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Protein

GPI inositol-deacylase

Gene

Pgap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei174By similarity1

GO - Molecular functioni

  • nuclease activity Source: UniProtKB
  • phosphatidylinositol deacylase activity Source: GO_Central
  • phosphoric ester hydrolase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-162791 Attachment of GPI anchor to uPAR

Protein family/group databases

ESTHERimouse-q3uuq7 PGAP1

Names & Taxonomyi

Protein namesi
Recommended name:
GPI inositol-deacylase (EC:3.1.-.-)
Alternative name(s):
Post-GPI attachment to proteins factor 1
Gene namesi
Name:Pgap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2443342 Pgap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Topological domaini33 – 597LumenalSequence analysisAdd BLAST565
Transmembranei598 – 618HelicalSequence analysisAdd BLAST21
Topological domaini619 – 641CytoplasmicSequence analysisAdd BLAST23
Transmembranei642 – 662HelicalSequence analysisAdd BLAST21
Topological domaini663 – 668LumenalSequence analysis6
Transmembranei669 – 689HelicalSequence analysisAdd BLAST21
Topological domaini690 – 694CytoplasmicSequence analysis5
Transmembranei695 – 715HelicalSequence analysisAdd BLAST21
Topological domaini716 – 733LumenalSequence analysisAdd BLAST18
Transmembranei734 – 754HelicalSequence analysisAdd BLAST21
Topological domaini755 – 817CytoplasmicSequence analysisAdd BLAST63
Transmembranei818 – 838HelicalSequence analysisAdd BLAST21
Topological domaini839 – 894LumenalSequence analysisAdd BLAST56
Transmembranei895 – 915HelicalSequence analysisAdd BLAST21
Topological domaini916 – 922CytoplasmicSequence analysis7

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3259507

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002776241 – 922GPI inositol-deacylaseAdd BLAST922

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi363N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi402N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi558N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3UUQ7
PeptideAtlasiQ3UUQ7
PRIDEiQ3UUQ7

PTM databases

iPTMnetiQ3UUQ7
PhosphoSitePlusiQ3UUQ7

Expressioni

Gene expression databases

BgeeiENSMUSG00000073678 Expressed in 230 organ(s), highest expression level in forebrain
CleanExiMM_PGAP1
GenevisibleiQ3UUQ7 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095346

Structurei

3D structure databases

ProteinModelPortaliQ3UUQ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI inositol-deacylase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3724 Eukaryota
ENOG410YD9N LUCA
GeneTreeiENSGT00390000016484
HOGENOMiHOG000090006
HOVERGENiHBG082122
InParanoidiQ3UUQ7
KOiK05294
OMAiCRYRISY
OrthoDBiEOG091G06G8
TreeFamiTF314565

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR012908 PGAP1-like
IPR039529 PGAP1/BST1
PANTHERiPTHR15495 PTHR15495, 1 hit
PfamiView protein in Pfam
PF07819 PGAP1, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00120 LIPASE_SER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q3UUQ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MFLHSVNLWN LAFYVFMVFL ATLGLWDVFF GFEENKCSMS YMFEYPEYQK
60 70 80 90 100
IELPKKLTKR YPAYELYLYG EGSYAEEHKI LPLTGIPVLF LPGNAGSYKQ
110 120 130 140 150
VRSIGSIALR KAEDIDFKYH FDFFSVNFNE ELVALYGGSL QKQTKFVHEC
160 170 180 190 200
IKAILKLYKG QEFAPTSVAI IGHSMGGLVA RALLTLKNFK QDLINLLVTQ
210 220 230 240 250
ATPHVAPVMP LDRFITEFYM NVNNYWILNA RHINLTTLSV AGGFRDYQVR
260 270 280 290 300
SGLTFLPKLS HYTSALSVVS SAVPKTWVST DHLSIVWCKQ LQLTTIRAFF
310 320 330 340 350
DLIDADTKQI TQKPKKKLSV LNHHFIRHPA KQFEENPSII SDLTGTSMWV
360 370 380 390 400
PVKVSRWSYV AYNESDKIYF AFPLANHRKI YTHAYCQSTM LDTNSWIFGC
410 420 430 440 450
INSTSMCRQG VDLSWKAELL PTIKSLTLRL QDYPSLSHIV VYVPSVHGSK
460 470 480 490 500
FVVDCEFFKK EARSMQLPVT HLFSFGLSSR KVTLNTNGLY YNIELLNFGQ
510 520 530 540 550
IYQAFKVNVV SKCTGSKEEI TSIYKLHIPW SYEDSLTIAQ VPSSTDISLK
560 570 580 590 600
LHVAQPENDS HVALLKMYTS SDCQYEVTIK TSFPQILGQV VRFHGGALPA
610 620 630 640 650
YVVSSILLAY GGQLYSLLST GYCLEYSTIL DKEAKPYKVD PFVIMIKFLL
660 670 680 690 700
GYKWFKELWD AVLLPELDAI VLTSQSMCFP LVSLILFLFG TCTAYWSGLL
710 720 730 740 750
SSTSVQLLSS LWLALKRPAE LPKDIKVMSP DLPVLTVVFL IVSWTTCGAL
760 770 780 790 800
AILLSYLYYV FKVVHLQASL TTFKNNQPVN PKHSRRSEKK SNHHKDSAVQ
810 820 830 840 850
SLRLCANDAE DSLRMHSTVI NLLTWVVLLS MPSLIYWLKN LRYYFKLSPD
860 870 880 890 900
PCKPLAFLLI PAIAILGNTH TVSVKSSKLL KTVSQFPLPL AVGVIAFGSS
910 920
HLYRVPCFVI IPLVFHALCN FM
Length:922
Mass (Da):104,578
Last modified:July 27, 2011 - v3
Checksum:iE503CA512882F943
GO
Isoform 2 (identifier: Q3UUQ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-166: KIELPKKLTK...LYKGQEFAPT → VRPSLTCAPT...VAKADLKKRL
     167-922: Missing.

