UniProtKB - Q3V0C5 (UBP48_MOUSE)
Ubiquitin carboxyl-terminal hydrolase 48
Usp48
Functioni
Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites (By similarity).
By similarityCatalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 98 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 353 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: GO_Central
- thiol-dependent deubiquitinase Source: GO_Central
GO - Biological processi
- protein deubiquitination Source: GO_Central
- ubiquitin-dependent protein catabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-5689880, Ub-specific processing proteases |
Protein family/group databases
MEROPSi | C19.068 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin carboxyl-terminal hydrolase 48 (EC:3.4.19.12)Alternative name(s): Deubiquitinating enzyme 48 Ubiquitin thioesterase 48 Ubiquitin-specific-processing protease 48 |
Gene namesi | Name:Usp48 Synonyms:Kiaa4202 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2158502, Usp48 |
VEuPathDBi | HostDB:ENSMUSG00000043411 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Plasma Membrane
- postsynaptic membrane Source: MGI
Other locations
- membrane raft Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249524 | 1 – 1052 | Ubiquitin carboxyl-terminal hydrolase 48Add BLAST | 1052 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 903 | PhosphoserineBy similarity | 1 | |
Modified residuei | 904 | PhosphoserineBy similarity | 1 | |
Modified residuei | 905 | PhosphoserineBy similarity | 1 | |
Modified residuei | 973 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q3V0C5 |
jPOSTi | Q3V0C5 |
MaxQBi | Q3V0C5 |
PaxDbi | Q3V0C5 |
PeptideAtlasi | Q3V0C5 |
PRIDEi | Q3V0C5 |
ProteomicsDBi | 298107 [Q3V0C5-1] 298108 [Q3V0C5-2] 298109 [Q3V0C5-3] 298110 [Q3V0C5-4] 298111 [Q3V0C5-5] |
PTM databases
iPTMneti | Q3V0C5 |
PhosphoSitePlusi | Q3V0C5 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000043411, Expressed in retinal neural layer and 314 other tissues |
ExpressionAtlasi | Q3V0C5, baseline and differential |
Genevisiblei | Q3V0C5, MM |
Interactioni
Subunit structurei
Interacts with TRAF2 and RELA.
By similarityProtein-protein interaction databases
BioGRIDi | 228386, 4 interactors |
STRINGi | 10090.ENSMUSP00000055016 |
Miscellaneous databases
RNActi | Q3V0C5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 89 – 420 | USPAdd BLAST | 332 | |
Domaini | 459 – 553 | DUSP 1PROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 568 – 691 | DUSP 2PROSITE-ProRule annotationAdd BLAST | 124 | |
Domaini | 710 – 841 | DUSP 3PROSITE-ProRule annotationAdd BLAST | 132 | |
Domaini | 946 – 1026 | Ubiquitin-likePROSITE-ProRule annotationAdd BLAST | 81 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 609 – 639 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 896 – 940 | DisorderedSequence analysisAdd BLAST | 45 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 904 – 922 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 923 – 940 | Polar residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1863, Eukaryota |
GeneTreei | ENSGT00940000156015 |
InParanoidi | Q3V0C5 |
OrthoDBi | 113272at2759 |
PhylomeDBi | Q3V0C5 |
TreeFami | TF106280 |
Family and domain databases
CDDi | cd02668, Peptidase_C19L, 1 hit cd01795, Ubl_USP48, 1 hit |
InterProi | View protein in InterPro IPR035927, DUSP-like_sf IPR038765, Papain-like_cys_pep_sf IPR006615, Pept_C19_DUSP IPR001394, Peptidase_C19_UCH IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR044743, Ubl_USP48 IPR033841, USP48 IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF00443, UCH, 1 hit |
