641, Expressed in humerus cartilage element and 257 other tissues |
ExpressionAtlasi | Q3V1T4, baseline and differential |
Genevisiblei | Q3V1T4, MM |
Family and domain databases
InterProi | View protein in InterPro IPR005123, Oxoglu/Fe-dep_dioxygenase IPR039575, P3H IPR039837, P3H1 IPR006620, Pro_4_hyd_alph IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR14049, PTHR14049, 1 hit PTHR14049:SF5, PTHR14049:SF5, 1 hit |
Pfami | View protein in Pfam PF13640, 2OG-FeII_Oxy_3, 1 hit |
SMARTi | View protein in SMART SM00702, P4Hc, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit PS51471, FE2OG_OXY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | P3H1_MOUSE | |
Accessioni | Q3V1T4Primary (citable) accession number: Q3V1T4 Secondary accession number(s): A2A7Q4 Q9QZT7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | June 27, 2006 | |
Last modified: | December 2, 2020 | |
This is version 129 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families