Note: No experimental confirmation available.
Show »
Length:166
Mass (Da):18,488
Checksum:i23862438C15C3B39
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223N → D in BAE23568 (PubMed:16141072).Curated1
Sequence conflicti576E → V in BAE23568 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02304550 – 166KIELP…EFAPT → VRPSLTCAPTPPGLLSGSDG TLRLGALLALEVSCFVLGRA SSPPRPLVPHPDSGPRLLTA REPALVPLPNPYTSRTLLCL QGDDFSSRPVMGVRQISLFW KEAASCRVAKADLKKRL in isoform 2. 1 PublicationAdd BLAST117
Alternative sequenceiVSP_023046167 – 922Missing in isoform 2. 1 PublicationAdd BLAST756

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051360 mRNA Translation: BAC34614.1
AK138171 mRNA Translation: BAE23568.1
AC121980 Genomic DNA No translation available.
AC124538 Genomic DNA No translation available.
CCDSiCCDS48260.1 [Q3UUQ7-1]
RefSeqiNP_001156786.1, NM_001163314.2 [Q3UUQ7-1]
UniGeneiMm.103539

Genome annotation databases

EnsembliENSMUST00000097739; ENSMUSP00000095346; ENSMUSG00000073678 [Q3UUQ7-1]
GeneIDi241062
KEGGimmu:241062
UCSCiuc011wky.2 mouse [Q3UUQ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051360 mRNA Translation: BAC34614.1
AK138171 mRNA Translation: BAE23568.1
AC121980 Genomic DNA No translation available.
AC124538 Genomic DNA No translation available.
CCDSiCCDS48260.1 [Q3UUQ7-1]
RefSeqiNP_001156786.1, NM_001163314.2 [Q3UUQ7-1]
UniGeneiMm.103539

3D structure databases

ProteinModelPortaliQ3UUQ7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095346

Chemistry databases

ChEMBLiCHEMBL3259507

Protein family/group databases

ESTHERimouse-q3uuq7 PGAP1

PTM databases

iPTMnetiQ3UUQ7
PhosphoSitePlusiQ3UUQ7

Proteomic databases

PaxDbiQ3UUQ7
PeptideAtlasiQ3UUQ7
PRIDEiQ3UUQ7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097739; ENSMUSP00000095346; ENSMUSG00000073678 [Q3UUQ7-1]
GeneIDi241062
KEGGimmu:241062
UCSCiuc011wky.2 mouse [Q3UUQ7-1]

Organism-specific databases

CTDi80055
MGIiMGI:2443342 Pgap1

Phylogenomic databases

eggNOGiKOG3724 Eukaryota
ENOG410YD9N LUCA
GeneTreeiENSGT00390000016484
HOGENOMiHOG000090006
HOVERGENiHBG082122
InParanoidiQ3UUQ7
KOiK05294
OMAiCRYRISY
OrthoDBiEOG091G06G8
TreeFamiTF314565

Enzyme and pathway databases

ReactomeiR-MMU-162791 Attachment of GPI anchor to uPAR

Miscellaneous databases

PROiPR:Q3UUQ7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000073678 Expressed in 230 organ(s), highest expression level in forebrain
CleanExiMM_PGAP1
GenevisibleiQ3UUQ7 MM

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR012908 PGAP1-like
IPR039529 PGAP1/BST1
PANTHERiPTHR15495 PTHR15495, 1 hit
PfamiView protein in Pfam
PF07819 PGAP1, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00120 LIPASE_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPGAP1_MOUSE
AccessioniPrimary (citable) accession number: Q3UUQ7
Secondary accession number(s): E9QKG7, Q8BQ77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 95 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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