SUPFAMi | SSF143791, SSF143791, 1 hit SSF54001, SSF54001, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS51283, DUSP, 3 hits PS50053, UBIQUITIN_2, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 12 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPRLQLEKA AWRWAETVRP EEVSQEHIET AYRIWLEPCI RGVCRRNCRG
60 70 80 90 100
NPNCLVGIGE HIWLGEIDEN SFHSIDDPNC ERRKKNSFVG LTNLGATCYV
110 120 130 140 150
NTFLQVWFLN LELRQALYLC PSTCSDYTKG DGIHGGKDYE PQTICEHLQY
160 170 180 190 200
LFALLQNSNR RYIDPSGFVK ALGLDTGQQQ DAQEFSKLFM SLLEDTLSKQ
210 220 230 240 250
KNPDVRNVVQ QQFCGEYAYV TVCNQCGRES KLVSKFYELE LNIQGHKQLT
260 270 280 290 300
DCISEFLKEE RLEGDNRYFC ENCQSKQNAT RKIRLLSLPC TLNLQLMRFV
310 320 330 340 350
FDRQTGHKKK LNAYIGFSES LDMEPYVEHK GGSFVYELSA VLIHRGVSAY
360 370 380 390 400
SGHYIAHVKD PQSGEWYKFN DEDIEKMEGK KLQLGIEEDL EPSKSQTRKP
410 420 430 440 450
KCGKGTHCSR NAYMLVYRLQ AQEKNHTMVQ VPAFLQELVD RDNSKFEEWC
460 470 480 490 500
VEMAEMRKQS VDKGKAKHEE VKELYQRLPA GAEPYEFVSL EWLQKWLDES
510 520 530 540 550
TPTKPIDNNA CLCSHDKLHP DKISIMKRIS EYAANIFYSR YGGGPRLTVK
560 570 580 590 600
ALCKDCVVER CRVLRLKNQL NEDYKAVNNL LKSTMKGDGF WVGKSSLRSW
610 620 630 640 650
RQLALEQLDE QDGEAEQSNG KINGSTFNKD ESKEEKKEEE EELNFNEDIL
660 670 680 690 700
CPHGELSISE NERRLVSQEA WSKLQQYFPK APEFPSYREC CSQCKILERE
710 720 730 740 750
GEENEALHKM IANEQKTSLP NLFQDKNRPC LSNWPEDTDA LYIVSHFFLD
760 770 780 790 800
EWRKFVRKPA RSAPVSSIGN AALLCPHGGL MFTFPSLTKE DSKLCQPLER
810 820 830 840 850
REGGWPTAAH NNIALIWPSE WQMIQKLFVV DKVIKITRIE VGDVNPSQTQ
860 870 880 890 900
YISEPSLCPD CREGLLCQQQ RDLREYTQAT IYVHKVVDNK KVMKDSAPEL
910 920 930 940 950
NVSSSETEED KEEAKPDGEK DPDFNQSNGS TKRQKTSQQG YVAYQKQVIR
960 970 980 990 1000
RSTRHRKVRG EKALLVSANQ TLKELKIQIM HAFSVAPFDQ NLSIDGKILN
1010 1020 1030 1040 1050
DDCATLGTLG VIPESVILLK ADEPIADYAA MDDVMQVCMP EEGFKGTGLL
GH
Computationally mapped potential isoform sequencesi
There are 12 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2ALR9 | A2ALR9_MOUSE | Ubiquitinyl hydrolase 1 | Usp48 | 1,036 | Annotation score: | ||
A2ALR8 | A2ALR8_MOUSE | Ubiquitinyl hydrolase 1 | Usp48 | 631 | Annotation score: | ||
A2ALR5 | A2ALR5_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp48 | 267 | Annotation score: | ||
F6VSP0 | F6VSP0_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp48 | 156 | Annotation score: | ||
F7ALR2 | F7ALR2_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp48 | 113 | Annotation score: | ||
F6TH61 | F6TH61_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp48 | 123 | Annotation score: | ||
A2BDP2 | A2BDP2_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp48 | 165 | Annotation score: | ||
F6Z2T3 | F6Z2T3_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp48 | 87 | Annotation score: | ||
A2ALS9 | A2ALS9_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp48 | 106 | Annotation score: | ||
F6TGT8 | F6TGT8_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp48 | 170 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020482 | 1 | M → MVGPCPFGRRRRRRRRPPGS ASATSVKAM in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_020483 | 390 | L → LA in isoform 3 and isoform 5. 2 Publications | 1 | |
Alternative sequenceiVSP_020484 | 483 – 484 | EP → GL in isoform 3 and isoform 4. 2 Publications | 2 | |
Alternative sequenceiVSP_020485 | 485 – 1052 | Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST | 568 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK220190 mRNA Translation: BAD90375.1 Different initiation. AK133251 mRNA Translation: BAE21579.1 AK166158 mRNA Translation: BAE38603.1 BC021769 mRNA Translation: AAH21769.1 Frameshift. |
CCDSi | CCDS38924.1 [Q3V0C5-1] |
RefSeqi | NP_570949.2, NM_130879.2 [Q3V0C5-1] XP_017175495.1, XM_017320006.1 [Q3V0C5-5] |
Genome annotation databases
Ensembli | ENSMUST00000055131; ENSMUSP00000055016; ENSMUSG00000043411 [Q3V0C5-1] |
GeneIDi | 170707 |
KEGGi | mmu:170707 |
UCSCi | uc008vjh.1, mouse [Q3V0C5-2] uc008vji.1, mouse [Q3V0C5-3] uc008vjj.1, mouse [Q3V0C5-4] uc008vjl.1, mouse [Q3V0C5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK220190 mRNA Translation: BAD90375.1 Different initiation. AK133251 mRNA Translation: BAE21579.1 AK166158 mRNA Translation: BAE38603.1 BC021769 mRNA Translation: AAH21769.1 Frameshift. |
CCDSi | CCDS38924.1 [Q3V0C5-1] |
RefSeqi | NP_570949.2, NM_130879.2 [Q3V0C5-1] XP_017175495.1, XM_017320006.1 [Q3V0C5-5] |
3D structure databases
SMRi | Q3V0C5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228386, 4 interactors |
STRINGi | 10090.ENSMUSP00000055016 |
Protein family/group databases
MEROPSi | C19.068 |
PTM databases
iPTMneti | Q3V0C5 |
PhosphoSitePlusi | Q3V0C5 |
Proteomic databases
EPDi | Q3V0C5 |
jPOSTi | Q3V0C5 |
MaxQBi | Q3V0C5 |
PaxDbi | Q3V0C5 |
PeptideAtlasi | Q3V0C5 |
PRIDEi | Q3V0C5 |
ProteomicsDBi | 298107 [Q3V0C5-1] 298108 [Q3V0C5-2] 298109 [Q3V0C5-3] 298110 [Q3V0C5-4] 298111 [Q3V0C5-5] |
Protocols and materials databases
Antibodypediai | 29995, 194 antibodies from 28 providers |
DNASUi | 170707 |
Genome annotation databases
Ensembli | ENSMUST00000055131; ENSMUSP00000055016; ENSMUSG00000043411 [Q3V0C5-1] |
GeneIDi | 170707 |
KEGGi | mmu:170707 |
UCSCi | uc008vjh.1, mouse [Q3V0C5-2] uc008vji.1, mouse [Q3V0C5-3] uc008vjj.1, mouse [Q3V0C5-4] uc008vjl.1, mouse [Q3V0C5-1] |
Organism-specific databases
CTDi | 84196 |
MGIi | MGI:2158502, Usp48 |
VEuPathDBi | HostDB:ENSMUSG00000043411 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG1863, Eukaryota |
GeneTreei | ENSGT00940000156015 |
InParanoidi | Q3V0C5 |
OrthoDBi | 113272at2759 |
PhylomeDBi | Q3V0C5 |
TreeFami | TF106280 |
Enzyme and pathway databases
Reactomei | R-MMU-5689880, Ub-specific processing proteases |
Miscellaneous databases
BioGRID-ORCSi | 170707, 0 hits in 49 CRISPR screens |
ChiTaRSi | Usp48, mouse |
PROi | PR:Q3V0C5 |
RNActi | Q3V0C5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000043411, Expressed in retinal neural layer and 314 other tissues |
ExpressionAtlasi | Q3V0C5, baseline and differential |
Genevisiblei | Q3V0C5, MM |
Family and domain databases
CDDi | cd02668, Peptidase_C19L, 1 hit cd01795, Ubl_USP48, 1 hit |
InterProi | View protein in InterPro IPR035927, DUSP-like_sf IPR038765, Papain-like_cys_pep_sf IPR006615, Pept_C19_DUSP IPR001394, Peptidase_C19_UCH IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR044743, Ubl_USP48 IPR033841, USP48 IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF00443, UCH, 1 hit |
SUPFAMi | SSF143791, SSF143791, 1 hit SSF54001, SSF54001, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS51283, DUSP, 3 hits PS50053, UBIQUITIN_2, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UBP48_MOUSE | |
Accessioni | Q3V0C5Primary (citable) accession number: Q3V0C5 Secondary accession number(s): Q3TM39, Q571J9, Q8VDJ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2006 |
Last sequence update: | September 19, 2006 | |
Last modified: | February 23, 2022 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families