UniProtKB - Q3UTY6 (THSD4_MOUSE)
Protein
Thrombospondin type-1 domain-containing protein 4
Gene
Thsd4
Organism
Mus musculus (Mouse)
Status
Functioni
Promotes FBN1 matrix assembly. Attenuates TGFB signaling, possibly by accelerating the sequestration of large latent complexes of TGFB or active TGFB by FBN1 microfibril assembly, thereby negatively regulating the expression of TGFB regulatory targets, such as POSTN.3 Publications
GO - Molecular functioni
- metalloendopeptidase activity Source: GO_Central
GO - Biological processi
- elastic fiber assembly Source: MGI
- extracellular matrix organization Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
Reactomei | R-MMU-5173214, O-glycosylation of TSR domain-containing proteins |
Names & Taxonomyi
Protein namesi | Recommended name: Thrombospondin type-1 domain-containing protein 4Alternative name(s): A disintegrin and metalloproteinase with thrombospondin motifs-like protein 6 Short name: ADAMTS-like protein 6 Short name: ADAMTSL-6 ADAMTS-like protein 4 Short name: ADAMTSL-4 |
Gene namesi | Name:Thsd4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2672033, Thsd4 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix 2 Publications
Note: Mostly deposited into pericellular fibrillar matrices (PubMed:19940141). Colocalizes with FBN1 (PubMed:19940141).1 Publication
Extracellular region or secreted
- Secreted 1 Publication
- extracellular matrix 2 Publications
Note: Mostly secreted in the extracellular milieu (PubMed:19940141). In the pericellular fibrillar matrix, colocalizes with FBN1 (PubMed:19940141) (PubMed:21880733).2 Publications
Extracellular region or secreted
- extracellular matrix Source: MGI
- microfibril Source: MGI
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000313584 | 27 – 1018 | Thrombospondin type-1 domain-containing protein 4Add BLAST | 992 |
Proteomic databases
PaxDbi | Q3UTY6 |
PRIDEi | Q3UTY6 |
PTM databases
GlyConnecti | 2766, 2 N-Linked glycans (1 site) |
GlyGeni | Q3UTY6, 1 site |
iPTMneti | Q3UTY6 |
PhosphoSitePlusi | Q3UTY6 |
Expressioni
Tissue specificityi
Both isoforms are expressed in the embryo from 7 dpc through 17. Isoform 1 is widely expressed in adult tissues. Isoform 2 is detected in brain, spinal cord, eye, kidney, stomach and uterus. Mainly observed in fibrillar extracellular matrices in elastic tissues (at protein level).1 Publication
Developmental stagei
At 16.5 dpc, detected in fibrillar structures in various elastic tissues, including developing dermis, perichondria surrounding cartilages and the vessel walls of aortae (at protein level). At postnatal day 7 (P7), weakly expressed in the early stage dental follicle, but becomes readily detectable in assembled microfibril-like structures during the periodontal ligament-forming stage of the dental follicle and in organized microfibrils in the adult periodontal ligament (P35) (at protein level). Up-regulated in the periodontal ligament during wound healing (at protein level).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000032289, Expressed in ascending aorta and 179 other tissues |
ExpressionAtlasi | Q3UTY6, baseline and differential |
Genevisiblei | Q3UTY6, MM |
Interactioni
Subunit structurei
Isoform 2 interacts with FBN1. Isoform 2 may interact with TGFB1.
2 PublicationsProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000096257 |
Miscellaneous databases
RNActi | Q3UTY6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 54 – 307 | TSP type-1 1PROSITE-ProRule annotationAdd BLAST | 254 | |
Domaini | 676 – 737 | TSP type-1 2PROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 739 – 792 | TSP type-1 3PROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 793 – 851 | TSP type-1 4PROSITE-ProRule annotationAdd BLAST | 59 | |
Domaini | 852 – 911 | TSP type-1 5PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 912 – 968 | TSP type-1 6PROSITE-ProRule annotationAdd BLAST | 57 | |
Domaini | 971 – 1008 | PLACPROSITE-ProRule annotationAdd BLAST | 38 |
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG3538, Eukaryota KOG4597, Eukaryota |
GeneTreei | ENSGT00940000156594 |
HOGENOMi | CLU_000660_6_0_1 |
InParanoidi | Q3UTY6 |
OMAi | NRCSAEC |
OrthoDBi | 414258at2759 |
PhylomeDBi | Q3UTY6 |
TreeFami | TF316874 |
Family and domain databases
Gene3Di | 2.20.100.10, 7 hits |
InterProi | View protein in InterPro IPR010294, ADAM_spacer1 IPR010909, PLAC IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF05986, ADAM_spacer1, 1 hit PF08686, PLAC, 1 hit PF00090, TSP_1, 1 hit |
SMARTi | View protein in SMART SM00209, TSP1, 7 hits |
SUPFAMi | SSF82895, SSF82895, 7 hits |
PROSITEi | View protein in PROSITE PS50900, PLAC, 1 hit PS50092, TSP1, 6 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative promoter usage. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q3UTY6-1) [UniParc]FASTAAdd to basket
Also known as: ADAMTSL6-alpha
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVSYLTSCLS ALSTLLLLLG SQLVCPQPST EHRKVPQRMA VTEGTPEDSG
60 70 80 90 100
SGSPGVWGSW GPWSACSRSC SGGVMEQTRP CLPSSYRARG GSRPNGRALS
110 120 130 140 150
ITGHVVSAVR TSVPLHRSQE DQRALAGSNA SRQGPAVVRG SRHPQARGRE
160 170 180 190 200
PSERRSRTRG PIGPGKYGYG KAPYILPLQT DTTHTPQRLR RQRPSSRHSR
210 220 230 240 250
SQEASASKQG YRPPTHQFSH SQPLYQSDSG PRSGLPPSEA SIYQLPLTHD
260 270 280 290 300
QSYPAASSLF HRPELSSHHG ARPHGAAQAF PQHLRSTAIS CIGAYRQYKL
310 320 330 340 350
CNTNACPESG RSIREVQCAS YNNKPFMGRF YEWEPFAEVK GNRKCELNCQ
360 370 380 390 400
ATGYRFYVRQ AEKVIDGTPC DQNGTAICVS GQCKSIGCDD FLGSDKVLDK
410 420 430 440 450
CGVCGGDNTG CQVVSGVFKH ALTSLGYHRV VEIPQGATKI NITEMHKSNN
460 470 480 490 500
YLALRSRSGR SIINGNWAID RPGKYEGGGT MFTYKRPNEV SSTAGESFLA
510 520 530 540 550
EGPTNEILDV YMIHQQPNPG VHYEYVIMRN NAISPQVPPH RRPGEPFNGQ
560 570 580 590 600
LEEEDRGQED REEREKNQEK EDSQVEAPEV FTSESTQTFP VRHPERFPSH
610 620 630 640 650
RPDNLVPPAP QPPRRSRDHN WKQLGTTECS TTCGKGSQYP IFRCVHRNTH
660 670 680 690 700
EEVPESYCDS SMKPTPEEEP CNLFPCPAFW DIGEWSECSK TCGLGMQHRQ
710 720 730 740 750
VLCRQVYANR SLTVQPYRCQ HLEKPETTST CQLKICSEWQ IRTDWTSCSV
760 770 780 790 800
PCGVGQRTRD VKCVSNIGDM VHDEECNMKL RPNDIENCDM GPCAKSWFLT
810 820 830 840 850
EWSERCSAEC GAGVRTRSVV CMTNHVSSLP LEGCGNNRPV EATPCDNGPC
860 870 880 890 900
TGKVEWFTGS WSQCSIECGS GTQQREVICV RKNADTFEVL DPYECSFLEK
910 920 930 940 950
PPSQQACHLK PCGAKWFSTE WSMCSKSCQG GFRVREVRCL SDDMTPSSLC
960 970 980 990 1000
DPQLKPEERE SCNTQDCVPE VDENCKDKYY NCNVVVQARL CVYNYYKTAC
1010
CASCTRVANR HVGFLGSR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3Z044 | D3Z044_MOUSE | Thrombospondin type-1 domain-contai... | Thsd4 | 134 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 486 | R → Q in BAE23844 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 571 | E → K in BAE23844 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_030042 | 1 – 360 | Missing in isoform 2. 1 PublicationAdd BLAST | 360 | |
Alternative sequenceiVSP_030043 | 361 – 384 | AEKVI…SGQCK → MFVSYLLLTLFHTETAMLAR PGGE in isoform 2. 1 PublicationAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK045414 mRNA Translation: BAC32352.1 AK138976 mRNA Translation: BAE23844.1 |
CCDSi | CCDS23256.1 [Q3UTY6-2] CCDS40661.1 [Q3UTY6-1] |
RefSeqi | NP_001035516.2, NM_001040426.3 [Q3UTY6-1] NP_766032.1, NM_172444.4 [Q3UTY6-2] XP_006510969.1, XM_006510906.3 [Q3UTY6-1] XP_006510970.1, XM_006510907.3 [Q3UTY6-1] |
Genome annotation databases
Ensembli | ENSMUST00000034829; ENSMUSP00000034829; ENSMUSG00000032289 [Q3UTY6-2] ENSMUST00000098660; ENSMUSP00000096257; ENSMUSG00000032289 [Q3UTY6-1] ENSMUST00000171654; ENSMUSP00000131418; ENSMUSG00000032289 [Q3UTY6-1] |
GeneIDi | 207596 |
KEGGi | mmu:207596 |
UCSCi | uc009pyx.2, mouse [Q3UTY6-2] uc009pyy.2, mouse [Q3UTY6-1] |
Keywords - Coding sequence diversityi
Alternative promoter usageSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK045414 mRNA Translation: BAC32352.1 AK138976 mRNA Translation: BAE23844.1 |
CCDSi | CCDS23256.1 [Q3UTY6-2] CCDS40661.1 [Q3UTY6-1] |
RefSeqi | NP_001035516.2, NM_001040426.3 [Q3UTY6-1] NP_766032.1, NM_172444.4 [Q3UTY6-2] XP_006510969.1, XM_006510906.3 [Q3UTY6-1] XP_006510970.1, XM_006510907.3 [Q3UTY6-1] |
3D structure databases
SMRi | Q3UTY6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000096257 |
PTM databases
GlyConnecti | 2766, 2 N-Linked glycans (1 site) |
GlyGeni | Q3UTY6, 1 site |
iPTMneti | Q3UTY6 |
PhosphoSitePlusi | Q3UTY6 |
Proteomic databases
PaxDbi | Q3UTY6 |
PRIDEi | Q3UTY6 |
Protocols and materials databases
Antibodypediai | 26512, 7 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034829; ENSMUSP00000034829; ENSMUSG00000032289 [Q3UTY6-2] ENSMUST00000098660; ENSMUSP00000096257; ENSMUSG00000032289 [Q3UTY6-1] ENSMUST00000171654; ENSMUSP00000131418; ENSMUSG00000032289 [Q3UTY6-1] |
GeneIDi | 207596 |
KEGGi | mmu:207596 |
UCSCi | uc009pyx.2, mouse [Q3UTY6-2] uc009pyy.2, mouse [Q3UTY6-1] |
Organism-specific databases
CTDi | 79875 |
MGIi | MGI:2672033, Thsd4 |
Phylogenomic databases
eggNOGi | KOG3538, Eukaryota KOG4597, Eukaryota |
GeneTreei | ENSGT00940000156594 |
HOGENOMi | CLU_000660_6_0_1 |
InParanoidi | Q3UTY6 |
OMAi | NRCSAEC |
OrthoDBi | 414258at2759 |
PhylomeDBi | Q3UTY6 |
TreeFami | TF316874 |
Enzyme and pathway databases
Reactomei | R-MMU-5173214, O-glycosylation of TSR domain-containing proteins |
Miscellaneous databases
BioGRID-ORCSi | 207596, 1 hit in 17 CRISPR screens |
ChiTaRSi | Thsd4, mouse |
PROi | PR:Q3UTY6 |
RNActi | Q3UTY6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032289, Expressed in ascending aorta and 179 other tissues |
ExpressionAtlasi | Q3UTY6, baseline and differential |
Genevisiblei | Q3UTY6, MM |
Family and domain databases
Gene3Di | 2.20.100.10, 7 hits |
InterProi | View protein in InterPro IPR010294, ADAM_spacer1 IPR010909, PLAC IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF05986, ADAM_spacer1, 1 hit PF08686, PLAC, 1 hit PF00090, TSP_1, 1 hit |
SMARTi | View protein in SMART SM00209, TSP1, 7 hits |
SUPFAMi | SSF82895, SSF82895, 7 hits |
PROSITEi | View protein in PROSITE PS50900, PLAC, 1 hit PS50092, TSP1, 6 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THSD4_MOUSE | |
Accessioni | Q3UTY6Primary (citable) accession number: Q3UTY6 Secondary accession number(s): Q8BLE5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | January 15, 2008 | |
Last modified: | December 2, 2020 | |
This is version 114 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot